Photosynthetic characterization and expression profiles of sugarcane infected by Sugarcane mosaic virus (SCMV)

Abstract

Sugarcane mosaic virus (SCMV), belonging to genus Potyvirus, family Potyviridae, is a severe pathogen of several agricultural important crops, mainly sugarcane. Due to complex nature of sugarcane, the effect of SCMV pathogenicity on sugarcane photosynthetic systems remains to be explored. In this study, we investigated the alterations occurring in the photosynthetic system in the sugarcane genotypes at the cytopathological, physiological and biological, transcriptome and proteome level. We generated the transcriptome assembly of two genotypes (susceptible Badila and resistant B-48) using Saccharum spontaneum L. as a reference genome. RNA-sequencing data revealed the significant upregulation of NAD(P) H, RubisCO, oxygen-evolving complex, chlorophyll a and b binding protein, Psb protein family, PSI reaction center subunit II, and IVgenes in B-48, as compared to its counterparts. Upregulated genes in B-48 are associated with various processes such as stability and assembly of photosystem, protection against photoinhibition and antiviral defense. The expression pattern of differentially abundant genes were further verified at the proteomics level. Overall, differentially expressed genes/ proteins (DEGs/DEPs) showed the consistency of expression at both transcriptome and proteome level in B-48 genotype. Comprehensively, these data supported the efficiency of B-48 genotype under virus infection conditions and provided a better understanding of the expression pattern of photosynthesis-related genes in sugarcane.

Incidence and detection of wilting pathogen and Sugarcane mosaic virus (SCMV) in Pakistan

“…Sugarcane yellow leaf disease (SYLD) and sugarcane mosaic disease (SMD) are the most reported viral diseases, so far. Sugarcane streak mosaic virus (SCSMV, unassigned genus, family Potyviridae ), Sorghum mosaic virus (SrMV, genus Potyvirus , family Potyviridae ) and Sugarcane mosaic virus (SCMV, genus Potyvirus , family Potyviridae ) have been reported to cause SMD (Akbar et al, 2021). SCMV has been reported to be distributed, worldwide (Gemechu et al, 2006), whereas SrMV is mainly found in China, United States and Vietnam (Ha et al, 2008).…”

“…Typical symptoms of wilt disease include leaf wilting, yellowing, restricted growth, red discoloration of the xylem vessels to stem or root decay (Okungbowa & Shittu, 2012). to cause SMD (Akbar et al, 2021). SCMV has been reported to be distributed, worldwide (Gemechu et al, 2006), whereas SrMV is mainly found in China, United States and Vietnam (Ha et al, 2008).…”

Selective Interaction of Sugarcane eIF4E with VPgs from Sugarcane Mosaic Pathogens

“…is the most important sugar and energy crop worldwide [ 1 , 2 , 3 ]. Sugarcane mosaic disease is widespread in sugarcane-growing countries [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ] and causes heavy yield losses [ 16 , 17 ]. In the 1920s, sugarcane mosaic disease almost collapsed the sugar industry in Argentina, Brazil, and Louisiana [ 18 ].…”

“…In the 1920s, sugarcane mosaic disease almost collapsed the sugar industry in Argentina, Brazil, and Louisiana [ 18 ]. Although sugarcane mosaic disease is controlled by planting resistant sugarcane cultivars, it continues to be a threat to the sugarcane industry [ 4 , 15 , 17 , 19 ]. The main causal agents for the sugarcane mosaic disease are Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), and Sugarcane streak mosaic virus (SCSMV), which belong to the Potyviridae family, with SCMV and SrMV being members of the Potyvirus genus [ 20 ], whereas SCSMV is a member of Poacevirus genus [ 21 , 22 , 23 ].…”

“…The molecular mechanism of sugarcane mosaic pathogen infection in sugarcane is poorly understood [ 15 , 17 , 30 , 57 , 58 , 59 ] due to the highly complex sugarcane genome and difficulty of performing transformation [ 59 , 60 ]. In the present study, we cloned 4 eIF4E homologs obtained from sugarcane cultivar ROC22 and designated them as SceIF4Ea , SceIF4Eb , SceIF(iso)4E , and ScnCBP .…”