Photorhabdus luminescens subsp. akhurstii SL0708 pathogenicity in Spodoptera frugiperda (Lepidoptera: Noctuidae) and Galleria mellonella (Lepidoptera: Pyralidae)

Salazar-Gutiérrez, Julián David; Castelblanco, Andrés; Rodriguez-Bocanegra, María Ximena; Terán, Wilson; Sáenz-Aponte, A.

doi:10.1016/j.aspen.2017.08.001

Cited by 14 publications

(10 citation statements)

References 53 publications

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“…The crude extract from the strain ETL had higher fungal MIC properties (≤1 mg/mL of MIC) against A. flavus and A. niger than a crude extract from another reported EPNB (4 mg/mL ant 5 mg/mL for A. flavus and A. niger, respectively) [35], although a pur-antifungal compound (cyclo(l-Prod-Leu)) isolated from it showed greater MIC values of 0.008 mg/mL and 0.032 mg/mL against A. flavus and A. niger, respectively [35]. Members of the Photorhabdus produce numerous SMs with various antimicrobial activities [5,12] to suit the entomopathogenic lifestyle with their nematode vectors and to contribute to the symbiotic association with the nematode host by providing a monoxenic growth environment while killing off competitive microbes and insect larvae, and providing nutrients for both the nematodes and bacteria [4,[36][37][38][39]. This could justify the notable inhibitory activity found in this study.…”

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Photorhabdus sp. ETL Antimicrobial Properties and Characterization of Its Secondary Metabolites by Gas Chromatography–Mass Spectrometry

Lulamba

Green

Serepa-Dlamini

2021

Life

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Entomopathogenic nematodes (EPNs) are known to be highly pathogenic to insect pests, due to their associated symbiotic bacteria, which produce virulence factors, exo-enzymes and other harmful secondary metabolites to conquer, kill, and degrade their insect hosts. However, these properties are not fully characterized. This study reports on the antimicrobial activities of Photorhabdus sp. strain ETL, symbiotically associated to an insect pathogenic nematode, Heterorhabditis zealandica, against human pathogenic bacteria and toxigenic fungi, as well as the non-targeted profiling of its secondary metabolites (SMs) using gas chromatography coupled to high-resolution time-of-flight mass spectrometry. Fatty acids including 3-eicosene, (E)-; 5-eicosene, (E)-; eicosene; 9-octadecenamide; undecanoic acid with shown antimicrobial activities were detected. This provided more insight on the composition and bioactivities of SMs produced by the Photorhabdus sp.

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Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Photorhabdus sp. ETL Antimicrobial Properties and Characterization of Its Secondary Metabolites by Gas Chromatography–Mass Spectrometry

Lulamba

Green

Serepa-Dlamini

2021

Life

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“…Bacterium metabolic phase I is required for EPN production, particularly due to four metabolic characteristics: (1) capacity to degrade media into compounds that can be assimilated and necessary for IJs, distinctively sterols by lytic enzymes; (2) secretion of "feeding signals" to induce development and production of new IJs generations; (3) capacity to establish symbiosis or re-associate/adherence to the nematode's intestines; and (4) secretion of antibiotic substances, avoiding contamination in the media (Ehlers 2001;Salazar-Gutiérrez et al 2017). Consequently, it is most important to maintain phase I during in vitro EPNs production to increase recovery percentage and to obtain high IJ yield during the production process (Stock et al 2017).…”

Section: Resultsmentioning

confidence: 99%

“…After approximately 24 h, IJs which are suspended in an enriched nutrient broth are inoculated into the bacterial culture. To check the entompathogenicity, the greater wax moth, Galleria mellonella Linnaeus (Lepidoptera: Pyralidae), was infected by the nematodes along with its symbiont, and its death occurs within 48 h, and the insect pathogenicity of the H. indica-P. luminescens combination is confirmed (Salazar-Gutiérrez et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Growth kinetics and pathogenicity of Photorhabdus luminescens subsp. akhurstii SL0708

Orozco-Hidalgo

Quevedo-Hidalgo

Sáenz-Aponte

2019

Egypt J Biol Pest Control

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Photorhabdus luminescens subsp. akhurstii SL0708 (Enterobacteriaceae) is a symbiont of the entomopathogenic nematode (EPN), Heterorhabditis indica SL0708 (Nematoda: Rhabditida), used for insect pest biological control. In the present study, P. luminescens subsp. akhurstii SL0708 growth kinetic was evaluated considering growth and metabolic phases (phase I, intermediate phase, phase II), as well as pathogenicity. The study can be useful in determining bacterium feeding times in H. indica SL0708 production in liquid culture media. The logarithmic phase of the growth of bacterium was from 0 to 24 h, with a specific growth velocity of 0.21 h −1 ; during this phase, bacterium at metabolic phase I was detected. Maximum bioluminescence was registered at 24 h (3.437 luminescence AU). Finally, it was evidenced that the bacterial metabolic phase had an effect on the greater wax moth, Galleria mellonella L., larvae mortality rate. Moreover, biochemical tests were the same for all P. luminescens subsp. akhurstii SL0708 sampling times. This research is particularly relevant, since no reports are available on this bacterium isolate in Colombia. In the future, this will allow massive H. indica SL0708 production, because when preincubated with its symbiont, it provides essential nutrients for the EPNs development and reproduction.

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“…The LC 50 dose of the strain K-1 was 1010 cells per S. litura sixth-instar larvae in 48 h [ 53 ]. A 100% mortality of both the fall armyworms, Spodoptera frugiperda and G. mellonella , was also attained after 48 h of treatment with the strain P. luminescens akhurstii SL0708 at 1 × 10 3 –1 × 10 4 CFU/larva [ 66 ]. On the other hand, P. temperata showed oral toxicity to the olive moth, Prays oleae , with an LC 50 of 58.1 × 10 6 cells/mL [ 67 ].…”

Section: Pathogenicity Of Photorhabdus Sppmentioning

confidence: 99%

“…For instance, a cumulative percent mortality of 63% and 100% for G. mellonella, but 10% and 93% for S. frugiperda, was achieved after 72 h of injecting intra- or extra-cellular extracts of the strain P. luminescens akhurstii SL0708, respectively. These differences also indicate the impact of extracellular factors in pathogenicity [ 66 ]. Those authors detected proteases, esterases, ureases, hemolysins and siderophores as responsible for the high pathogenicity/extra-cellular activities.…”

Section: Pathogenicity Of Photorhabdus Sppmentioning

confidence: 99%

Photorhabdus spp.: An Overview of the Beneficial Aspects of Mutualistic Bacteria of Insecticidal Nematodes

Abd-Elgawad

2021

Plants

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The current approaches to sustainable agricultural development aspire to use safer means to control pests and pathogens. Photorhabdus bacteria that are insecticidal symbionts of entomopathogenic nematodes in the genus Heterorhabditis can provide such a service with a treasure trove of insecticidal compounds and an ability to cope with the insect immune system. This review highlights the need of Photorhabdus-derived insecticidal, fungicidal, pharmaceutical, parasiticidal, antimicrobial, and toxic materials to fit into current, or emerging, holistic strategies, mainly for managing plant pests and pathogens. The widespread use of these bacteria, however, has been slow, due to cost, natural presence within the uneven distribution of their nematode partners, and problems with trait stability during in vitro culture. Yet, progress has been made, showing an ability to overcome these obstacles via offering affordable mass production and mastered genome sequencing, while detecting more of their beneficial bacterial species/strains. Their high pathogenicity to a wide range of arthropods, efficiency against diseases, and versatility, suggest future promising industrial products. The many useful properties of these bacteria can facilitate their integration with other pest/disease management tactics for crop protection.

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Photorhabdus luminescens subsp. akhurstii SL0708 pathogenicity in Spodoptera frugiperda (Lepidoptera: Noctuidae) and Galleria mellonella (Lepidoptera: Pyralidae)

Cited by 14 publications

References 53 publications

Photorhabdus sp. ETL Antimicrobial Properties and Characterization of Its Secondary Metabolites by Gas Chromatography–Mass Spectrometry

Photorhabdus sp. ETL Antimicrobial Properties and Characterization of Its Secondary Metabolites by Gas Chromatography–Mass Spectrometry

Growth kinetics and pathogenicity of Photorhabdus luminescens subsp. akhurstii SL0708

Photorhabdus spp.: An Overview of the Beneficial Aspects of Mutualistic Bacteria of Insecticidal Nematodes

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