Photostability and thermal stability of indocyanine green

Hölzer, W.; Mauerer, M.; Penzkofer, Alfons; Szeimies, R.-M.; Abels, Christoph; Landthaler, Míchael; Bäumler, Wolfgang

doi:10.1016/s1011-1344(98)00216-4

Cited by 183 publications

(162 citation statements)

References 30 publications

Supporting

Mentioning

150

Contrasting

Unclassified

Order By: Relevance

“…During this study, we also found the initial probability of photobleaching for 10 M ICG concentration in water to beϳ1 ϫ 10 −5 , which is approximately two orders of magnitude smaller than that reported in Ref. 28.…”

Section: Photobleaching Dynamics Of Icgcontrasting

confidence: 47%

“…Most notable among previous works on the photodegradation process in ICG is the work of Holzer et al 28 The study, however, is based on either very low ͑e.g., 10 M͒ or very high ͑e.g., 1.5 mM-toxic level 29 ͒ concentrations of ICG. Moreover, the work was predominantly geared toward characterizing the stability of above two concentrations of ICG ͑in different solvents͒ based on the initial probability of photobleaching.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent

Yaqoob

McDowell

et al. 2006

J. Biomed. Opt.

View full text Add to dashboard Cite

Abstract. The use of indocyanine green ͑ICG͒, a U.S. Food and Drug Administration approved dye, in a pump-probe scheme for molecular contrast optical coherence tomography ͑MCOCT͒ is proposed and demonstrated for the first time. In the proposed pump-probe scheme, an optical coherence tomography ͑OCT͒ scan of the sample containing ICG is first acquired. High fluence illumination ͑ϳ190 kJ/ cm 2 ͒ is then used to permanently photobleach the ICG molecules-resulting in a permanent alteration of the overall absorption of the ICG. A second OCT scan is next acquired. The difference of the two OCT scans is used to determine the depth resolved distribution of ICG within a sample. To characterize the extent of photobleaching in different ICG solutions, we determine the cumulative probability of photobleaching, B,cum , defined as the ratio of the total photobleached ICG molecules to the total photons absorbed by the ground state molecules. An empirical study of ICG photobleaching dynamics shows that B,cum decreases with fluence as well as with increasing dye concentration. The quantity B,cum is useful for estimating the extent of photobleaching in an ICG sample ͑MCOCT contrast͒ for a given fluence of the pump illumination. The paper also demonstrates ICG-based MCOCT imaging in tissue phantoms as well as within stage 54 Xenopus laevis.

show abstract

Section: Photobleaching Dynamics Of Icgcontrasting

confidence: 47%

Section: Introductionmentioning

confidence: 99%

Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent

Yaqoob

McDowell

et al. 2006

J. Biomed. Opt.

View full text Add to dashboard Cite

show abstract

“…Similarly, the peak absorbance of 6.4 mM ICG in fresh pig and human plasma was ≈ 10% higher than that of ICG in water and direct light [35]. In addition to the effects of albumin on ICG absorption, other investigators have reported that the natural degradation kinetics of ICG at room temperature is reduced in the presence of HSA [36].…”

mentioning

confidence: 92%

“…Although changes in optical properties of ICG have been observed in the presence of albumin, previous studies have been limited to either a fixed HSA level [28], [37], or have not investigated the fluorescence characteristics of ICG over a broad range of ICG concentration in presence of serum proteins [35], [36]. To fill this void, we present a systematic study of the effects of serum at physiological levels and temperature on the absorption and fluorescence characteristics of ICG in the range of 0.6-25.8 μM.…”

mentioning

confidence: 99%

Revisiting Indocyanine Green: Effects of Serum and Physiological Temperature on Absorption and Fluorescence Characteristics

Jung

Vullev

Anvari

2014

IEEE J. Select. Topics Quantum Electron.

View full text Add to dashboard Cite

Abstract-Indocyanine green (ICG) remains as the only near infrared dye approved by the FDA. Despite its long history of usage in clinical medicine, a systematic study of the effects of serum proteins at physiologically relevant levels and temperature on absorption and fluorescence characteristics of ICG has been missing. We incubated ICG at concentrations in the range of 0.6-25.8 μM in McCoy's 5a cell culture medium, without and with supplemental fetal bovine serum (FBS) at 5% and 10% levels. Our analyses of absorption and fluorescence spectra indicate that the peak absorbance of ICG associated with its monomeric form increases in the presence of FBS. For example, at ICG concentration of 25.8 μM, the monomer absorbance is increased by nearly 100% in the presence of 10% FBS. Similarly, there is an increase in the relative fluorescence quantum yield of ICG, by as much as nearly 3.5 times in the presence of FBS. When incubated at 37• C, the presence of FBS in the cell culture medium helps maintain the monomeric absorption of ICG and sustain the increased fluorescence emission. We offer explanations to describe the possible photophysical mechanisms underlying the observed effects and discuss the importance of these results to in-vivo applications of ICG.

show abstract

“…Besides this, the stability of fluorochromes both in vitro and in vivo is critical, as opsonization and rapid clearance can greatly limit their application for in vivo imaging 11 . Other effects such as poor stability and low sensitivity or cytotoxic effects on target organs as seen with indocyanine green (ICG) [12][13][14][15][16] , are unwanted and must be taken into consideration when designing probes for in vivo imaging. These observations have led to the active development of several preclinical NIR fluorochromes, nanoparticles as well as new techniques for the in vivo imaging of inflammatory processes, cancer and for image-guided surgery 2.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for <em>In Vivo</em> Optical Imaging

Tansi

Rüger

Rabenhold

et al. 2015

JoVE

View full text Add to dashboard Cite

Optical imaging offers a wide range of diagnostic modalities and has attracted a lot of interest as a tool for biomedical imaging. Despite the enormous number of imaging techniques currently available and the progress in instrumentation, there is still a need for highly sensitive probes that are suitable for in vivo imaging. One typical problem of available preclinical fluorescent probes is their rapid clearance in vivo, which reduces their imaging sensitivity. To circumvent rapid clearance, increase number of dye molecules at the target site, and thereby reduce background autofluorescence, encapsulation of the near-infrared fluorescent dye, DY-676-COOH in liposomes and verification of its potential for in vivo imaging of inflammation was done. DY-676 is known for its ability to self-quench at high concentrations. We first determined the concentration suitable for self-quenching, and then encapsulated this quenching concentration into the aqueous interior of PEGylated liposomes. To substantiate the quenching and activation potential of the liposomes we use a harsh freezing method which leads to damage of liposomal membranes without affecting the encapsulated dye. The liposomes characterized by a high level of fluorescence quenching were termed Lip-Q. We show by experiments with different cell lines that uptake of Lip-Q is predominantly by phagocytosis which in turn enabled the characterization of its potential as a tool for in vivo imaging of inflammation in mice models. Furthermore, we use a zymosan-induced edema model in mice to substantiate the potential of Lip-Q in optical imaging of inflammation in vivo. Considering possible uptake due to inflammation-induced enhanced permeability and retention (EPR) effect, an always-on liposome formulation with low, non-quenched concentration of DY-676-COOH (termed LipdQ) and the free DY-676-COOH were compared with Lip-Q in animal trials. Video LinkThe video component of this article can be found at

show abstract

Photostability and thermal stability of indocyanine green

Cited by 183 publications

References 30 publications

Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent

Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent

Revisiting Indocyanine Green: Effects of Serum and Physiological Temperature on Absorption and Fluorescence Characteristics

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for <em>In Vivo</em> Optical Imaging

Contact Info

Product

Resources

About