Photosynthesis and biomass accumulation in young sugarcane plants grown under increasing ammonium supply in nutrient solution

Pissolato, Maria D.; Silveira, Neidiquele M.; Machado, Eduardo Caruso; Zambrosi, Fernando César Bachiega; Sodek, Ladaslav; Ribeiro, Rafael V.

doi:10.1007/s40626-019-00154-w

Cited by 9 publications

(11 citation statements)

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“…Our previous study revealed that sugarcane plants can be supplied with 30% NH 4 + in nutrient solution without compromising their photosynthesis and growth ( Pissolato et al, 2019a ). Thus, the NO 3 − :NH 4 + ratios 100:0 and 70:30 were chosen to represent the treatments with more and less NO 3 − , while supplying the same amount of nitrogen and avoiding NH 4 + toxicity.…”

Section: Methodsmentioning

confidence: 99%

“…Some crops have a preference for NH 4 + uptake ( Malagoli et al, 2000 ), but most studies have reported stress symptoms associated with NH 4 + toxicity ( Barreto et al, 2018 ; Boschiero et al, 2019 ). While Robinson et al (2011) reported the sugarcane preference for NH 4 + , Pissolato et al (2019a) found that increasing NH 4 + supply causes biomass reduction and photosynthesis impairment of sugarcane plants. Changing the N source, NO 3 − supply has been shown to increase the tolerance to abiotic stresses in maize ( Rios-Gonzales et al, 2002 ) and grass species ( Wang and Macko, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Nitric Oxide Synthesis Through Nitrate Supply Improves Drought Tolerance of Sugarcane Plants

et al. 2020

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Nitric oxide (NO) is an important signaling molecule associated with many biochemical and physiological processes in plants under stressful conditions. Nitrate reductase (NR) not only mediates the reduction of NO 3 − to NO 2 − but also reduces NO 2 − to NO, a relevant pathway for NO production in higher plants. Herein, we hypothesized that sugarcane plants supplied with more NO 3 − as a source of N would produce more NO under water deficit. Such NO would reduce oxidative damage and favor photosynthetic metabolism and growth under water limiting conditions. Sugarcane plants were grown in nutrient solution and received the same amount of nitrogen, with varying nitrate:ammonium ratios (100:0 and 70:30). Plants were then grown under well-watered or water deficit conditions. Under water deficit, plants exhibited higher root [NO 3 − ] and [NO 2 − ] when supplied with 100% NO 3 −. Accordingly, the same plants also showed higher root NR activity and root NO production. We also found higher photosynthetic rates and stomatal conductance in plants supplied with more NO 3 − , which was associated with increased root growth. ROS accumulation was reduced due to increases in the activity of catalase in leaves and superoxide dismutase and ascorbate peroxidase in roots of plants supplied with 100% NO 3 − and facing water deficit. Such positive responses to water deficit were offset when a NO scavenger was supplied to the plants, thus confirming that increases in leaf gas exchange and plant growth were induced by NO. Concluding, NO 3 − supply is an interesting strategy for alleviating the negative effects of water deficit on sugarcane plants, increasing drought tolerance through enhanced NO production. Our data also provide insights on how plant nutrition could improve crop tolerance against abiotic stresses, such as drought.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Nitric Oxide Synthesis Through Nitrate Supply Improves Drought Tolerance of Sugarcane Plants

et al. 2020

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“…Our previous study revealed that sugarcane plants can be supplied with 30% NH 4 + in nutrient solution without compromising their photosynthesis and growth (Pissolato et al , 2019). Thus, the NO 3 − :NH 4 + ratios 100:0 and 70:30 were chosen to represent the treatments with more and less NO 3 − , while supplying the same amount of nitrogen and avoiding NH 4 + toxicity.…”

Section: Methodsmentioning

confidence: 99%

“…Leaf CO 2 assimilation ( A n ), stomatal conductance ( g s ) and the effective quantum efficiency of photosystem II (□ PSII ) were measured under PPFD of 2000 μmol m −2 s −1 and air CO 2 concentration of 400 μmol mol −1 . The measurements were performed between 10:30 and 12:30 h, as carried out previously (Pissolato et al , 2019). The vapor pressure difference between leaf and air (VPDL) was 2.1±0.2 kPa and leaf temperature was 30±0.4°C during the evaluations.…”

Section: Methodsmentioning

confidence: 99%

“…Some crops have a preference for NH 4 + uptake (Malagoli et al , 2000), but most studies have reported stress symptoms associated with NH 4 + toxicity (Barreto et al , 2018; Boschiero et al , 2019). While Robinson et al (2011) reported the sugarcane preference for NH 4 + , Pissolato et al (2019) found that increasing NH 4 + supply causes biomass reduction and photosynthesis impairment of sugarcane plants. Changing the N source, NO 3 − supply has been shown to increase the tolerance to abiotic stresses in maize (Rios-Gonzalez et al , 2002; Zhang et al , 2012), wheat (Speer et al , 1994), pea (Frechilla et al , 2001), Populus simonii (Meng et al , 2016) and grass species (Wang and Macko, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced nitric oxide synthesis through nitrate supply improves drought tolerance of sugarcane plants

Pissolato

Silveira

Prataviera

et al. 2019

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37Nitric oxide (NO) is an important signaling molecule associated with many 38 biochemical and physiological processes in plants under stressful conditions. 39 Nitrate reductase (NR) not only mediates the reduction of NO 3¯ to NO 2¯ but also 40 reduces NO 2¯ to NO, a relevant pathway for NO production in higher plants. 41 Herein, we hypothesized that sugarcane plants supplied with more NO 3¯ as a 42 source of N would produce more NO under water deficit. Such NO would 43 reduce oxidative damage and favor photosynthetic metabolism and growth 44 under water limiting conditions. Sugarcane plants were grown in nutrient 45 solution and received the same amount of nitrogen, with varying 46 nitrate:ammonium ratios (100:0 and 70:30). Plants were then grown under well-47 watered or water deficit conditions, in which the osmotic potential of nutrient 48 solution was -0.15 and -0.75 MPa, respectively. Under water deficit, plants 49 exhibited higher root [NO 3¯] and [NO 2¯] when supplied with 100% NO 3¯. 50Accordingly, the same plants also showed higher root NR activity and root NO 51 production. We also found higher photosynthetic rates and stomatal 52 conductance in plants supplied with more NO 3¯, which improved root growth. 53 ROS accumulation was reduced due to increases in the activity of catalase in 54 leaves and superoxide dismutase and ascorbate peroxidase in roots of plants 55 supplied with 100% NO 3¯ and facing water deficit. Such positive responses to 56 water deficit were offset when a NO scavenger was supplied to the plants, thus 57 confirming that increases in leaf gas exchange and plant growth were induced 58 by NO. Concluding, NO 3¯ supply is an interesting strategy for alleviating the 59 negative effects of water deficit on sugarcane plants, increasing drought 60 tolerance through enhanced NO production. Our data also provide insights on 61 how plant nutrition could improve crop tolerance against abiotic stresses, such 62 as drought. 63 64 Akaike T, Maeda H. 1996. Quantitation of nitric oxide using 2-phenyl-4, 4, 5, 5tetramethylimidazoline-1-oxyl 3-oxide (PTIO). Methods in Enzymology 268, 211-221. Albertos P, Romero-Puertas MC, Tatematsu K, Mateos I, Sánchez-Vicente I, Nambara E, Lorenzo O. 2015. S-nitrosylation triggers ABI5 degradation to promote seed germination and seedling growth. Nature Communications 6, 8669. Alderton WK, Cooper CE, Knowles RG. 2001. Nitric Oxide Synthases: structure, function and inhibition. Biochemistry Journal 357, 593-615. Alexieva V, Sergiev I, Mapelli S, Karanov E. 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell and Environment 24, 1337-1344. Andrés RM, Peralta AS, Vázquez JPS, Mendivil SN, Cabrera JAP, Torres MES, Dubrovsky JG, Ruan VL. 2015. The nitric oxide production in the moss Physcomitrella patens is mediated by nitrate reductase. PloS One 10, e0119400. Arasimowicz-Jelonek M, Floryszak-Wieczorek J, Kubiś J. 2009. Involvement of nitric oxide in water stress-induced responses of cucumber roots. Plant...

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Mitigating water stress by increasing NO3−: NH4+ ratio in young Eucalyptus urophylla plants

Soares

Cairo

Silva

et al. 2021

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Photosynthesis and biomass accumulation in young sugarcane plants grown under increasing ammonium supply in nutrient solution

Abstract: The aim of this study was to evaluate the sugarcane responses to varying ammonium:nitrate (NH 4 ? :NO 3-) ratio and to reveal how much NH 4 ?

Cited by 9 publications

References 64 publications

Enhanced Nitric Oxide Synthesis Through Nitrate Supply Improves Drought Tolerance of Sugarcane Plants

Enhanced Nitric Oxide Synthesis Through Nitrate Supply Improves Drought Tolerance of Sugarcane Plants

Enhanced nitric oxide synthesis through nitrate supply improves drought tolerance of sugarcane plants

Mitigating water stress by increasing NO3−: NH4+ ratio in young Eucalyptus urophylla plants

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