Photosynthesis does influence superoxide production in the ichthyotoxic alga Chattonella marina (Raphidophyceae)

Marshall, Judith-Anne; Hovenden, Mark J.; Oda, Tatsuya; Hallegraeff, Gustaaf M.

doi:10.1093/plankt/24.11.1231

Cited by 80 publications

(84 citation statements)

References 19 publications

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“…Robbins et al (64) hinted at this when, on glass slides submerged in a Mn-contaminated creek, they observed that some, but not all, diatoms were coated with Mn oxides, whereas all would be expected to produce Mn oxides if the mechanism were purely photosynthesis-linked pH modulation. Many algae are known to produce reactive oxygen species, notably superoxide, as a by-product of photosynthesis, as antibacterial agents (66), and in response to metal contamination (59). Some bacteria (25) and fungi (22) oxidize Mn(II) via superoxide production, and it is possible that some of the algae in our MRBs can do the same.…”

Section: Discussionmentioning

confidence: 99%

Profiling Microbial Communities in Manganese Remediation Systems Treating Coal Mine Drainage

Chaput

Hansel

Burgos

et al. 2015

Appl Environ Microbiol

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cWater discharging from abandoned coal mines can contain extremely high manganese levels. Removing this metal is an ongoing challenge. Passive Mn(II) removal beds (MRBs) contain microorganisms that oxidize soluble Mn(II) to insoluble Mn(III/IV) minerals, but system performance is unpredictable. Using amplicon pyrosequencing, we profiled the bacterial, fungal, algal, and archaeal communities in four MRBs, performing at different levels, in Pennsylvania to determine whether they differed among MRBs and from surrounding soil and to establish the relative abundance of known Mn(II) oxidizers. Archaea were not detected; PCRs with archaeal primers returned only nontarget bacterial sequences. Fungal taxonomic profiles differed starkly between sites that remove the majority of influent Mn and those that do not, with the former being dominated by Ascomycota (mostly Dothideomycetes) and the latter by Basidiomycota (almost entirely Agaricomycetes). Taxonomic profiles for the other groups did not differ significantly between MRBs, but operational taxonomic unit-based analyses showed significant clustering by MRB with all three groups (P < 0.05). Soil samples clustered separately from MRBs in all groups except fungi, whose soil samples clustered loosely with their respective MRB. Known Mn(II) oxidizers accounted for a minor proportion of bacterial sequences (up to 0.20%) but a greater proportion of fungal sequences (up to 14.78%). MRB communities are more diverse than previously thought, and more organisms may be capable of Mn(II) oxidation than are currently known.

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Section: Discussionmentioning

confidence: 99%

Profiling Microbial Communities in Manganese Remediation Systems Treating Coal Mine Drainage

Chaput

Hansel

Burgos

et al. 2015

Appl Environ Microbiol

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“…7). It has been shown that ROS production in C. marina was significantly reduced with 24 h of darkness (Marshall et al, 2001), or 5 h after DCMU treatment (an inhibitor of photosynthesis) (Marshall et al, 2002). However, measurements in both of these experiments were conducted not long after but not immediately following exposure, and no measurements were made of energy storage and energy metabolism (e.g.…”

Section: Physical Parameters and Rosmentioning

confidence: 99%

“…Chattonella marina, a harmful algal bloom (HAB) species, is able to produce reactive oxygen species (ROS, including superoxide anion radicals, O 2 ⎯• , hydrogen peroxide, H 2 O 2 , and hydroxyl radicals, OH · ) (Oda et al, 1998) at levels 100 times higher than those produced by most algal species (Marshall et al 2002). In photosynthetic organisms, ROS are continuously produced as byproducts through various metabolic pathways localized in mitochondria, chloroplasts, and peroxisomes (Apel and Hirt, 2004).…”

Section: Introductionmentioning

confidence: 99%

Effects of nutrients, salinity, pH and light:dark cycle on the production of reactive oxygen species in the alga Chattonella marina

Wenhua

Anderson

et al. 2007

Journal of Experimental Marine Biology and Ecology

128

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Experiments were carried out to investigate the effects of nutrients, salinity, pH and light:dark cycle on growth rate and production of reactive oxygen species (ROS) by Chattonella marina, a harmful algal bloom (HAB) species that often causes fish kills. Different nitrogen forms (organic-N and inorganic-N), N:P ratios, light:dark cycles and salinity significantly influenced algal growth, but not ROS production.However, iron concentration and pH significantly affected both growth and ROS production in C. marina. KCN (an inhibitor of mitochondrial respiration) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (an inhibitor of photosynthesis) had no significant effects on ROS production. Vitamin K 3 (a plasma membrane electron shuttle) enhanced ROS production while its antagonist, dicumarol, decreased ROS production. Taken together, our results suggest that ROS production by C. marina is related to a plasma membrane enzyme system regulated by iron availability but is independent of growth, photosynthesis, availability of macronutrients, salinity and irradiance.

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“…The mechanism of ichthyotoxicity of C. antiqua is not known in detail, but Chattonella spp. generate reactive oxygen species (ROS) such as the superoxide anion (O 2 -) and hydrogen peroxide (H 2 O 2 ) (Shimada et al 1991, 1993, Oda et al 1992, Tanaka et al 1992, 1994, Marshall et al 2002. Since ROS are generally toxic to living organisms (Oda et al 1989(Oda et al , 1997, those generated by Chattonella spp.…”

Section: Introductionmentioning

confidence: 99%

Changes in O2- and H2O2 production by Chattonella antiqua during diel vertical migration under nutrient stratification

Kim¹,

Watanabe²,

Nakayasu³

et al. 2005

Aquat. Microb. Ecol.

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The red tide flagellate Chattonella antiqua produces high levels of reactive oxygen species (ROS) such as the superoxide anion (O 2 -) and hydrogen peroxide (H 2 O 2 ). Production of ROS by this flagellate species depends on nutrient and water conditions during its growth phases. To clarify the effect of nutrient depletion on ROS production, we investigated the production pattern of ROS in a culture tank containing 1 m 3 of medium with low nitrate and phosphate nutrient concentrations. The production of O 2 -and H 2 O 2 by C. antiqua was lower when nutrients were limited than when they were fully supplied (f/2 medium), as indicated by reductions in nitrogen and phosphorus cell quotas. We also examined ROS formation by C. antiqua under stratified conditions subsequent to the nutrient depletion experiment. The vertical stratification of salinity (∆S = 2.5 psu) and temperature (∆T = 5°C) in the tank were analogous to natural conditions during red tides, and only the bottom layer was enriched with nutrients. C. antiqua showed clear diel vertical migration under stratified conditions. The ROS production due to photosynthesis was high during the light period at the surface, even though the ambient nutrient concentration of the surface was low, and it decreased in the dark bottom layer. The generation of H 2 O 2 increased continuously for 4 d, but O 2 -generation peaked on the second day of stratification and then gradually decreased. KEY WORDS: Chattonella antiqua · Diel vertical migration · Hydrogen peroxide · Nutrient stratification · Reactive oxygen species · Superoxide anion Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 39: [183][184][185][186][187][188][189][190][191] 2005 division occurred almost once per day during the exponential growth phase (Kim et al. 2004).Chattonella antiqua is a motile phytoplankton characterized by diel vertical migration (DVM) and nocturnal nutrient uptake . The cells accumulate at the surface during the day for photosynthesis and sink to a deeper layer, where nutrients such as nitrate and phosphate are abundant, to take up the nutrients during the night. Under stratified conditions, the DVM ability gives the cells an ecological advantage over other non-motile phytoplankton species because the surface nutrients are generally almost depleted. The DVM of the flagellate population was examined in a culture tank at the National Institute for Environmental Studies (NIES), Japan (Kohata & Watanabe 1986, 1987, Kimura et al. 1999, in terms of nutrition and growth. Kim et al. (2004) suggested that the toxicity induced by ROS was presumably linked to cell migration through the cell cycle and nutrition although their study on ROS generation by C. antiqua was conducted in the NIES tank under fully mixed conditionsFor the present study, we grew Chattonella antiqua in the NIES culture tank under limited nitrate and phosphate nutrient conditions to clarify the effect of nutrient depletion on ROS production through the growth metabolism du...

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Photosynthesis does influence superoxide production in the ichthyotoxic alga Chattonella marina (Raphidophyceae)

Cited by 80 publications

References 19 publications

Profiling Microbial Communities in Manganese Remediation Systems Treating Coal Mine Drainage

Profiling Microbial Communities in Manganese Remediation Systems Treating Coal Mine Drainage

Effects of nutrients, salinity, pH and light:dark cycle on the production of reactive oxygen species in the alga Chattonella marina

Changes in O2- and H2O2 production by Chattonella antiqua during diel vertical migration under nutrient stratification

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