Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center

Fang, Yuan; Liu, Dongyang; Jiang, Jingjing; He, Axin; Zhu, Rui; Tian, Lijin

doi:10.1016/j.jbc.2022.101783

Cited by 6 publications

(5 citation statements)

References 69 publications

(111 reference statements)

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“…The acclimation properties identified here may extend to additional PBS system functioning under different light regimes. Pigmented linkers, which are responsible for several of the close chromophore pairs in the structure, are present in marine Synechococcus species 22 , 54 . These open ocean organisms also have the ability to manipulate their PBS cross-section 40 .…”

Section: Resultsmentioning

confidence: 99%

“…Its extensive spectral range results from the chromophore composition of its three protein classes: phycoerythrin (PE), which absorbs at ~493 nm and fluoresces at ~574 nm, phycocyanin (PC), which absorbs at 620 nm and fluoresces at 650 nm, and allophycocyanin (APC), which absorbs at 650 nm and fluoresces at ~660 nm 3 . The PBS pigment composition may vary and additional outlier cases have been reported 22 , 23 . The ratio between these classes determines the effective cross-section of the PBS.…”

Section: Introductionmentioning

confidence: 99%

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The structural basis for light acclimation in phycobilisome light harvesting systems systems in Porphyridium purpureum

Dodson,

Ma,

Suissa Szlejf

et al. 2023

Commun Biol

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Photosynthetic organisms adapt to changing light conditions by manipulating their light harvesting complexes. Biophysical, biochemical, physiological and genetic aspects of these processes are studied extensively. The structural basis for these studies is lacking. In this study we address this gap in knowledge by focusing on phycobilisomes (PBS), which are large structures found in cyanobacteria and red algae. In this study we focus on the phycobilisomes (PBS), which are large structures found in cyanobacteria and red algae. Specifically, we examine red algae (Porphyridium purpureum) grown under a low light intensity (LL) and a medium light intensity (ML). Using cryo-electron microscopy, we resolve the structure of ML-PBS and compare it to the LL-PBS structure. The ML-PBS is 13.6 MDa, while the LL-PBS is larger (14.7 MDa). The LL-PBS structure have a higher number of closely coupled chromophore pairs, potentially the source of the red shifted fluorescence emission from LL-PBS. Interestingly, these differences do not significantly affect fluorescence kinetics parameters. This indicates that PBS systems can maintain similar fluorescence quantum yields despite an increase in LL-PBS chromophore numbers. These findings provide a structural basis to the processes by which photosynthetic organisms adapt to changing light conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The structural basis for light acclimation in phycobilisome light harvesting systems systems in Porphyridium purpureum

Dodson,

Ma,

Suissa Szlejf

et al. 2023

Commun Biol

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“…Б -зависимость обобщённого параметра A от интенсивности света. Линия -аппроксимация выражением (17).…”

Section: математическая модельunclassified

“…Известно, что все водоросли содержат внутримембранные LHC, ассоциированные как с PSI, так и с PSII. Среди морских водорослей выделяют три основных типа LHC: 1 -у зелёных водорослей они представляют собой белковые комплексы, связанные с хлорофиллами a и b и ксантофиллами, в основном лютеином; 2 -LHC бурых водорослей содержат очень много фукоксантина наряду с хлорофиллами a и c; 3 -LHC красных водорослей, которые содержат хлорофилл a и зеаксантин, LHC ассоциированы только с РЦ 1 [13][14][15][16][17]. У цианобактерии Arthrospira platensis хлорофилл а входит в структуру LHC: на одну молекулу реакционного центра в накопительной культуре в зависимости от интенсивности света приходится 120-180 молекул антенного хлорофилл а, при этом 95 % хлорофилла связано с первой фотосистемой [18].…”

Section: Introductionunclassified

“…У цианобактерии Arthrospira platensis хлорофилл а входит в структуру LHC: на одну молекулу реакционного центра в накопительной культуре в зависимости от интенсивности света приходится 120-180 молекул антенного хлорофилл а, при этом 95 % хлорофилла связано с первой фотосистемой [18]. Аналогичная картина характерна и для красных микроводорослей, в частности, у P. purpureum почти весь хлорофилл а связан с LHC первой фотосистемы, а антенна второй фотосистемы представлена ФБП [17].…”

Section: Introductionunclassified

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Modeling Growth and Photoadaptation of Porphyridium purpureum Batch Culture

Lelekov,

Klochkova

2024

Math.Biol.Bioinf.

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The work focuses on mathematical modeling of linear growth marine red algae Porphyridium purpureum batch culture under various surface irradiation. Before starting the batch culture P. purpureum was maintained for 3–5 days in turbidostat, which allowed the culture to adapt to a given light intensity. On each batch curve the maximum specific growth rate and maximum productivity are determined by approximating the exponential and linear growth phases with the appropriate equations. It is taken into account that at the beginning of the linear growth phase the integral light absorption coefficient, calculated from the true absorption spectrum in PAR range, exceeded 50 %. It is shown that with light intensity increase from 15 to 227 µmol photons m–2·s–1 the maximum specific growth rate increased from 0.31 to 1 day–1, the maximum productivity increased from 1.32 to 16.38 g DW m–2·day–1. Mathematical modeling of the linear growth of P. purpureum batch culture have showed that at any light intensity the specific growth rate is determined by the surface illumination, the light absorption coefficient and chlorophyll a concentration. The concept of reduced irradiation – the amount of absorbed light energy per chlorophyll a – was introduced. A linear dependence of the specific growth rate on reduced irradiation is given. The tangent of the angle of line slope is determined by the organization of the key multi-enzyme complex («metabolism bottleneck»). Parameters of this complex depend on the cells photoadaptation degree. For the first time a quantitative relationship between multi-enzyme complex parameters, light intensity and the chlorophyll / P700 ratio was established.

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Identification of multiple nonphotochemical quenching processes in the extremophilic red alga Cyanidioschyzon merolae

Chiang

Huang

2022

Photosynth Res

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Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center

Cited by 6 publications

References 69 publications

The structural basis for light acclimation in phycobilisome light harvesting systems systems in Porphyridium purpureum

The structural basis for light acclimation in phycobilisome light harvesting systems systems in Porphyridium purpureum

Modeling Growth and Photoadaptation of Porphyridium purpureum Batch Culture

Identification of multiple nonphotochemical quenching processes in the extremophilic red alga Cyanidioschyzon merolae

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