Photoprotective Effects of Topical Formulations Containing a Combination of <i>Ginkgo biloba</i> and Green Tea Extracts

Belo, Susi Elaine Dal; Gaspar, Lorena Rigo; Campos, Patrícia Maria Berardo Gonçalves Maia

doi:10.1002/ptr.3507

Cited by 40 publications

(22 citation statements)

References 30 publications

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“…In an artificial skin culture model GTC decreased the level of MMPs production and increased TIMP-1 expression level, and reduced alterations in the extracellular matrix following application of UVA radiation [ 25 ]. A comparable UVA/UVB radiation study undertaken in a mouse model using EGCG and ginkgo biloba showed that the two extracts had a complimentary photoprotective effect, linked to a biological/free radical scavenging effect as opposed to a UV-filtering effect [ 7 ]. GTCs also play a role in angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Functional testing of topical skin formulations using an optimised ex vivo skin organ culture model

Sidgwick

McGeorge²

2016

Arch Dermatol Res

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A number of equivalent-skin models are available for investigation of the ex vivo effect of topical application of drugs and cosmaceuticals onto skin, however many have their drawbacks. With the March 2013 ban on animal models for cosmetic testing of products or ingredients for sale in the EU, their utility for testing toxicity and effect on skin becomes more relevant. The aim of this study was to demonstrate proof of principle that altered expression of key gene and protein markers could be quantified in an optimised whole tissue biopsy culture model. Topical formulations containing green tea catechins (GTC) were investigated in a skin biopsy culture model (n = 11). Punch biopsies were harvested at 3, 7 and 10 days, and analysed using qRT-PCR, histology and HPLC to determine gene and protein expression, and transdermal delivery of compounds of interest. Reduced gene expression of α-SMA, fibronectin, mast cell tryptase, mast cell chymase, TGF-β1, CTGF and PAI-1 was observed after 7 and 10 days compared with treated controls (p < 0.05). Histological analysis indicated a reduction in mast cell tryptase and chymase positive cell numbers in treated biopsies compared with untreated controls at day 7 and day 10 (p < 0.05). Determination of transdermal uptake indicated that GTCs were detected in the biopsies. This model could be adapted to study a range of different topical formulations in both normal and diseased skin, negating the requirement for animal models in this context, prior to study in a clinical trial environment.

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Section: Discussionmentioning

confidence: 99%

Functional testing of topical skin formulations using an optimised ex vivo skin organ culture model

Sidgwick

McGeorge²

2016

Arch Dermatol Res

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“…However, most tests have been conducted by oral ingestion or topical application of these ingredients in inappropriate vehicles, which can alter the skin barrier. To be of practical use to dermatologists and to be safe and efficient, extracts must have physicochemical characteristics that permit their incorporation in adequate concentrations in a skin-compatible vehicle (e.g., gels, moisturizing creams) [34]. In this study, the capacity of the Morinda citrifolia extract, added to topical formulations (gels), to prevent or to treat UVA-UVB irradiation-induced skin damage in mice was evaluated.…”

Section: Discussionmentioning

confidence: 99%

UVA-UVB Photoprotective Activity of Topical Formulations ContainingMorinda citrifoliaExtract

Serafini

Detoni

Menezes

et al. 2014

BioMed Research International

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Exposure to solar radiation, particularly its ultraviolet (UV) component, has a variety of harmful effects on human health. Some of these effects include sunburn cell formations, basal and squamous cell cancers, melanoma, cataracts, photoaging of the skin, and immune suppression. The beneficial photoprotective effects of topical formulations with the extract, Morinda citrifolia, have not been investigated. This present study aims to investigate the potential benefits of M. citrifolia topical application on the dorsal skin of mice, exposed to UVA-UVB light. Using 7 days of treatment, [before (baseline values) and 20 h after UV exposure], the thickness, skin barrier damage (TEWL), erythema, and histological alterations were evaluated. The results showed that the formulations containing the extract protected the skin against UV-induced damage.

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“… 10 The Ginkgo fruits (nuts) are also known to have health benefits for prevention of cancer, cardiovascular, neurological disorder, and photoprotective effect. 11 , 12 …”

Section: Introductionmentioning

confidence: 99%

In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract

Ghimeray¹,

Jung²,

Lee³

et al. 2015

CCID

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BackgroundIn phytotherapy, the therapeutic potential is based on the combined action of different herbal drugs. Our objective was to evaluate the antioxidant, anti-collagenase (in vitro), and anti-wrinkle (in vivo) effect of combined formulation containing Ginkgo biloba, Punica granatum, Ficus carica, and Morus alba fruits extract.MethodsAntioxidant evaluation was based on the scavenging activity of free radicals (1,1-diphenyl-2-picrylhydrazyl, H2O2, and O2−) and the anti-collagenase activity was based on the reduction of collagenase enzyme in vitro. In an in vivo study, 21 female subjects were examined in a placebo-controlled trail. Facial wrinkle, especially the crow’s feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo.ResultsIn the in vitro study, the combination of fruits extract showed a higher antioxidant activity which was comparable with the positive standard (ascorbic acid, butylated hydroxyanisole, and Trolox). The data also showed a dose-dependent inhibition of collagenase. In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively.ConclusionThe combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin.

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Photoprotective Effects of Topical Formulations Containing a Combination of Ginkgo biloba and Green Tea Extracts

Cited by 40 publications

References 30 publications

Functional testing of topical skin formulations using an optimised ex vivo skin organ culture model

Functional testing of topical skin formulations using an optimised ex vivo skin organ culture model

UVA-UVB Photoprotective Activity of Topical Formulations ContainingMorinda citrifoliaExtract

In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract

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