Photosensitized Inactivation of <i>Plasmodium falciparum‐</i> and <i>Babesia divergens</i>‐Infected Erythrocytes in Whole Blood by Lipophilic Pheophorbide Derivatives

Grellier, Philippe; Santús, René; Mouray, Elisabeth; Agmon, Vered; Mazière, Jean‐Claude; Rigomier, Daniel; Dagan, Arie; Gátt, Shimon; Schrével, Joseph

doi:10.1046/j.1423-0410.1997.7240211.x

Cited by 47 publications

(36 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4C and D), we were unable to find the "ideal" experimental conditions which specifically lysed intracellular amastigotes but which left their host cells substantially intact or viable. This is different from the observations of blood parasites, i.e., Trypanosoma cruzi (15), Plasmodium (19,30), and Babesia (19), whose susceptibilities to photodynamic treatment have been exploited for their elimination from blood bank samples. It was also noted previously (29) that photosensitized Leishmania caused the cytolysis of infected host cells.…”

Section: Discussioncontrasting

confidence: 72%

Photodynamic Sensitization of Leishmania amazonensis in both Extracellular and Intracellular Stages with Aluminum Phthalocyanine Chloride for Photolysis In Vitro

Dutta

Ray

Kolli

et al. 2005

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Leishmania amazonensis, a causative agent of cutaneous leishmaniasis, is susceptible in vitro to lightmediated cytolysis in the presence of or after pretreatment with the photosensitizer aluminum phthalocyanine chloride. Cytolysis of both promastigotes and axenic amastigotes required less photosensitizer (e.g., one g · ml ؊1 ) and a lower light dose (e.g., 1.5 J · cm ؊2 ) than did the mammalian cells examined for comparison. Exposure of Leishmania cells to the photosensitizer alone had little effect on their viability, as judged from their motility, growth, and/or retention of green fluorescent proteins genetically engineered for episomal expression. Fluorimetric assays for cell-associated and released green fluorescence proteins proved to be even more sensitive for the evaluation of cell viability than microscopy for the evaluation of motility and/or integrity. Axenic amastigotes pretreated with the photosensitizer infected macrophages of the J774 line but were lysed intracellularly when the infected cells were exposed to light. Addition of the photosensitizer to the already infected cells produced no effect on their intracellular parasites. However, light irradiation lysed these macrophages and also those infected with parasites preincubated with the photosensitizer at a concentration of 5 g · ml ؊1 or higher. Photosensitized Leishmania cells are highly susceptible to cytolysis, apparently due to the generation of reactive oxidative species on light illumination, suggestive of inefficiency of their antioxidant mechanisms. Efficient delivery of photosensitizers to intracellular Leishmania is expected to increase their therapeutic potentials against leishmaniasis.

show abstract

Section: Discussioncontrasting

confidence: 72%

Photodynamic Sensitization of Leishmania amazonensis in both Extracellular and Intracellular Stages with Aluminum Phthalocyanine Chloride for Photolysis In Vitro

Dutta

Ray

Kolli

et al. 2005

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…However, merocyanine 540 appeared to have little specificity to infected RBCs, as the singlet oxygen produced by this compound was subsequently shown to damage uninfected erythrocytes as well [44][45][46][47]. Further development of pheophorbide derivatives [48] and silicon phthalocyanines [49,50], with respect to the photoinactivation of P. falciparum, have not appeared in the literature.…”

Section: Discussionmentioning

confidence: 99%

Inactivation ofPlasmodium falciparumby Photodynamic Excitation of Heme‐Cycle Intermediates Derived from δ‐Aminolevulinic Acid

Smith

Kain

2004

J INFECT DIS

View full text Add to dashboard Cite

Transfusion-transmitted malaria (TTM), especially that caused by Plasmodium falciparum, is of great concern in malaria-endemic areas. As a result of increased international travel, migration, and the spread of drugresistant parasites, TTM is also a growing problem in industrialized nations. An effective and inexpensive means of inactivating malaria parasites in blood products would represent an important advance. In this report, we demonstrate that photoactivation of plasmodial heme-cycle intermediates, derived from supplemental d-aminolevulinic acid (ALA), by exposure to simple white light in the presence of ALA, reduces P. falciparum in culture to levels that are undetectable by light microscopy or lactate dehydrogenase assay. Photodynamic excitation of presumed heme-cycle intermediates, which was revealed by fluorescence microscopy, did not appear to adversely affect the viability of erythrocytes. These data suggest that this pathogeninactivation strategy, which uses inexpensive reagents and white light, may represent an appropriate means of inactivating malaria parasites in blood products in resource-poor settings.

show abstract

“…[5][6][7][8][9][10] Materials that are inherently antimicrobial could prevent the transmission of pathogenic microorganisms autonomously, without the potential for human error leading to infections. These materials have toxic, and are therefore not expected to have an adverse effect on human health; ii) their antimicrobial action is primarily mediated by singlet oxygen, 17,18 which has several benefits including a short lifetime before it decays back to environmentally benign ground state (triplet) oxygen, 19 the ability to inactivate bacteria that are not in direct contact with the material, 20,21 as well as potentially broad antimicrobial efficacy against bacteria, [22][23][24][25] yeast, [26][27][28][29] viruses, [30][31][32][33] and parasites 34,35 ; and iii) as singlet oxygen causes nonspecific damage, it is thought that microbes will be unable to develop resistance to this mode of action. 24,36,37 Early investigations into aPDI materials were initially curtailed by the belief that, to be effective, photosensitizers needed to either be embedded in the cellular envelope or internalized into the bacteria.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Antimicrobial Efficacy of Photomicrobicidal Cellulose Paper

Carpenter¹,

Scholle²,

Sadeghifar

et al. 2015

Biomacromolecules

View full text Add to dashboard Cite

Toward our goal of scalable, antimicrobial materials based on photodynamic inactivation, paper sheets comprised of photosensitizer-conjugated cellulose fibers were prepared using porphyrin and BODIPY photosensitizers, and characterized by spectroscopic (infrared, UV-vis diffuse reflectance, inductively coupled plasma optical emission) and physical (gel permeation chromatography, elemental, and thermal gravimetric analyses) methods. Antibacterial efficacy was evaluated against Staphylococcus aureus (ATCC-2913), vancomycin-resistant Enterococcus faecium (ATCC-2320), Acinetobacter baumannii (ATCC-19606), Pseudomonas aeruginosa (ATCC-9027), and Klebsiella pneumoniae (ATCC-2146). Our best results were achieved with a cationic porphyrin-paper conjugate, Por((+))-paper, with inactivation upon illumination (30 min, 65 ± 5 mW/cm(2), 400-700 nm) of all bacterial strains studied by 99.99+% (4 log units), regardless of taxonomic classification. Por((+))-paper also inactivated dengue-1 virus (>99.995%), influenza A (∼ 99.5%), and human adenovirus-5 (∼ 99%). These results demonstrate the potential of cellulose materials to serve as scalable scaffolds for anti-infective or self-sterilizing materials against both bacteria and viruses when employing a photodynamic inactivation mode of action.

show abstract

Photosensitized Inactivation of Plasmodium falciparum‐ and Babesia divergens‐Infected Erythrocytes in Whole Blood by Lipophilic Pheophorbide Derivatives

Cited by 47 publications

References 23 publications

Photodynamic Sensitization of Leishmania amazonensis in both Extracellular and Intracellular Stages with Aluminum Phthalocyanine Chloride for Photolysis In Vitro

Photodynamic Sensitization of Leishmania amazonensis in both Extracellular and Intracellular Stages with Aluminum Phthalocyanine Chloride for Photolysis In Vitro

Inactivation ofPlasmodium falciparumby Photodynamic Excitation of Heme‐Cycle Intermediates Derived from δ‐Aminolevulinic Acid

Synthesis, Characterization, and Antimicrobial Efficacy of Photomicrobicidal Cellulose Paper

Contact Info

Product

Resources

About