Photostability of dye molecules trapped in solid matrices

Abstract

The photostability of dye molecules trapped in transparent solid matrices synthesized by the solgel technique was studied both experimentally and theoretically using a model with numerical and approximate analytical solutions. The model is based on a one-photon photodestruction process with the creation of an absorbing bleached molecule. We give the number of photons that different trapped dye molecules can absorb on average before they are bleached. Dyes such as Perylene Red, Perylene Orange, Pyrromethenes 567 and 597, Rhodamines 6G and B, DCM, a Xanthylium salt, and Neon Red were investigated; significant differences were observed. Some dye molecules in solvents were also studied; increased stability resulted when the molecules were trapped in solid matrices.

Toward millions of laser pulses with pyrromethene- and perylene-doped xerogels

“…We generally used pump energy levels of 1 mJ, and the repetition rate was varied to compare the relative importance of the two main dye degradation processes, photodegradation and thermodegradation, as analyzed in Ref. 8.…”

“…However special attention must be paid to the perylenes that appear to possess, if thermodegradation can be minimized, a higher potential effect on lifetimes in terms of photostability. 8 However in the case of these perylene dyes, we still have to enhance the energy conversion efficiencies and increase the matrix thermal conductivity.…”

Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level

“…ingle molecule imaging has enabled the exploration of biomolecular dynamics and has revealed processes at work that are lost by extrapolation of ensemble assays (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). During the past few decades, optical single-molecule techniques such as single-fluorophore tracking (4,5) and single-molecule fluorescence resonance energy transfer (1,(6)(7)(8) have proven to be effective tools for such purposes and have provided new biological information related to enzyme activity (6); transcription (7); protein dynamics (8); identification of rare intermediates and kinetic heterogeneity during RNA folding (5,9); and RNAprotein interactions (10).…”

“…Despite recent advances, however, most studies rely on biomolecules either immobilized on substrates or confined in a matrix, and observation of singlemolecule behaviors inside live cells remains challenging (11)(12)(13)(14)(15). The inherent limitations of molecular dyes for single-molecule imaging, including low signal intensities, complex blinking phenomena, and photobleaching, are even more pronounced in live cell studies (16). Specifically, the fast photobleaching (less than a minute) of fluorescent dyes (17) hinders the ability to continuously monitor signaling pathways that transpire on the time scale of hours.…”

Theoretical and experimental study of the bleaching of a dye in a film-polymerization process

“…[1][2][3][4] The dye's quantum yield and the molar-absorption coefficient are two basic parameters that will determine the photochemical behavior of the material into which the dye has been introduced. The quantum yield of a photochemical reaction is the number of product moles that form divided by the number of photons absorbed.…”