Photosynthetic and physiological analysis of the rice high-chlorophyll mutant (Gc)

Kang, Zhenhui; Li, Guanrong; Huang, Junli; Niu, Xiaodong; Zou, Hanyan; Zang, Guangchao; Wenwen, Yihao; Wang, Guixue

doi:10.1016/j.plaphy.2012.07.019

Cited by 13 publications

(5 citation statements)

References 35 publications

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“…The increased chlorophyll content was suggested to result from the AMFinduced increase in the uptake of nutrients, espe-cially magnesium, which is an important component of chlorophyll 31 and increase the levels of core proteins of photosystem I and II and the lightharvesting complex II in Indica rice, leading to increased photosynthetic capacity 32,33 . The higher available phosphorus content in AMF-inoculated soil was suggested as another important factor in increasing photosynthesis in lettuce, by increasing the amount of ATP available to fuel CO 2 assimilation in the Calvin cycle 34 .…”

Section: Discussionmentioning

confidence: 99%

Arbuscular mycorrhizal fungus improves the yield and quality of Lactuca sativa in an organic farming system

Charoonnart¹,

Seraypheap²,

Chadchawan³

et al. 2016

ScienceAsia

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Arbuscular mycorrhizal fungi have been reported to enhance the growth of many crop plants. This study examines the effect of Funneliformis mosseae, a type of arbuscular mycorrhizal fungus (AMF), on the growth of butterhead lettuce in organic cultivation. Lettuces were inoculated with 50 spores of F. mosseae to determine their physiological and biochemical characters compared to uninoculated lettuces in an abandoned field. After 60 days of growth, the AMF-inoculated plants had significantly more leaves (on average, 19.4) and greater leaf fresh weight (on average, 30.4 g) than for the no-AMF treatment which had 15.8 leaves with a total weight of 14.5 g. Net photosynthesis and the chlorophyll a content were higher in the AMF-inoculated plants than in the control plants. The rhizosphere soil of 60-day-old AMF-inoculated butterhead lettuce showed higher organic matter, available phosphorus content, and exchangeable potassium content. The plants in the AMF treatment showed higher total nitrogen, phosphorus, and potassium contents in leaf and root tissues than the control plants. Antioxidant capacity was analysed by quantifying catalase and ascorbate peroxidase activities, ascorbic acid content, and carotenoid content. All of these parameters tended to be higher in the AMF treatment than in the control. The diphenylpicrylhydrazyl radical scavenging activity in AMF-inoculated plants (54%) was twice that in the control plants (24%). These results demonstrate that applying AMF is a cost-effective way to enhance the growth of organically farmed butterhead lettuce.

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Section: Discussionmentioning

confidence: 99%

Arbuscular mycorrhizal fungus improves the yield and quality of Lactuca sativa in an organic farming system

Charoonnart¹,

Seraypheap²,

Chadchawan³

et al. 2016

ScienceAsia

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“…Thylakoid membrane was isolated from mid-veins and leaf laminae as described earlier (Kang et al 2012 ). The Chl content was determined spectrophotometrically in 80 % acetone (Arnon 1949 ).…”

Section: Methodsmentioning

confidence: 99%

The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice

Shen

et al. 2016

Rice

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BackgroundRecent studies have shown that C4-like photosynthetic pathways partly reside in photosynthetic cells surrounding the vascular system of C3 dicots. However, it is still unclear whether this is the case in C3 monocots, especially at the molecular level.ResultsIn order to fill this gap, we investigated several characteristics required for C4 photosynthesis, including C4 pathway enzymes, cyclic/non-cyclic photophosphorylation rates, the levels and assembly state of photosynthetic machineries, in the mid-veins of C3 monocots rice with leaf laminae used as controls. The signature of photosystem photochemistry was also recorded via non-invasive chlorophyll a fluorescence and reflectance changes at 820 nm in vivo. Our results showed that rice mid-veins were photosynthetically active with higher levels of three C4 decarboxylases. Meanwhile, the linear electron transport chain was blocked in mid-veins due to the selective loss of dysfunctional photosystem II subunits. However, photosystem I was sufficient to support cyclic electron flow in mid-veins, reminiscent of the bundle sheath in C4 plants.ConclusionsThe photosynthetic attributes required for C4 photosynthesis were identified for the first time in the monocotyledon model crop rice, suggesting that this is likely a general innate characteristic of C3 plants which might be preconditioned for the C4 pathway evolution. Understanding these attributes would provide a base for improved strategies for engineering C4 photosynthetic pathways into rice.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-016-0094-5) contains supplementary material, which is available to authorized users.

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“…Isolation of thylakoid membrane complexes and total soluble protein: Thylakoid membrane complexes were isolated from the mid-portion of the rice leaves as described earlier (Kang et al 2012). The Chl content was determined spectrophotometrically (Cintra 1010, GBC scientific equipment, Australia) in 80% acetone (Arnon 1949).…”

Section: Relative Water Content (Rwc) and Chlorophyll (Chl) Contentmentioning

confidence: 99%

Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane

Shen¹,

Chen²,

Xu³

et al. 2015

Photosynt.

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Drought impacts severely crop photosynthesis and productivity. Development of transgenic rice overexpressing maize phosphoenolpyruvate carboxylase (PEPC) is a promising strategy for improving crop production under drought stress. However, the molecular mechanisms of protection from PEPC are not yet clear. The objective of this study was: first, to characterize the response of individual photosynthetic components to drought stress; second, to study the physiological and molecular mechanisms underlying the drought tolerance of transgenic rice (cv. Kitaake) over-expressing maize PEPC. Our results showed that PEPC overexpressing improved the ability of transgenic rice to conserve water and pigments during drying as compared to wild type. Despite the fact that drought induced reactive oxygen species and damaged photosystems (especially, PSI) in both lines, higher intercellular CO2 concentration protected the photosynthetic complexes, peptides, and also ultrastructure of thylakoid membranes against the oxidative damage in transgenic rice. In conclusion, although photosynthetic apparatus suffered an inevitable and asymmetric impairment during drought conditions, PEPC effectively alleviated the oxidative damage on photosystems and enhanced the drought tolerance by increasing intercellular CO 2 concentration. Our investigation provided critical clues for exploring the feasibility of using C 4 photosynthesis to increase the yield of rice under the aggravated global warming.

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Photosynthetic and physiological analysis of the rice high-chlorophyll mutant (Gc)

Cited by 13 publications

References 35 publications

Arbuscular mycorrhizal fungus improves the yield and quality of Lactuca sativa in an organic farming system

Arbuscular mycorrhizal fungus improves the yield and quality of Lactuca sativa in an organic farming system

The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice

Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane

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