Photosynthesis, Carbohydrate Metabolism, and Yield of Phytochrome‐B‐Overexpressing Potatoes under Different Light Regimes

Schittenhelm, Siegfried; Menge-Hartmann, Ute; Oldenburg, Elisabeth

doi:10.2135/cropsci2004.1310

Cited by 39 publications

(21 citation statements)

References 35 publications

(37 reference statements)

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“…These findings suggest that phyB plays a key role in the systemic effects on the induction of photosynthesis. In agreement with these observations, phyB mutants show decreased CO 2 assimilation rates (Boccalandro et al, 2009), and conversely, overexpression of PHYB increased CO 2 assimilation rates compared with wildtype plants (Schittenhelm et al, 2004). Taken together, these results demonstrate that R light received by phyB at the shoot apex is the initial trigger for systemic signaling to the distal leaves.…”

Section: Discussionsupporting

confidence: 83%

Systemic induction of photosynthesis via illumination of the shoot apex is mediated by phytochrome B

et al. 2016

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Systemic signaling of upper leaves promotes the induction of photosynthesis in lower leaves, allowing more efficient use of light flecks. However, the nature of the systemic signals has remained elusive. Here, we show that preillumination of the tomato (Solanum lycopersicum) shoot apex alone can accelerate photosynthetic induction in distal leaves and that this process is light quality dependent, where red light promotes and far-red light delays photosynthetic induction. Grafting the wild-type rootstock with a phytochome B (phyB) mutant scion compromised light-induced photosynthetic induction as well as auxin biosynthesis in the shoot apex, auxin signaling, and RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1)-dependent hydrogen peroxide (H 2 O 2 ) production in the systemic leaves. Light-induced systemic H 2 O 2 production in the leaves of the rootstock also was absent in plants grafted with an auxin-resistant diageotropica (dgt) mutant scion. Cyclic electron flow around photosystem I and associated ATP production were increased in the systemic leaves by exposure of the apex to red light. This enhancement was compromised in the systemic leaves of the wild-type rootstock with phyB and dgt mutant scions and also in RBOH1-RNA interference leaves with the wild type as scion. Silencing of ORANGE RIPENING, which encodes NAD(P)H dehydrogenase, compromised the systemic induction of photosynthesis. Taken together, these results demonstrate that exposure to red light triggers phyB-mediated auxin synthesis in the apex, leading to H 2 O 2 generation in systemic leaves. Enhanced H 2 O 2 levels in turn activate cyclic electron flow and ATP production, leading to a faster induction of photosynthetic CO 2 assimilation in the systemic leaves, allowing plants better adaptation to the changing light environment.

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Section: Discussionsupporting

confidence: 83%

Systemic induction of photosynthesis via illumination of the shoot apex is mediated by phytochrome B

et al. 2016

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“…Thiele et al (1999) determined the semi-dwarfism, decrease in apical dominance, smaller but a higher number of thicker leaves, and increase in pigmentation in PHYB overexpressing potato plants. Schittenhelm et al (2004) determined enhanced photosynthesis in DARA5 leaves, which increased the yield of potato in high irradiation. PHYA and PHYB transgenic lines also showed overexpression in dark grown seedling.…”

Section: Introductionmentioning

confidence: 94%

“…A lot of work has been done on transformation of PHYB in potato (Thiele et al, 1999;Boccalendo et al, 2003;Schittenhelm et al, 2004), tomato (Said et al, 2007), and Arabidopsis (Wagner et al, 1991). The present investigation was carried out in cotton to determine the effect of overexpression of Arabidopsis thaliana PHYB gene on cotton growth and yield.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)

Rao

Irfan

Saleem

et al. 2011

J. Zhejiang Univ. Sci. B

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Abstract:The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens. Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence. Messenger RNA (mRNA) expression in one of the transgenic lines, QCC11, was much higher than those of control and other transgenic lines. Transgenic cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance. The increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate. Data recorded up to two generations, both in the greenhouse and in the field, revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth, with 35% more yield. Moreover, the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism, decrease in apical dominance, and increase in boll size.

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“…Transgenic plants of potato (Solanum tuberosum) expressing the PHYB gene of Arabidopsis show higher stomatal conductance, transpiration rates, and photosynthesis rates per unit leaf area than the wild type (Thiele et al, 1999;Boccalandro et al, 2003;Schittenhelm et al, 2004). Stomata density is unaffected, indicating that phyB enhances the aperture of the stomatal pore in these transgenic plants.…”

mentioning

confidence: 99%

Phytochrome B Enhances Photosynthesis at the Expense of Water-Use Efficiency in Arabidopsis

et al. 2009

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In open places, plants are exposed to higher fluence rates of photosynthetically active radiation and to higher red to far-red ratios than under the shade of neighbor plants. High fluence rates are known to increase stomata density. Here we show that high, compared to low, red to far-red ratios also increase stomata density in Arabidopsis (Arabidopsis thaliana). High red to farred ratios increase the proportion of phytochrome B (phyB) in its active form and the phyB mutant exhibited a constitutively low stomata density. phyB increased the stomata index (the ratio between stomata and epidermal cells number) and the level of anphistomy (by increasing stomata density more intensively in the adaxial than in the abaxial face). phyB promoted the expression of FAMA and TOO MANY MOUTHS genes involved in the regulation of stomata development in young leaves. Increased stomata density resulted in increased transpiration per unit leaf area. However, phyB promoted photosynthesis rates only at high fluence rates of photosynthetically active radiation. In accordance to these observations, phyB reduced long-term water-use efficiency estimated by the analysis of isotopic discrimination against 13 CO 2 . We propose a model where active phyB promotes stomata differentiation in open places, allowing plants to take advantage of the higher irradiances at the expense of a reduction of water-use efficiency, which is compensated by a reduced leaf area.

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Photosynthesis, Carbohydrate Metabolism, and Yield of Phytochrome‐B‐Overexpressing Potatoes under Different Light Regimes

Cited by 39 publications

References 35 publications

Systemic induction of photosynthesis via illumination of the shoot apex is mediated by phytochrome B

Systemic induction of photosynthesis via illumination of the shoot apex is mediated by phytochrome B

Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)

Phytochrome B Enhances Photosynthesis at the Expense of Water-Use Efficiency in Arabidopsis

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