PHOTOSYNTHESIS <i>IN VIVO</i> CAN BE LIMITED BY PHOSPHATE SUPPLY

Sivak, Mirta N.; Walker, David A.

doi:10.1111/j.1469-8137.1986.tb00826.x

Cited by 163 publications

(100 citation statements)

References 44 publications

(21 reference statements)

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“…The P, at saturating COz and light is usually limited by the rate of phosphate liberation from Suc and starch synthesis pathways (Laisk and Laarin, 1983;Sivak and Walker, 1986). The much lower P, of the mutant that lacks starch synthesis agrees well with this understanding (Sivak and Rowell, 1988).…”

Section: Externa1 Pi Feedingsupporting

confidence: 68%

CO2 Uptake and Electron Transport Rates in Wild-Type and a Starchless Mutant of Nicotiana sylvestris (The Role and Regulation of Starch Synthesis at Saturating CO2 Concentrations)

Eichelmann

Laisk

1994

Plant Physiol.

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CO, uptake rate, chlorophyll fluorescence, and 830-nm absorbance were measured in wild-type (wt) Nicotiana sylvestris (Speg.et Comes) and starchless mutant NS 458 leaves at different light intensities and CO, concentrations. lnitial slopes of the relationships between CO, uptake and light and CO, were similar, but the maximum rate at CO, and light saturation was only 30% in the mutant compared with the wt. O , enhancement of photosynthesis at CO, and light saturation was relatively much greater in the mutant than in the wt. In 21% O,, the eledron transport rate (ETR) calculated from fluorescence peaked near the beginning of the CO, saturation of photosynthesis. With the further increase of CO, concentration ETR remained nearly constant or declined a little in the wt but drastically declined in the mutant. Absorbance measurements at 830 nm indicated photosystem I acceptor side reduction in both plants at saturating CO, and light. Assimilatory charge (postillumination CO, uptake) measurements indicated trapping of chloroplast inorganic phosphate, supposedly in hexose phosphates, in the mutant. It is concluded that starch synthesis gradually substitutes for photorespiration as electron acceptor with increasing CO, concentration in the wt but not in the mutant. It is suggested that starch synthesis is co-controlled by the activity of the chloroplast fructose bisphosphatase.Transgenic and mutant plants are widely used in studies of metabolic control. Plants deficient in some starch-synthesis enzyme have been used for investigating the partitioning of carbon between Suc and starch and the control of starch synthesis. Examples are Arabidopsis thaliana with varied expression of ADP-Glc pyrophosphorylase and plastid phosphoglucomutase , Clarkia xantiana with reduced activity of Glc-P isomerase in the cytosol and chloroplast (Kruckeberg et al., 1988), and Nicotiana sylvestris with modified plastid phosphoglucomutase (Hanson and McHale, 1988). The latter has been investigated by using gasexchange and Chl-fluorescence methods (Hanson, 1990a(Hanson, , 1990bPeterson and Hanson, 1991). C02-fixation rates of the wt and mutant tobacco differed little at limiting C 0 2 concentrations, but the C02-exchange rate in the mutant was about half of that in the wt at C 0 2 and light saturation at 3OOC. The lower rates of maximal CO, assimilation in the 679mutant were not due to gross differences in stomatal conductance or in levels of enzymes of the Calvin cycle but, rather, were due to limitations in the ability to regenerate RuBP that was mediated by the Pi released during Suc synthesis. A good correlation was obtained between the values of the intrinsic quantum yield of photosynthesis calculated from fluorescence data and that calculated from gasexchange data.In this work we continued the studies of the electron transport and gas exchange in the wt and starchless mutant NS 458 of N. sylvestris. Our aim was to understand the role and regulation of starch synthesis as electron acceptor in photosynthesis. We have measured the COz dep...

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Section: Externa1 Pi Feedingsupporting

confidence: 68%

CO2 Uptake and Electron Transport Rates in Wild-Type and a Starchless Mutant of Nicotiana sylvestris (The Role and Regulation of Starch Synthesis at Saturating CO2 Concentrations)

Eichelmann

Laisk

1994

Plant Physiol.

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“…constituent of proteins and it determines the photosynthetic capacity (Güsewell, 2004), as up to 75% of N would be involved in photosynthetic processes (Evans, 1989). Although P is an important structural element in nucleic acids that regulate energy storage and transfer (Wang et al, 2013) and photosynthesis requires orthophosphate as a substrate (Sivak and Walker, 1986), the pool of phosphorus involved in photosynthesis is variable and often small (Bieleski, 1973). Thus, an efficient use of N and P is believed to contribute to plant fitness (Aerts and Chapin, 2000) and is thought to influence the nutrient cycling and productivity of terrestrial ecosystems (Vitousek, 2004).…”

Section: Photosynthesis and Photosynthetic Element Use Efficiency Of mentioning

confidence: 99%

Effects of nitrogen and phosphorus supply on growth and physiological traits of two Larix species

Guo

Zhang

et al. 2016

Environmental and Experimental Botany

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“…Phosphorus deficiency limits whole-plant photosynthesis (Heineke, Stitt & Heldt 1989) and, as a consequence, growth is reduced (Sivak & Walker 1986). Despite the dramatic effects of low-phosphorus supply on plant size, specific rates of photosynthesis may (Jacob & Lawlor 1993;Rao, Fredeen & Terry 1993) or may not (Foyer & Spencer 1986;Kondracka & Rychter 1997) be affected.…”

mentioning

confidence: 99%

Metabolic regulation of leaf respiration and alternative pathway activity in response to phosphate supply

et al. 2001

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In this study the question whether the alternative respiratory pathway acts as an electron bypass for the cytochrome pathway under conditions of growth on limited phosphorus in leaves of bean (Phaseolus vulgaris L.), tobacco (Nicotiana tabacum L.) and Gliricidia sepium Walp was investigated. The oxygen isotope fractionation technique was used to assess the in vivo activities of the cytochrome and alternative respiratory pathways in the absence of added inhibitors. The response of respiration to low phosphorus supply varied among species. Growth at low phosphorus reduced cytochrome pathway activity in bean and tobacco. Alternative pathway activity increased only in bean leaves in response to low phosphorus and not in tobacco. In the case of G. sepium, cytochrome pathway activity remained unchanged whereas the alternative pathway activity increased with low nutritional phosphorus. At low phosphorus, alternative oxidase protein levels increased in the leaves of bean and G. sepium but not in tobacco, suggesting a dependence of alternative pathway activity on protein level. Alternative pathway activity was also not correlated with soluble carbohydrate concentration in bean or tobacco at any phosphorus level. These results show that the alternative pathway does not always act as an electron bypass in response to the downstream restriction of the cytochrome pathway imposed by low phosphorus supply. These results suggest that factors in addition to cellular carbohydrate level and adenylate control can act to regulate alternative pathway activity.

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PHOTOSYNTHESIS IN VIVO CAN BE LIMITED BY PHOSPHATE SUPPLY

Cited by 163 publications

References 44 publications

CO2 Uptake and Electron Transport Rates in Wild-Type and a Starchless Mutant of Nicotiana sylvestris (The Role and Regulation of Starch Synthesis at Saturating CO2 Concentrations)

CO2 Uptake and Electron Transport Rates in Wild-Type and a Starchless Mutant of Nicotiana sylvestris (The Role and Regulation of Starch Synthesis at Saturating CO2 Concentrations)

Effects of nitrogen and phosphorus supply on growth and physiological traits of two Larix species

Metabolic regulation of leaf respiration and alternative pathway activity in response to phosphate supply

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