Photophysics and photochemistry of rose bengal bound to human serum albumin

Alarcon, Emilio I.; Edwards, A.; Aspée, Alexis; Borsarelli, Claudio D.; Lissi, E. A.

doi:10.1039/b901056d

Cited by 65 publications

(79 citation statements)

References 59 publications

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“…Likewise, Rose Bengal, one of the frequently used photosensitizers due to its high water solubility, high singlet oxygen quantum yield and low rate of photodegradation [76], is used in silica nanoparticles to inactivate gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and showed high efficiency in inactivating bacterial through photodynamic action [77]. Related studies on methylene blue loaded nanoparticles have also been reported [78,79].…”

Section: Ps Linkermentioning

confidence: 99%

Nanodrug applications in photodynamic therapy

Paszko

Ehrhardt

Senge

et al. 2011

Photodiagnosis and Photodynamic Therapy

319

210

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SummaryPhotodynamic therapy (PDT) has developed over last century and is now becoming a more widely used medical tool having gained regulatory approval for the treatment of various diseases such as cancer and macular degeneration. It is a two-step technique in which delivery of a photosensitizing drug is followed by irradiation of light. Activated photosensitizers transfer energy to molecular oxygen which results in generation of reactive oxygen species which in turn cause cells apoptosis or necrosis. Although this modality has significantly improved quality of life and survival time for many cancer patients it still offers significant potential for further improvement. In addition to the development of new PDT drugs, the use of nanosized carriers for photosensitizers is a promising approach which might improve the efficiency of photodynamic activity and which can overcome many side effects associated with classic photodynamic therapy. This review aims at highlighting the different types of nanomedical approaches currently used in PDT and outlines future trends and limitations of nanodelivery of photosensitizers. PDT -photodynamic therapy; PGA -poly(glycolic acid); PGLA -poly(D,L-lactide-coglycolide); PLA -poly(lactic acid); PS -photosensitizer; PEG -polyethylene glycol; ALA  aminolevulinic acid; m-THPC  5,10,15,20-tetra-(m-hydroxyphenyl)chlorine; m-THPP  meso-tetra(p-hydroxyphenyl); PpIX  protoporphyrin; Hp  hematoporphyrin; Pc4  silicon phthalocyanine; Ig  immunoglobulin; Tf  transferrin; TfR  transferrin receptor; VEGF  vascular endothelial growth factor; EPR  enhanced permeability and retention effect; FRET  fluorescence resonance energy transfer; MRI  magnetic resonance imaging; RES  reticuloendothelial system; ROS  reactive oxygen species; NP  nanoparticle; ND -nanodiamonds; SNP  silica nanoparticle; AMD  age-related macular degeneration; CNV  choroidal neovascularization; PTT  photothermal therapy;

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Section: Ps Linkermentioning

confidence: 99%

Nanodrug applications in photodynamic therapy

Paszko

Ehrhardt

Senge

et al. 2011

Photodiagnosis and Photodynamic Therapy

319

210

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“…Metal nanoparticles based sensors are used for highly sensitive biomedical applications now-a-days. Fluorescence based sensing and binding can provide information about the micro environment of the protein, nanoparticles and sugar molecules [23][24][25][26][27][28][29][30][31][32]. Time resolved fluorescence measurement is a sophisticated technique to sense even a small change in protein, nanoparticles and sugar molecular interactions.…”

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confidence: 99%

Fluorescence Quenching of b-Casein by Silver Nanoparticles and Copper Nanoparticles in Presence of Sugars: A Quantitative Exploration

Vasumathi¹,

Santhanalakshmi²

2018

Asian J. Chem.

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INTRODUCTIONInvention of non-invasive in vivo biosensors is today's necessity in diagnostics, medical analysis and therapy [1,2]. Detection and estimation of sugars in biological systems attract the attention of medical engineers. When protein-carbohydrate interactions are studied and established in presence of silver and copper nanoparticles, new immunotherapeutic strategies may be discovered in the treatment of malignant diseases [3]. In this paper the studies on the interaction of silver nanoparticles (AgNP) and copper nanoparticles (CuNP) with milk protein, β-casein (BCA) is quantitatively described using fluorescence quenching measurements. The interaction studies between the protein and the nanoparticles (Pro-NP) are extensively studied in presence of various monosaccharide and disaccharide sugars like glucose, fructose, galactose, sucrose, maltose and lactose. The protein β-casein (BCA) exhibits a strong fluorescence emission, which is sensitive to active quenchers. Metal nanoparticles like copper nanoparticles and silver nanoparticles with enhanced and unique properties are reported to quench protein fluorescence appreciably. In the present work, the fluorescence quenching effects of metal nanoparticles on the protein fluorescence are extensively studied in presence of various sugar molecules. This may play a significant role in the sensing applications of sugar molecular systems in the presence of metal nanoparticles. β-Casein is used as fluorophore and sugar molecular systems like sucrose, maltose, fructose, lactose, galactose and glucose are chosen as quenchers and the protein-sugar interactions are arrived from fluorescence quenching results in the presence of copper nanoparticles and silver nanoparticles. To find the role of life time on the intensity of fluorescence, time resolved fluorescence measurements for the protein-sugar systems in the presence and absence of nanoparticles is measured. The proteinnanoparticles-sugar fluorescence interactions are analyzed by using Stern-Volmer plots and thermodynamic plots using fluorescence intensity quenching data. The trends in the extent of quenching action on protein fluorescence and also the binding interactions among protein sugar systems are quantified in the presence of copper nanoparticles and silver nanoparticles separately, in terms of Stern-Volmer constant values and binding constants values. β-Casein is found to interact strongly with the quenchers in the presence of copper nanoparticle and silver nanoparticle. Silver nanoparticle is read to be a better quenching mediator than copper nanoparticle for the fluorescence intensity in molecular interactions. Order of sugars quenching the protein fluorescence in presence of the nanoparticles is found.

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“…Furthermore, certain food colorants, including rose bengal, serve as effective singlet oxygen generators or pro-oxidants of lipids when the peroxide value is measured as an oxidation index. 15,18) However, the fluorescein-type colorants indeed showed an antioxidative effect on differentiated HL-60 cells in the dark condition with comparable activity to that of the well-known naturally occurring pigments already discussed. Such antioxidants as (À)-epigallocatechin-3-gallate are known to have a pro-oxidative effect depending on the conditions 19) and biological activity through the oxidative reaction.…”

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confidence: 99%

“…However, a negative control group without the dye showed no fluorescence peak. Since rose bengal has been reported to readily bind to human serum albumin, 15) the effect of FBS or BSA on rose bengal incorporation was examined. As expected, the cellular incorporation was significantly decreased by pretreating with FBS as well as BSA (Fig.…”

mentioning

confidence: 99%