Photorhabdus viridis sp. nov. Isolated from Heterorhabditis zealandica Entomopathogenic Nematodes

Abstract

A novel bacterial species, Photorhabdus viridis sp. nov., represented by strain GreenT, isolated from Heterorhabditis zealandica MJ2C entomopathogenic nematodes, is described. Phylogenetic reconstructions using 16S rRNA gene sequences show that strain GreenT is closely related to P. thracensis DSM 15199 T. The 16rRNA gene sequences of these two strains are 98.8% identical. Phylogenetic reconstructions using whole-genome sequences show that strain GreenT is closely related to P. tasmaniensis DSM 22387 T, P. thracensis DSM 15199 T, and P. temperata DSM 14550 T. Digital DNA-DNA hybridization (dDDH) values between strain GreenT and its three more close relative species, P. tasmaniensis DSM 22387 T, P. thracensis DSM 15199 T, and P. temperata DSM 14550 T, are 49%, 59%, and 59%, respectively. In addition, average nucleotide identity (ANI) values between GreenT and P. tasmaniensis DSM 22387 T, P. thracensis DSM 15199 T, and P. temperata DSM 14550 T are 92.4%, 94.4%, and 94.6%, respectively. The novel species also differs in their biochemical capacities from the biochemical capacities of their more closely related taxa. The following biochemical tests may be particularly useful in this context: Arginine dihydrolase, gelatinase, and glucose and mannitol oxidation. Given the clear phylogenetic separation, the sequence divergence values, and the phenotypic differences, we conclude that strain GreenT represents a novel bacterial species, for which we propose the name Photorhabdus viridis sp. nov. with GreenT (= CCM 9407 T = CCOS 2117 T = MJ2CT) as the type strain. Our study contributes to a better understanding of the taxonomy and biodiversity of an important bacterial group with great biotechnological and agricultural potential.