Photophysical study of the interaction between ZnO nanoparticles and globular protein bovine serum albumin in solution and in a layer-by-layer self-assembled film

Hansda, Chaitali; Maiti, Pradip; Singha, Tanmoy; Pal, Manisha; Hussain, Syed Arshad; Paul, Sharmistha; Paul, Piyali

doi:10.1016/j.jpcs.2018.05.029

Cited by 12 publications

(8 citation statements)

References 72 publications

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“…Quenching of the fluorescence of BSA due to interaction with ZnO NP through the formation of stable ground state complexes was also observed [12], highlighting the fact that both ZnO NP and BSA can influence each other's properties and it is not solely the action of one or the other. Such complex interaction could be occurring in our case, where biomolecules contained in MHB may coat ZnO-NTP, forming a protein/carbohydrate corona that results in PL emission quenching.…”

Section: Discussionmentioning

confidence: 84%

Plasma-synthesised Zinc oxide nanoparticle behavior in liquids

Rutherford

Jíra

Kolářová

et al. 2021

NANOCON 2021 Conference Proeedings

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Zinc oxide nanoparticles have been synthesized using non-thermal atmospheric pressure plasma (ZnO-NTP). We investigated the behavior of these ligand-free as a colloid suspension using different solvents, from deionized water to physiological saline and microbial culture broth. We found that the zeta potential of ZnO-NTP became more negative after exposure to microbial culture broth relative to water, which suggests increased colloid stability. Photoluminescence spectra of ZnO-NTP were similar regardless of liquid type, yet optical and fluorescent images of samples showed different agglomeration behavior depending on liquid type. Scanning electron microscopy images revealed large agglomerates of ZnO-NTP interacting with the surface of bacteria cells, ranging in size from 200 nm up to 2 µm. We also studied effect of sub-lethal concentrations of ZnO-NTP on bacteria under illumination. There was no significant difference in viable bacteria concentration after 24h exposure to 10 µg/mL ZnO-NTP relative to untreated control irrespective of sample illumination.

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Section: Discussionmentioning

confidence: 84%

Plasma-synthesised Zinc oxide nanoparticle behavior in liquids

Rutherford

Jíra

Kolářová

et al. 2021

NANOCON 2021 Conference Proeedings

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“…This allows the barbed fibers to quickly adsorb a large amount of BSA. Hansda et al confirmed that the alternate multilayer growth of the ZnO/BSA layer-by-layer film could be deposited via the electrostatic interactions, and the BSA molecular domains adsorbed on the ZnO surface may have the self-association effect. Therefore, we speculate that due to the bulk effect of a large amount of BSA, the protein on the ZnO surface may be desorbed in avalanche style.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Barbed ZnO Fibers and the Selective Adsorption Behavior for BSA

Liu

Dai

2021

ACS Omega

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ZnO electrospun nanofibers can act as seed fibers to fabricate multidentate barbed fibers perpendicular to the growth of the fibers using the chemical bath deposition (CBD) method. Fibers with a multirod morphology have a porous grid structure. The sample is easy to recover, and the nonpolar surface in the sample is sufficiently exposed. In the research of barbed fiber fabrication and adsorption on bovine serum albumin (BSA), the effects of different chemical bath conditions on the growth of ZnO nanorods were discussed. Barbed fibers with large slenderness ratios were obtained at a water content of 60 mL at 75 °C. Each milligram of barbed fibers can quickly adsorb about 162 μg of protein within 30 min. The adsorption activity of BSA between polar and nonpolar ZnO surfaces was also studied. The selective adsorption behavior of BSA on the nonpolar surface was revealed.

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“…[ 24 ] Through biexponential fitting, the average lifetime of complexes 1–7 interacting with BSA system can be calculated by Formula . [ 25 ]…”

Section: Resultsmentioning

confidence: 99%

Synthesis, characterization, superoxide dismutase, and antimicrobial activities of Co (II) complexes with Schiff base ligand and their hybrid proteins

Song

et al. 2022

Applied Organom Chemis

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Metal complexes have been reported to possess excellent free radical scavenging and antimicrobial properties. In the present study, seven linear trinuclear complexes were synthesized based on bis Schiff base ligand and complexes 1, 3–7 are reported for the first time. Their structure was characterized by IR spectroscopy and single crystal diffraction. BSA was used as carrier to hybridize the synthesized complexes to construct hybrid proteins. The interaction between metal complex and BSA was studied by UV–Vis spectrophotometry and fluorescence spectroscopy. Static quenching was the main mechanism identified for the interaction between metal complex and BSA. In the superoxide dismutase (SOD) activity test, IC50 values of complexes and hybrid proteins range between 2.89 and 26.53 μM, with hybrid proteins demonstrating a stronger activity than the complexes. Among all the complexes studied, complex 5 exhibited the strongest inhibition against Candida albicans (MIC 12.5 μg ml−1) and Cryptococcus neoformans (MIC 6.5 μg ml−1).

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Photophysical study of the interaction between ZnO nanoparticles and globular protein bovine serum albumin in solution and in a layer-by-layer self-assembled film

Cited by 12 publications

References 72 publications

Plasma-synthesised Zinc oxide nanoparticle behavior in liquids

Plasma-synthesised Zinc oxide nanoparticle behavior in liquids

Preparation of Barbed ZnO Fibers and the Selective Adsorption Behavior for BSA

Synthesis, characterization, superoxide dismutase, and antimicrobial activities of Co (II) complexes with Schiff base ligand and their hybrid proteins

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