Photoresponsive colorimetric immunoassay based on chitosan modified AgI/TiO2 heterojunction for highly sensitive chloramphenicol detection

Chang, Honghong; Lv, Jia; Zhang, Haochun; Zhang, Bing; Wei, Wenlong; Qiao, Yan

doi:10.1016/j.bios.2016.09.002

Cited by 70 publications

(26 citation statements)

References 37 publications

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“…Over the past decade, magnetic materials have attracted considerable attention due to their excellent performance in the fields of bioassays, − drug delivery, − electronics, , magnetic resonance imaging, − and environmental remediation. − As typical magnetic materials, magnetic beads (MBs) can be concentrated, trapped, or used in rapid separation by applying a magnetic field. Owing to their outstanding properties, such as high loading capacity, high magnetic susceptibility, and good biocompatibility, MBs have extensively been used for label-based bioassays such as immunoassays − and DNA assays. − Particularly, label-based chemiluminescence (CL) technology with MBs has been successfully commercialized in immunoassays. However, they still suffer from complicated labeling, purification, and washing procedures and need secondary antibody, which makes them labor-intensive, time-consuming, and expensive.…”

mentioning

confidence: 99%

Highly Chemiluminescent Magnetic Beads for Label-Free Sensing of 2,4,6-Trinitrotoluene

Kong

Zhao²,

Zhu

et al. 2017

Anal. Chem.

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Until now, despite the great success acquired in scientific research and commercial applications, magnetic beads (MBs) have been used for nothing more than a carrier in most cases in bioassays. In this work, highly chemiluminescent magnetic beads containing N-(4-aminobutyl)-N-ethyl isoluminol (ABEI) and Co (Co/ABEI/MBs) were first synthesized via a facile strategy. ABEI and Co were grafted onto the surface of carboxylated MBs by virtue of a carboxyl group and electrostatic interaction. The as-prepared Co/ABEI/MBs exhibited good paramagnetic properties, satisfactory stability, and intense chemiluminescence (CL) emission when reacted with HO, which was more than 150 times that of ABEI functionalized MBs. Furthermore, it was found that 2,4,6-trinitrotoluene (TNT) aptamer could attach to the surface of Co/ABEI/MBs via electrostatic interaction and coordination interaction between TNT aptamer and Co, leading to a decrease in CL intensity due to the catalytic site Co being blocked by the aptamer. In the presence of TNT, TNT would bind strongly with TNT aptamer and detach from the surface of Co/ABEI/MBs, resulting in partial restoration of the CL signal. Accordingly, label-free aptasensor was developed for the determination of TNT in the range of 0.05-25 ng/mL with a detection limit of 17 pg/mL. This work demonstrates that Co/ABEI/MBs are easily connected with recognition biomolecules, which are not only magnetic carriers but also direct sensing interfaces with excellent CL activity. It provides a novel CL interface with a magnetic property which easily separates analytes from the sample matrix to construct label-free bioassays.

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mentioning

confidence: 99%

Highly Chemiluminescent Magnetic Beads for Label-Free Sensing of 2,4,6-Trinitrotoluene

Kong

Zhao²,

Zhu

et al. 2017

Anal. Chem.

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“…Similarly, Chang et al. () proposed a colorimetric immunoassay based on the photostimulation of chitosan‐modified AgI/TiO 2 heterojunction for highly sensitive chloramphenicol detection. Moreover, due to the nano size and stable properties, nanozymes are promising to be raw material for additive manufacturing applications such as 3D printing.…”

Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

“…Interestingly, Gong, Dai, Zhang, and Lin (2016) reported that the photoresponsive nanozyme of silver iodide-chitosan NPs could oxidize TMB under visible light illumination, which contributed to construction of a novel photocathodic immunosensor with largely enhanced sensitivity. Similarly, Chang et al (2017) proposed a colorimetric immunoassay based on the photostimulation of chitosan-modified AgI/TiO 2 heterojunction for highly sensitive chloramphenicol detection. Moreover, due to the nano size and stable properties, nanozymes are promising to be raw material for additive manufacturing applications such as 3D printing.…”

Section: Nanozyme As Multifunctional Sensing Elementmentioning

confidence: 99%

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

139

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The public concerns about agrifood safety call for innovative and reformative analytical techniques to meet the inspection requirements of high sensitivity, specificity, and reproducibility. Enzyme‐mimetic nanomaterials or nanozymes, which combine enzyme‐like properties with nanoscale features, emerge as an excellent tool for quality and safety detection in the agrifood sector, due to not only their robust capacity in detection but also their attraction in future‐oriented exploitations. However, in‐depth understanding about the fundamental principles of nanozymes for food quality and safety detection remains limited, which makes their applications largely empirical. This review provides a comprehensive overview of the principles, designs, and applications of nanozyme‐based detection technique in the agrifood industry. The discussion mainly involves three mimicking types, that is, peroxidase, oxidase, and catalase‐like nanozymes, capable of detecting major agrifood analytes. The current principles and strategies are classified and then discussed in details through discriminating the roles of nanozymes in diverse detection platforms. Thereafter, recent applications of nanozymes in detecting various endogenous ingredients and exogenous contaminants in foods are reviewed, and the outlook of profound developments are explained. Evidenced by the increasing publications, nanozyme‐based detection techniques are narrowing the gap to practical‐oriented food analytical methods, while some challenges in optimization of nanozymes, diversification of recognition‐to‐signal manners, and sustainability of methodology need to conquer in the future.

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“…Chitosan has benefits of natural availability as well as biocompatibility, brings it as a potential candidate to the creation of chitosan-based analytical tools for point-of-care clinical, biotechnological, and environmental analysis. Recently, the integration of chitosan with nanomaterials was reported as a peroxidase-like activity for visual sensing application [11,12]. However, up-to-now, no reports on bare chitosan as a peroxidase-like enzymatic activity has been published yet.…”

Section: Introductionmentioning

confidence: 99%

Chitozyme: First Peroxidase-like Activity of Chitosan for Multiplexed Visual Detection of H₂O₂, Glucose and Lactate on Paper-based Device

Ahmed

Weng

Neethirajan

2017

Preprint

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Visual read-out diagnostics tools are promising candidates for field applicable medical devices. Current colorimetric biosensors require introduction of natural enzymes or nanozymes, which has some serious drawbacks for practical applications. Chitosan, a natural polymer, provides safe and efficient compound in medical and pharmaceutical technology. Herein, we report on a simple, cost-efficient, field-portable, environmental friendly and ultra-sensitive multiplex detection platform based on peroxidase-like activity of chitosan in the presence of 3,3’,5,5’-Tetramethylbenzidine (TMBZ) and H2O2. This straight forward signal amplification strategy was successfully applied to detect H2O2, glucose and lactate with the limit of detection (LOD) of 2.64 pM, 0.104 μM and 2.8 nM respectively, represents the lowest LOD of H2O2, glucose and lactate with visual read-out. The chitosan-based assay performance was also retained in complex biological media for glucose and lactate detection. Furthermore, the proposed assay was successfully demonstrated as a paper-based colorimetric biosensor. Most importantly, the simplicity, biocompatibility and sensitivity of the proposed assay will open new doors for instrument free naked eye visual detection of H2O2, glucose and lactate detection.

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Photoresponsive colorimetric immunoassay based on chitosan modified AgI/TiO2 heterojunction for highly sensitive chloramphenicol detection

Cited by 70 publications

References 37 publications

Highly Chemiluminescent Magnetic Beads for Label-Free Sensing of 2,4,6-Trinitrotoluene

Highly Chemiluminescent Magnetic Beads for Label-Free Sensing of 2,4,6-Trinitrotoluene

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Chitozyme: First Peroxidase-like Activity of Chitosan for Multiplexed Visual Detection of H₂O₂, Glucose and Lactate on Paper-based Device

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Photoresponsive colorimetric immunoassay based on chitosan modified AgI/TiO2 heterojunction for highly sensitive chloramphenicol detection

Cited by 70 publications

References 37 publications

Highly Chemiluminescent Magnetic Beads for Label-Free Sensing of 2,4,6-Trinitrotoluene

Highly Chemiluminescent Magnetic Beads for Label-Free Sensing of 2,4,6-Trinitrotoluene

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Chitozyme: First Peroxidase-like Activity of Chitosan for Multiplexed Visual Detection of H2O2, Glucose and Lactate on Paper-based Device

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Product

Resources

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Chitozyme: First Peroxidase-like Activity of Chitosan for Multiplexed Visual Detection of H₂O₂, Glucose and Lactate on Paper-based Device