Photosynthesis-related physiology and metabolomics responses of

Abstract

Manganese (Mn) plays an essential role in plant growth; however, excessive Mn is toxic to plants. Polygonum lapathifolium Linn. was tested as a novel Mn-hyperaccumulating species in our previous study, but the underlying mechanisms of this hyperaccumulation are poorly understood. A hydroponic experiment with (8 mmol L−1) and without additional Mn (CK) was established to explore the possible mechanisms through the effects on photosynthesis-related physiological characteristics and metabolomics. The results showed that additional Mn increased plant biomass, photosynthesis, and stomatal conductance related to increases in the effective photochemical quantum yield of photosystem II and relative electron transport rate (P < 0.05). The results from liquid chromatography–mass spectrometry revealed 56 metabolites differentially accumulated between the plants composing these two groups. Metabolites were enriched in 20 metabolic pathways at three levels (environmental information processing, genetic information processing, and metabolism), of which five metabolic pathways were associated with significant or extremely significant changes (P < 0.05). These five enriched pathways were ABC transporters (environmental information processing), aminoacyl-tRNA biosynthesis (genetic information processing), biosynthesis of amino acids, d-arginine and d-ornithine metabolism, and arginine biosynthesis (metabolism). Flavonoids may play a key role in Mn tolerance, as they accumulated more than 490-fold, and the relationship between flavonoids and Mn tolerance needs to be studied in the future.

Manganese sulfate application promotes berry flavonoid accumulation in Vitis vinifera cv. ‘Cabernet Sauvignon’ by regulating flavonoid metabolome and transcriptome profiles

“…In addition, Abscisic Acid (ABA) and total phenolic concentrations were significantly increased in table grape leaves under Mn treatment, which was consistent with the oxidative stress resistance of Mn‐tolerant table grapes 2,23 . Recently, Mn treatment was also reported to increase the flavonoid content in wheat and Polygonum lapathifolium 1,38 . Our findings further support the high concentration of flavonoids in plants under H treatment, which may be because flavonoids protect plants from oxidative damage by chelating metals to prevent free radical production under heavy metal stress 39 …”

“…2,23 Recently, Mn treatment was also reported to increase the flavonoid content in wheat and Polygonum lapathifolium. 1,38 Our findings further support the high concentration of flavonoids in plants under H treatment, which may be because flavonoids protect plants from oxidative damage by chelating metals to prevent free radical production under heavy metal stress. 39 Transcriptome analysis of manganese-treated grapes The samples produced 178.04 Gb of clean data, with each sample providing more than 6.31 Gb, and the Q30 being higher than 89%.…”

“…Manganese (Mn) is regarded as one of the most important micronutrient elements in plant life processes, especially for fruit development. It is involved directly in the scavenging of reactive oxygen species (ROS), in photosynthesis, in flavonoid metabolism, and in one of the main pathways for polyphenol synthesis 1 . The supply of exogenous Mn promotes phenylpropanoid metabolism and the accumulation of phenolic compounds in grape leaves, and improves powdery mildew resistance by triggering the oxidation defense system 2 .…”

“…It is involved directly in the scavenging of reactive oxygen species (ROS), in photosynthesis, in flavonoid metabolism, and in one of the main pathways for polyphenol synthesis. 1 The supply of exogenous Mn promotes phenylpropanoid metabolism and the accumulation of phenolic compounds in grape leaves, and improves powdery mildew resistance by triggering the oxidation defense system. 2 Photosynthetic systems also require large amounts of Mn, mainly for Mn 4 Ca-cluster formation in the oxygen-evolving complex (OEC) of photosystem II (PSII), which is needed for water-splitting reactions and the maintenance of the chloroplast structure.…”

Does sulfate improve the Mn bioremediation efficiency of Polygonum lapathifolium Linn?

“…On the 55th day of growth, the gas exchange parameters, including the photosynthesis rate (Pn), transpiration rate (Tr), gas stomatal conductance (Gs), and intercellular CO 2 concentration (Ci), were determined with an LI-6800XT instrument (LI-6800XT, LI-COR, Corp, USA) (Wang et al 2023). For each pot, each plant with one fully expanded mature leaf was selected for gas exchange parameter measurement.…”