Photosynthesis by isolated chloroplasts.

Abstract

Photosynthesis in green plants requires not only photoelectron transport, oxygen evolution, and photophosphorylation, but also the synthetic reactions whereby carbon dioxide is assimilated and reduced to a number of organic compounds via the photosynthetic carbon reduction cycle1 and secondary biosynthetic pathways.2 Complete photosynthesis with isolated chloroplasts has for many years been a goal of biochemists. Photosynthetic reactions could then be isolated from reactions of the cytoplasm, and the cell wall could be eliminated as a barrier to the assimilation of various added metabolites and chemicals. This achievement would greatly facilitate the study of the mechanisms of enzymic transformations and metabolic control in the synthetic reactions of photosynthesis.The photochemical transfer of electrons from water to artificial electron acceptors by subcellular particles from green plant cells was demonstrated many years ago by Hill and Scarisbrick.3 Since that time, isolated chloroplasts and chloroplast particles have been found to be capable of photoelectron transport to NADP,4-6 and of photophosphorylation.?While very high rates, exceeding those required for in vivo photosynthesis, have been demonstrated for photoelectron transport to both natural and artificial electron acceptors and for photophosphorylation, rates of carbon reduction during photosynthesis by isolated chloroplasts have been disappointingly low. Fixation of C14-labeled carbon dioxide into intermediate compounds of the photosynthetic carbon reduction cycle by isolated chloroplasts was reported by Allen et al. in 1955.8 This ability to fix CO2 was diminished with broken chloroplasts but could be restored by various cofactors,9 and it was claimed that CO2 fixation by chloroplasts could be accomplished in the dark if the cofactors ATP and NADPH were added.'0 A report from the same laboratory (in 1960) listed fixation rates of 4.5-6 Mumoles C02/mg Chl/hr."1 Others12 found rates of about 4. It has long been assumed, and is verified by experiment in the present report, that spinach leaves should be capable of rates of CO2 fixation exceeding 200 Mmoles C02/mg Chl/hr. Fixation of CO2 has been stimulated by the addition of some intermediates of the carbon reduction cycle to broken chloroplasts,9 or whole chloroplasts.'3 Walker14 found a rate of 1.1 .tmoles C02/mg Chl/hr to be increased to 24.3 (and later,'5 36.9) upon the addition of ribose-5-phosphate to isolated chloroplasts prepared according to his method. With the addition of 71..5 ,moles of ribose-5-phosphate per mg chlorophyll to the reaction mixture, Walker's accelerated CO2 fixation rate resulted in a total fixation of 6.0 Amoles CO2 per mg chlorophyll during the 20-min period of incubation. Thus, the stimulated CO2 fixation rate could result from the operation of only part of the carbon reduction cycle utilizing ATP from the photochemical reactions to convert ribose-5-phosphate to ribulose-1,5-diphosphate, the carboxylation reaction substrate. It would appear that Walker's preparation had a ...

Regulation of glucose-6-phosphate dehydrogenase in spinach chloroplasts by ribulose 1,5-diphosphate and NADPH/NADP+ ratios

“…Young spinach leaves of approximately the same age were harvested from outdoor-grown plants after a 16-hour dark period.-Chloroplasts were -isolated according t9 the method of Jensen and Bassham [11]. The resulting chloroplast suspension in solution ~contained about 2mg chlorophyll/ml.…”

“…For the determination of NADP+ and NADPH levels in chloroplasts in the dark and during co 2 fixation in the light whole choloroplasts were 0 0 -7-isolated and were allo\'/ed to photosynthesize according to the procedure described by Jensen and Bassham [11]. A fluorometric method for measuring pyridine nucleotide levels in animal tissues described by Cartier [15] has been modified to detect very small amounts of pyridine nucleotides in chloroplasts while using a simple and rapid method for preparing samples from a kinetic experiment with ·photosynthesizing chloroplasts.…”

Synthesis of hamamelose‐diphosphate by isolated spinach chloroplasts

“…After filtration through cheesecloth the suspension was centrifuged for 1 min at 2000 g. The pellet was suspended in the buffer B of Jensen and Bassham [6] . Centrifugation at 30 g (1 min) removed whole cells and greater particles.…”

“…For CO2 -fixation the chloroplasts were incubated in 1 ml of the buffer C used by Jensen and Bassham [6]. The concentrations of NaH14C0s (4.6 mCi/ n-mole) and of chlorophyll were adjusted to those given by the same authors.…”

Fibrin membrane endowed with biological function

“…Chloroplasts were obtained from spinach as in [13]. Chloroplasts were suspended in 10 mM TricineNaOH (pH 7.8) and broken chloroplast suspension (1.5 mg chl/ml) was used in here.…”