Photosensitizer Effects of Artificial Red Cells on Dye Laser Irradiation in an Animal Model Assuming Port-Wine Stain Treatment

Rikihisa, Naoaki; Watanabe, Shôji; Satoh, Kaneshige; Saito, Yoshiaki; Sakai, Hiromi

doi:10.1097/prs.0000000000003082

Cited by 16 publications

(24 citation statements)

References 18 publications

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“…Rikihisa et al reported utilization of HbV as a photosensitizer, a target of laser treatment of port-wine stain (capillary malformation) [44], because injection of HbV can increase capillary Hb levels effectively as depicted in Figure 3B, producing more heat and photocoagulation.…”

Section: Potential Usage Of Hbv As An Oxygen Carrier For Ischemic mentioning

confidence: 99%

Overview of Potential Clinical Applications of Hemoglobin Vesicles (HbV) as Artificial Red Cells, Evidenced by Preclinical Studies of the Academic Research Consortium

Sakai

2017

JFB

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Hemoglobin (Hb) is the most abundant protein in whole blood. This fact implies that the oxygen binding and releasing function of Hb is the most vital for sustaining life. All Hb is compartmentalized in red blood cells (RBCs) with corpuscular Hb concentration of about 35 g/dL, covered with a thin biomembrane. In spite of its abundance, Hb sometimes shows toxicity once it is leaked from RBCs. The shielding effect of the RBC membrane is physiologically important. Based on this structural importance, we have studied artificial red cells (Hb vesicles, HbV) as artificial oxygen carriers, which encapsulate a purified and concentrated Hb solution in phospholipid vesicles, mimicking the cellular structure of RBCs. Our academic research consortium has clarified the safety and efficacy of this HbV, aiming at clinical applications. Because of some superior characteristics to those of RBCs, HbV has the potential for use not only as a transfusion alternative but also for oxygen and carbon monoxide therapeutics, perfusate for transplant organs, and photosensitizer. In this review paper, such potential applications are summarized.

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Section: Potential Usage Of Hbv As An Oxygen Carrier For Ischemic mentioning

confidence: 99%

Overview of Potential Clinical Applications of Hemoglobin Vesicles (HbV) as Artificial Red Cells, Evidenced by Preclinical Studies of the Academic Research Consortium

Sakai

2017

JFB

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“…Following the injection of Hb-Vs (at a dose of one-tenth of the circulating blood volume), the microvessels were observed to expand and to become markedly dense. The specific area of the microvessels in a bird’s-eye view increased by 20% after the injection (Figure 4) [1]. …”

Section: Break Through the Limits Of Laser Treatment Against Pws Wmentioning

confidence: 99%

“…Absorbance at 595 nm at various concentrations of Hb was determined using a spectrophotometer. The hBlood, Hb-V and Mixed group samples all showed the same absorbance at 595 nm at all concentrations of Hb (Figure 6) [1]. …”

Section: Break Through the Limits Of Laser Treatment Against Pws Wmentioning

confidence: 99%

“…We used a securing device to maintain a fixed distance between the samples and the laser handset. The three sample groups showed the same change in temperature after irradiation with the dye laser, with Hb concentration maintained at 10 g/dL [1]. …”

Section: Break Through the Limits Of Laser Treatment Against Pws Wmentioning

confidence: 99%

“…We investigated the use of Hb-Vs as photosensitizers in the dye laser treatment of port-wine stains (PWSs). We summarize our results of in vivo and in vitro experiments to measure the aptitude of Hb-V as a photosensitizer, and discuss the advantages of this method [1,2,3]. …”

Section: Introductionmentioning

confidence: 99%

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Artificial Red Blood Cells as Potential Photosensitizers in Dye Laser Treatment Against Port-Wine Stains

Rikihisa

Watanabe

Saito

et al. 2017

JFB

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We suggest a novel method that uses artificial blood cells (hemoglobin vesicles, Hb-Vs) as photosensitizers in dye laser treatment (at 595-nm wavelength) for port-wine stains (i.e., capillary malformations presenting as red birthmarks) based on the results of animal experiments. As compared with human red blood cells, Hb-Vs have the same absorbance of 595 nm wavelength light and produce the same level of heat following dye laser irradiation. Small sized Hb-Vs (250 nm) distribute in the plasma phase in blood and tend to flow in the marginal zone of microvessels. Intravenous injections of Hb-Vs caused the dilatation of microvessels, and dye laser treatment with Hb-Vs destroyed the vessel wall effectively. Following the intravenous injection of Hb-Vs, the microvessels contained more Hb that absorbed laser photons and produced heat. This extra Hb tended to flow near the endothelial cells, which were the target of the laser treatment. These attributes of Hb-Vs will potentially contribute to enhancing the efficacy of dye laser treatment for port-wine stains. Hemoglobin is a type of porphyrin. Thus, our proposed treatment may have aspects of photodynamic therapy using porphyrin that leads to a cytotoxicity effect by active oxygen.

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Gold nanospheres enhanced photothermal therapy in a rat model

Liu

Chen

et al. 2018

Lasers Surg Med

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Photosensitizer Effects of Artificial Red Cells on Dye Laser Irradiation in an Animal Model Assuming Port-Wine Stain Treatment

Cited by 16 publications

References 18 publications

Overview of Potential Clinical Applications of Hemoglobin Vesicles (HbV) as Artificial Red Cells, Evidenced by Preclinical Studies of the Academic Research Consortium

Overview of Potential Clinical Applications of Hemoglobin Vesicles (HbV) as Artificial Red Cells, Evidenced by Preclinical Studies of the Academic Research Consortium

Artificial Red Blood Cells as Potential Photosensitizers in Dye Laser Treatment Against Port-Wine Stains

Gold nanospheres enhanced photothermal therapy in a rat model

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