Photoprotective effect of vitamins A and E on polyamine and oxygenated free radical metabolism in hairless mouse epidermis

Khettab, Nadine; Amory, Marie-Claude; Briand, G; Bousquet, Bernard; Combre, A; Forlot, P.; Barey, Marc

doi:10.1016/0300-9084(88)90028-4

Cited by 37 publications

(20 citation statements)

References 14 publications

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“…Polyamines are multifunctional and involved in cell proliferation, cell survival, cell signaling, and apoptosis (37). Khettab et al (38) showed a significant increase in the production of free radicals and polyamine biosynthesis in the epidermis of mice after UV, suggesting one potential mechanism for this up-regulation of polyamines in sun-exposed skin, although elevated polyamine concentrations and ODC activity have been implicated in skin tumorigenesis (39,40). O'Brien et al (39) showed that in transgenic mice, overexpression of ODC in skin was associated with elevated tissue polyamines, particularly putrescine and the development and maintenance of the neoplastic phenotype (40).…”

Section: Discussionmentioning

confidence: 99%

Reproducibility and Expression of Skin Biomarkers in Sun-Damaged Skin and Actinic Keratoses

Einspahr

Warneke³

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Objectives: To explore p53 and proliferating cell nuclear antigen (PCNA) expression and polyamine content as biomarkers in skin cancer chemoprevention trials, we evaluated their expression in early stages of UV-induced squamous cell tumorigenesis. Methods: Biopsies were collected from three groups: 78 subjects with sun damage on forearms, 33 with actinic keratosis (AK) on forearms, and 32 with previous squamous cell carcinoma. Participants with sun damage were randomized to sunscreen or no sunscreen. Results: We found significant differences in p53 and polyamines in forearms from the sun-damaged group (11.5 F 1.2% for p53, 65.5 F 1.9 nmol/g for putrescine, and 187.7 F 3.3 nmol/g for spermidine) compared with the group with sun damage plus AK (20.9 F 2.3% for p53, P = 0.0001; 81.7 F 3.9 nmol/g for putrescine, P = 0.0001; 209.4 F 8.2 nmol/g for spermidine, P < 0.06). PCNA was not different. When lesion histology was considered, there was a stepwise significant increase in p53 in biopsies without characteristics of AK compared with early AK (P = 0.02) and AK (P = 0.0006) and a similar pattern for PCNA with the only significant difference between early AK and AK. There was a stepwise increase in putrescine and spermidine in normal, sun-damaged forearm, forearm from subjects with AK, and the AK lesion itself (P < 0.0001). No significant differences in p53 or polyamines were seen in 3-month biopsies or, as a result of sunscreen use, although PCNA in the sun-damaged group not using sunscreen decreased significantly. Conclusions: p53 expression and polyamines in skin were elevated in early stages of skin tumorigenesis and were not affected by sunscreen, adding validity to their use as biomarkers in skin cancer chemoprevention trials. (Cancer Epidemiol Biomarkers Prev 2006;15(10):1841 -8)

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Section: Discussionmentioning

confidence: 99%

Reproducibility and Expression of Skin Biomarkers in Sun-Damaged Skin and Actinic Keratoses

Einspahr

Warneke³

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Numerous topical studies have demonstrated that vitamin E application prior to ultraviolet exposure significantly reduces acute skin responses, such as erythema and edema, sunburn cell formation (Darr et al, 1996;Lin et al, 2003;Ritter et al, 1997), lipid peroxidation (Khettab et al, 1988;Lopez-Torres et al, 1998;Yuen and Halliday, 1997), DNA-adduct formation, immunosuppression (Gensler and Magdaleno, 1991;Yuen and Halliday, 1997), as well as UVA-induced binding of photosensitizers (Beijersbergen van Henegouwen et Schoonderwoerd et al, 1991) and chemiluminescence (Evelson et al, 1997). Chronic skin reactions due to prolonged UVB/UVA-exposure, such as skin wrinkling (Bissett et al, 1990(Bissett et al, , 1992(Bissett et al, , 1989Jurkiewicz et al, 1995), and skin tumor incidence (Bissett et al, 1990(Bissett et al, , 1992(Bissett et al, , 1989Burke et al, 2000;Gensler and Magdaleno, 1991) were also diminished by topical vitamin E formulations.…”

Section: Photoprotection Provided By Vitamin Ementioning

confidence: 98%

Vitamin E in human skin: Organ-specific physiology and considerations for its use in dermatology

Thiele

Ekanayake‐Mudiyanselage

2007

Molecular Aspects of Medicine

156

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“…Retinoic acid (RA) is highly lipophilic (logP = 4.6) and the most potent of the retinoids. It exerts a wide variety of effects in the regulation of cell division, and has some antioxidant properties [1,6]. RA contains a carboxylic acid group, whereas AsA contains hydroxyl/ene-ol functionality, thus allowing the formation of an ester co-drug.…”

Section: Introductionmentioning

confidence: 99%

“…UV-mediated oxidative damage in the skin may be controlled by the topical application of antioxidants [1,2]. L-Ascorbic acid (AsA) is a powerful water-soluble antioxidant that efficiently protects important organic and biological molecules against oxidative degradation by inhibiting free radical-initiated lipid peroxidation, a process believed to be responsible for photoaging and the induction of collagen synthesis [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…These were: (1) human callus (HK) shavings, shown by some researchers to be a suitable substrate to simulate and quantify solute uptake into human skin [25,26], (2) protein extracted from PESK, as PESK is often used as a substitute for human skin and (3) purified bovine horn keratin (BHK), sometimes used as model keratin substitute for epidermal keratin [8,9,17,18]. Also included were AsA and RA for comparison, along with retinol (Rol), retinyl palmitate (Rol-Pal), and ascorbyl palmitate (AsA-Pal), which are currently used in topical formulations for cosmetic and medicinal purposes [1,3,4,6,27]. The structures of the test compounds are shown in figure 1.…”

Section: Introductionmentioning

confidence: 99%

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Topical Delivery of Retinyl Ascorbate Co-Drug

Abdulmajed

Heard

McGuigan

et al. 2004

Skin Pharmacol Physiol

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Retinyl ascorbate (RA-AsA), an ester co-drug of vitamins A (RA) and C (AsA), is proposed as a topical antioxidant/cell division regulator for reducing UV-induced generation of free radicals and disrupted dermal cell growth. The efficacy of dermatological agents is influenced by their retention within the skin, which is increased by the interaction with skin components. Keratin is the major protein (∼95%) in the skin, and this paper reports the binding of RA-AsA, RA, AsA, retinol, ascorbic acid palmitate and retinol palmitate to three tissues – human callus, pig ear skin and bovine horn keratin. Tissue samples were incubated with solutions of compounds and the uptake measured as the ratio of bound/free compound at equilibrium. Binding to keratin was assessed using delipidised tissue, and was much higher for the polar compounds, suggesting dipolar/H-bonding interaction. Binding strength was ranked as human > porcine > bovine, but there was no distinction for highly lipophilic compounds. The binding characteristic of native tissues was complicated by lipid content of the tissues. There seemed to be a dual effect. The binding of very lipophilic materials increased with lipid content, implying that a substantial amount is dissolved in the lipid matrix. For highly polar AsA, lipid content decreased the binding, suggesting that the lipid reduced the strong polar interactions with skin protein/keratin.

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Photoprotective effect of vitamins A and E on polyamine and oxygenated free radical metabolism in hairless mouse epidermis

Cited by 37 publications

References 14 publications

Reproducibility and Expression of Skin Biomarkers in Sun-Damaged Skin and Actinic Keratoses

Reproducibility and Expression of Skin Biomarkers in Sun-Damaged Skin and Actinic Keratoses

Vitamin E in human skin: Organ-specific physiology and considerations for its use in dermatology

Topical Delivery of Retinyl Ascorbate Co-Drug

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