Photosynthetic Conversion of Carbon Dioxide to Oleochemicals by Cyanobacteria: Recent Advances and Future Perspectives

Wang, Li; Li-yuan, Chen; Yang, Shihui; Tan, Xiaoming

doi:10.3389/fmicb.2020.00634

Cited by 23 publications

(15 citation statements)

References 118 publications

(190 reference statements)

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“…The supplementation of 16:0 and 18:0 had positive effects on the photoinhibition of PSII ( Figure 1 ). Synechocystis does not have the complete metabolic cycle for the degradation of fatty acids, such as β-oxidation [ 27 ], indicating that exogeneous FFA might not be metabolized or used for the substrates for the respiration in the cells. In the cycle of fatty acid synthesis, 16:0 is the substrate for elongation to 18:0.…”

Section: Discussionmentioning

confidence: 99%

Long-Chain Saturated Fatty Acids, Palmitic and Stearic Acids, Enhance the Repair of Photosystem II

Jimbo

Takagi

Hirashima

et al. 2020

IJMS

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Free fatty acids (FFA) generated in cyanobacterial cells can be utilized for the biodiesel that is required for our sustainable future. The combination of FFA and strong light induces severe photoinhibition of photosystem II (PSII), which suppresses the production of FFA in cyanobacterial cells. In the present study, we examined the effects of exogenously added FFA on the photoinhibition of PSII in Synechocystis sp. PCC 6803. The addition of lauric acid (12:0) to cells accelerated the photoinhibition of PSII by inhibiting the repair of PSII and the de novo synthesis of D1. α-Linolenic acid (18:3) affected both the repair of and photodamage to PSII. Surprisingly, palmitic (16:0) and stearic acids (18:0) enhanced the repair of PSII by accelerating the de novo synthesis of D1 with the mitigation of the photoinhibition of PSII. Our results show chemical potential of FFA in the regulation of PSII without genetic manipulation.

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Section: Discussionmentioning

confidence: 99%

Long-Chain Saturated Fatty Acids, Palmitic and Stearic Acids, Enhance the Repair of Photosystem II

Jimbo

Takagi

Hirashima

et al. 2020

IJMS

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“…FFA production using cyanobacteria is considered to be one of the promising approaches to sustainable production of biofuels ( Wang et al. 2020 ).…”

Section: Discussionmentioning

confidence: 99%

High-Light-Induced Stress Activates Lipid Deacylation at the Sn-2 Position in the Cyanobacterium Synechocystis Sp. PCC 6803

Kojima

Matsumoto

Keta

et al. 2021

Plant and Cell Physiology

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Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) produce free fatty acids (FFAs) because the FFAs generated by deacylation of membrane lipids cannot be recycled. An engineered Aas-deficient mutant of Synechocystis sp. PCC 6803 grew normally under low-light (LL) conditions (50 µmol photons m-2 s-1) but was unable to sustain growth under high-light (HL) conditions (400 µmol photons m-2 s-1), revealing a crucial role of Aas in survival under the HL conditions. Several-times larger amounts of FFAs were produced by HL-exposed cultures than LL-grown cultures. Palmitic acid accounted for ~85% of total FFAs in HL-exposed cultures, while C18 fatty acids constituted ~80% of the FFAs in LL-grown cultures. Since C16 fatty acids are esterified to the sn-2 position of lipids in the Synechocystis species, it was deduced that HL irradiation activated deacylation of lipids at the sn-2 position. Heterologous expression of FarB, the FFA exporter protein of Neisseria lactamica, prevented intracellular FFA accumulation and rescued the growth defect of the mutant under HL, indicating that intracellular FFA was the cause of growth inhibition. FarB expression also decreased the per-cell yield of FFA under HL by 90% and decreased the proportion of palmitic acid to ~15% of total FFA. These results indicated that the HL-induced lipid deacylation is triggered not by strong light per se but by HL-induced damage to the cells. It was deduced that there is a positive feedback loop between HL-induced damage and lipid deacylation, which is lethal unless FFA accumulation is prevented by Aas.

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“…Their photoautotrophic metabolism enables the sequestration and conversion of atmospheric CO 2 into organic compounds using sunlight and water as energy and electron sources, respectively (Knoll, 2008;Ananya and Ahmad, 2014). Therefore, they are being increasingly studied to be used as solar-powered cell factories for many biotechnological applications including the production of e.g., alcohols, alkanes, hydrogen, sugars, and terpenoids (Hays and Ducat, 2015;Lindblad, 2018;Sadvakasova et al, 2020;Wang et al, 2020;Rodrigues and Lindberg, 2021). Among cyanobacteria, the unicellular Synechocystis sp.…”

Section: Introductionmentioning

confidence: 99%

Heterologous Production of Glycine Betaine Using Synechocystis sp. PCC 6803-Based Chassis Lacking Native Compatible Solutes

Ferreira

Pacheco

Rodrigues

et al. 2022

Front. Bioeng. Biotechnol.

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Among compatible solutes, glycine betaine has various applications in the fields of nutrition, pharmaceuticals, and cosmetics. Currently, this compound can be extracted from sugar beet plants or obtained by chemical synthesis, resulting in low yields or high carbon footprint, respectively. Hence, in this work we aimed at exploring the production of glycine betaine using the unicellular cyanobacterium Synechocystis sp. PCC 6803 as a photoautotrophic chassis. Synechocystis mutants lacking the native compatible solutes sucrose or/and glucosylglycerol—∆sps, ∆ggpS, and ∆sps∆ggpS—were generated and characterized. Under salt stress conditions, the growth was impaired and accumulation of glycogen decreased by ∼50% whereas the production of compatible solutes and extracellular polymeric substances (capsular and released ones) increased with salinity. These mutants were used as chassis for the implementation of a synthetic device based on the metabolic pathway described for the halophilic cyanobacterium Aphanothece halophytica for the production of the compatible solute glycine betaine. Transcription of ORFs comprising the device was shown to be stable and insulated from Synechocystis’ native regulatory network. Production of glycine betaine was achieved in all chassis tested, and was shown to increase with salinity. The introduction of the glycine betaine synthetic device into the ∆ggpS background improved its growth and enabled survival under 5% NaCl, which was not observed in the absence of the device. The maximum glycine betaine production [64.29 µmol/gDW (1.89 µmol/mg protein)] was reached in the ∆ggpS chassis grown under 3% NaCl. Taking into consideration this production under seawater-like salinity, and the identification of main key players involved in the carbon fluxes, this work paves the way for a feasible production of this, or other compatible solutes, using optimized Synechocystis chassis in a pilot-scale.

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Photosynthetic Conversion of Carbon Dioxide to Oleochemicals by Cyanobacteria: Recent Advances and Future Perspectives

Cited by 23 publications

References 118 publications

Long-Chain Saturated Fatty Acids, Palmitic and Stearic Acids, Enhance the Repair of Photosystem II

Long-Chain Saturated Fatty Acids, Palmitic and Stearic Acids, Enhance the Repair of Photosystem II

High-Light-Induced Stress Activates Lipid Deacylation at the Sn-2 Position in the Cyanobacterium Synechocystis Sp. PCC 6803

Heterologous Production of Glycine Betaine Using Synechocystis sp. PCC 6803-Based Chassis Lacking Native Compatible Solutes

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