Photosynthetic characterization of two Nannochloropsis species and its relevance to outdoor cultivation

Vonshak, Avigad; Novoplansky, Nurit; Benavides, Ana Margarita Silva; Torzillo, Giuseppe; Beardall, John; Palacios, Yussi

doi:10.1007/s10811-019-01985-5

Cited by 13 publications

(4 citation statements)

References 35 publications

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“…( 5) and ( 6) respectively). Similar behavior was observed in the photosynthesis parameters elaborated on a chlorophyll and cell basis in Nannochloropsis species exposed to different light intensities [41]. These results indicate an adaptation of the overall photosynthetic apparatus to the different steady-state conditions (light and nutrients regime) which led to different photosynthetic performance.…”

Section: Biomass Production and Photosynthetic Performancesupporting

confidence: 75%

Optimization of the production of lipids and carotenoids in the microalga Golenkinia aff. brevispicula

et al. 2020

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Section: Biomass Production and Photosynthetic Performancesupporting

confidence: 75%

Optimization of the production of lipids and carotenoids in the microalga Golenkinia aff. brevispicula

et al. 2020

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“…Multi-cultivators provide on-line monitoring of optical density at 720 nm (OD(MC) in the following). However, as noted by Vonshak et al [46], we found that this apparent optical density is not linearly related to true optical density, as measured in a conventional spectrophotometer, especially at high cell densities. The relationship between OD(MC) and true optical density (OD(750), measured at 750 nm in a lambda 20 UV/Vis spectrophotometer, Perkin-Elmer) was therefore investigated for correction purposes.…”

Section: Specific Growth Rate and Productivity Determinationsupporting

confidence: 56%

Heterotrophy Compared to Photoautotrophy for Growth Characteristics and Pigment Compositions in Batch Cultures of Four Green Microagae

Le,

Corato,

Gerards

et al. 2023

Preprint

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Four strains of green microalgae (Scenedesmus acutus, Scenedesmus vacuolatus, Chlorella sorokiniana and Chlamydomonas reinhardtii) were compared for growth and pigment composition under photoautotrophic or heterotrophic conditions. Batch growth experiments were performed in multicultivators with online monitoring of optical density. For photoautotrophic growth, light-limited (CO2-sufficient) growth was analysed under different light intensities during exponential and deceleration growth phases. The specific growth rate, mesured during exponential phase, and the maximal biomass productivity, measured during deceleration phase, were not related to each other when different light intensities and different species were considered. This indicates species-dependent photoacclimation effects during cultivation time, which was confirmed by light-dependent changes in pigment contents and compositions when exponential and deceleration phases were compared. Except for C. reinhardtii, that does not grow on glucose, heterotrophic growth was promoted to similar extents by acetate or by glucose, however these two substrates led to different pigment compositions. Weak light increased pigment contents during heterotrophy in the four species, but was efficient in promoting growth only in S. acutus. C. sorokiniana and S. vacuolatus exhibited the best potential for heterotrophic biomass productivities both on glucose and acetate, with carotenoid (lutein) content being the highest in the former.

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“…1 and 2.5 • 10 6 cells ml -1 , Supplementary Figure S2) in Nannochloropsis. These concentration values are on average 30 times lower than those used with alternative Clark sensors (Ben-Sheleg et al, 2021;Perin et al, 2015;Vonshak et al, 2020), minimizing the volume of sample to sacrifice for the evaluation of photosynthetic activity of microalgae cultures. More importantly, the investigation of photosynthetic functionality at low cell concentration opens the possibility to investigate cultures with minimal shading.…”

Section: Estimation Of Pi Relationship In Both Diluted and Dense Micr...mentioning

confidence: 93%

Assessment of photosynthetic activity in dense microalgae cultures using oxygen production

Vera-Vives,

Michelberger,

Morosinotto

et al. 2023

Preprint

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Microalgae are photosynthetic microorganisms playing a pivotal role in primary production in aquatic ecosystems, sustaining the entry of carbon in the biosphere. Microalgae have also been recognized as sustainable source of biomass to complement crops. For this objective they are cultivated in photobioreactors or ponds at high cell density to maximize biomass productivity and lower the cost of downstream processes.Photosynthesis depends on light availability, that is often not constant over time. In nature, sunlight fluctuates over diurnal cycles and weather conditions. In high-density microalgae cultures of photobioreactors outdoors, on top of natural variations, microalgae are subjected to further complexity in light exposure. Because of the high-density cells experience self-shading effects that heavily limit light availability in most of the mass culture volume. This limitation strongly affects biomass productivity of industrial microalgae cultivation plants with important implication on economic feasibility.Understanding how photosynthesis responds to cell density is informative to assess functionality in the inhomogeneous light environment of industrial photobioreactors. In this work we exploited a high-sensitivity Clark electrode to measure microalgae photosynthesis and compare cultures with different densities, usingNannochloropsisas model organism. We observed that cell density has a substantial impact on photosynthetic activity, and demonstrated the reduction of the cell’s light-absorption capacity by genetic modification is a valuable strategy to increase photosynthetic functionality of dense microalgae cultures.HighlightsMicroalgae biomass is a promising alternative to crops.The impact of cultivation at scale on photosynthesis is still under-investigated.The Photosynthesis-Irradiance (PI) relationship is informative.High-sensitivity oxygen measurements for PI investigation was validated.The effect of cell density on PI was studied inNannochloropsispale mutants.

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Photosynthetic characterization of two Nannochloropsis species and its relevance to outdoor cultivation

Cited by 13 publications

References 35 publications

Optimization of the production of lipids and carotenoids in the microalga Golenkinia aff. brevispicula

Optimization of the production of lipids and carotenoids in the microalga Golenkinia aff. brevispicula

Heterotrophy Compared to Photoautotrophy for Growth Characteristics and Pigment Compositions in Batch Cultures of Four Green Microagae

Assessment of photosynthetic activity in dense microalgae cultures using oxygen production

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