Photoregulation of Cellular Morphology during Complementary Chromatic Adaptation Requires Sensor-Kinase-Class Protein RcaE in
            <i>Fremyella diplosiphon</i>

Bordowitz, Juliana R.; Montgomery, Beronda L.

doi:10.1128/jb.00018-08

Cited by 69 publications

(142 citation statements)

References 29 publications

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“…The pigmentation and morphology of the F. diplosiphon strains used in this study when grown in replete media have been described in detail elsewhere (Bordowitz & Montgomery, 2008;Kehoe & Grossman, 1996). Cultures were grown at 28 uC with shaking under GL or RL as described previously (Bordowitz & Montgomery, 2008). GL (lmax 530 nm; Geneva Scientific) and RL (lmax 660 nm, 2506RD; LED Wholesalers) sources were identical to those detailed previously (Bordowitz & Montgomery, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we used F. diplosiphon strain SF33, a wild-type pigmentation strain that exhibits shortened filaments (Cobley et al, 1993), the photoreceptor mutant DrcaE (FdBk14; Kehoe & Grossman, 1996) and response regulator mutants DrcaF (FdR101; Bordowitz & Montgomery, 2008) and DrcaC (FdR102; Bordowitz & Montgomery, 2008). All mutant strains were derived in the SF33 background.…”

Section: Methodsmentioning

confidence: 99%

“…Cultures were grown at 28 uC with shaking under GL or RL as described previously (Bordowitz & Montgomery, 2008). GL (lmax 530 nm; Geneva Scientific) and RL (lmax 660 nm, 2506RD; LED Wholesalers) sources were identical to those detailed previously (Bordowitz & Montgomery, 2008). A Li-Cor light meter (model LI-250) connected to a Li-Cor quantum sensor (model LI-190SA) was used to measure light intensities.…”

Section: Methodsmentioning

confidence: 99%

“…All mutant strains were derived in the SF33 background. The pigmentation and morphology of the F. diplosiphon strains used in this study when grown in replete media have been described in detail elsewhere (Bordowitz & Montgomery, 2008;Kehoe & Grossman, 1996). Cultures were grown at 28 uC with shaking under GL or RL as described previously (Bordowitz & Montgomery, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…CCA is regulated by the photoreceptor RcaE and its downstream response regulators RcaF and RcaC, particularly in response to green light (GL) and red light (RL) (reviewed by Gutu & Kehoe, 2012;Kehoe & Gutu, 2006). Upon mutation of the genes encoding these proteins, the colour phenotype (Kehoe & Grossman, 1996, as well as the morphology of cells, is altered (Bordowitz & Montgomery, 2008;. During CCA in F. diplosiphon, genes whose expression levels were regulated by light were implicated in iron-related processes (StoweEvans et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Responses to iron limitation are impacted by light quality and regulated by RcaE in the chromatically acclimating cyanobacterium Fremyella diplosiphon

Pattanaik

Busch

et al. 2014

Microbiology

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Photosynthetic organisms adapt to environmental fluctuations of light and nutrient availability. Iron is critical for photosynthetic organismal growth, as many cellular processes depend upon iron cofactors. Whereas low iron levels can have deleterious effects, excess iron can lead to damage, as iron is a reactive metal that can result in the production of damaging radicals. Therefore, organisms regulate cellular iron levels to maintain optimal iron homeostasis. In particular, iron is an essential factor for the function of photosystems associated with photosynthetic light-harvesting complexes. Photosynthetic organisms, including cyanobacteria, generally respond to iron deficiency by reduced growth, degradation of non-essential proteins and in some cases alterations of cellular morphology. In response to fluctuations in ambient light quality, the cyanobacterium Fremyella diplosiphon undergoes complementary chromatic adaptation (CCA). During CCA, phycobiliprotein composition of light-harvesting antennae is altered in response to green light (GL) and red light (RL) for efficient utilization of light energy for photosynthesis. We observed light-regulated responses to iron limitation in F. diplosiphon. RL-grown cells exhibited significant reductions in growth and pigment levels, and alterations in iron-associated proteins, which impact the accumulation of reactive oxygen species under iron-limiting conditions, whereas GL-grown cells exhibited partial resistance to iron limitation. We investigated the roles of known CCA regulators RcaE, RcaF and RcaC in this light-dependent iron-acclimation response. Through comparative analyses of wild-type and CCA mutant strains, we determined that photoreceptor RcaE has a central role in light-induced oxidative stress associated with iron limitation, and impacts light-regulated iron-acclimation responses, physiologically and morphologically.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Responses to iron limitation are impacted by light quality and regulated by RcaE in the chromatically acclimating cyanobacterium Fremyella diplosiphon

Pattanaik

Busch

et al. 2014

Microbiology

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Regulation of biofilm formation and cellular buoyancy through modulating intracellular cyclic di‐GMP levels in engineered cyanobacteria

Agostoni

Waters

Montgomery

2015

Biotech & Bioengineering

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The second messenger cyclic dimeric (3'→5') GMP (cyclic di-GMP or c-di-GMP) has been implicated in the transition between motile and sessile lifestyles in bacteria. In this study, we demonstrate that biofilm formation, cellular aggregation or flocculation, and cellular buoyancy are under the control of c-di-GMP in Synechocystis sp. PCC 6803 (Synechocystis) and Fremyella diplosiphon. Synechocystis is a unicellular cyanobacterium and displays lower levels of c-di-GMP; F. diplosiphon is filamentous and displays higher intracellular c-di-GMP levels. We transformed Synechocystis and F. diplosiphon with a plasmid for constitutive expression of genes encoding diguanylate cylase (DGC) and phosphodiesterase (PDE) proteins from Vibrio cholerae or Escherichia coli, respectively. These engineered strains allowed us to modulate intracellular c-di-GMP levels. Biofilm formation and cellular deposition were induced in the DGC-expressing Synechocystis strain which exhibited high intracellular levels of c-di-GMP; whereas strains expressing PDE in Synechocystis and F. diplosiphon to drive low intracellular levels of c-di-GMP exhibited enhanced cellular buoyancy. In addition, the PDE-expressing F. diplosiphon strain showed elevated chlorophyll levels. These results imply roles for coordinating c-di-GMP homeostasis in regulating native cyanobacterial phenotypes. Engineering exogenous DGC or PDE proteins to regulate intracellular c-di-GMP levels represents an effective tool for uncovering cryptic phenotypes or modulating phenotypes in cyanobacteria for practical applications in biotechnology applicable in photobioreactors and in green biotechnologies, such as energy-efficient harvesting of cellular biomass or the treatment of metal-containing wastewaters.

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Gene Manipulation and Biosynthesis of Phycobiliproteins

Kannaujiya

Sundaram

Sinha

2017

Phycobiliproteins: Recent Developments and Future Applications

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Photoregulation of Cellular Morphology during Complementary Chromatic Adaptation Requires Sensor-Kinase-Class Protein RcaE in Fremyella diplosiphon

Cited by 69 publications

References 29 publications

Responses to iron limitation are impacted by light quality and regulated by RcaE in the chromatically acclimating cyanobacterium Fremyella diplosiphon

Responses to iron limitation are impacted by light quality and regulated by RcaE in the chromatically acclimating cyanobacterium Fremyella diplosiphon

Regulation of biofilm formation and cellular buoyancy through modulating intracellular cyclic di‐GMP levels in engineered cyanobacteria

Gene Manipulation and Biosynthesis of Phycobiliproteins

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