Photophysical studies on curcumin-sophorolipid nanostructures: applications in quorum quenching and imaging

Vasudevan, Sahana; Prabhune, Asmita

doi:10.1098/rsos.170865

Cited by 29 publications

(18 citation statements)

References 67 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within the nanodisc environment, we also saw increased solubility over curcumin solubilized in water and a similar solubility to curcumin solubilized in DMSO (curDMSO) ( Figure 2 C). Contrary to the yellow fluorescence observed under UV light for curDMSO solutions, our curcumin nanodiscs demonstrate a slight blue shift in fluorescence, indicating incorporation of the curcumin into the lipid nanodisc environment ( Figure 2 C), as has been described previously [ 30 ]. Across three biological replicates, our cNLP formulation yielded an average of 348 µg/mL incorporated curcumin.…”

Section: Resultssupporting

confidence: 80%

Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner

et al. 2022

View full text Add to dashboard Cite

Curcumin, a natural polyphenol derived from the spice turmeric (Curcuma longa), contains antioxidant, anti-inflammatory, and anti-cancer properties. However, curcumin bioavailability is inherently low due to poor water solubility and rapid metabolism. Here, we further refined for use curcumin incorporated into “biomimetic” nanolipoprotein particles (cNLPs) consisting of a phospholipid bilayer surrounded by apolipoprotein A1 and amphipathic polymer scaffolding moieties. Our cNLP formulation improves the water solubility of curcumin over 30-fold and produces nanoparticles with ~350 µg/mL total loading capacity for downstream in vitro and in vivo applications. We found that cNLPs were well tolerated in AG05965/MRC-5 human primary lung fibroblasts compared to cultures treated with curcumin solubilized in DMSO (curDMSO). Pre-treatment with cNLPs of quiescent G0/G1-phase MRC-5 cultures improved cell survival following 137Cs gamma ray irradiations, although this finding was reversed in asynchronously cycling log-phase cell cultures. These findings may be useful for establishing cNLPs as a method to improve curcumin bioavailability for administration as a radioprotective and/or radiomitigative agent against ionizing radiation (IR) exposures in non-cycling cells or as a radiosensitizing agent for actively dividing cell populations, such as tumors.

show abstract

Section: Resultssupporting

confidence: 80%

Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner

et al. 2022

View full text Add to dashboard Cite

show abstract

“…They are an eco-friendly and biocompatible class of amphiphilic biosurfactants that are used in a wide spectrum of medicinal application ranging from biosurfactant, cosmetic and detergent development [ 3 ]. Over the last years biosurfactants in general and SLs specifically have been used in biomedical sciences for their antifungal [ 4 ], antibacterial [ 5 , 6 ] anti-HIV [ 7 ], and anticancer [ 8 , 9 ] properties. The advantage of using SLs against cancer cells lines is their low side effect on normal tissue [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Direct Observation of Sophorolipid Micelle Docking in Model Membranes and Cells by Single Particle Studies Reveals Optimal Fusion Conditions

2020

View full text Add to dashboard Cite

Sophorolipids (SLs) are naturally produced glycolipids that acts as drug delivery for a spectrum of biomedical applications, including as an antibacterial antifungal and anticancer agent, where they induce apoptosis selectively in cancerous cells. Despite their utility, the mechanisms underlying their membrane interactions, and consequently cell entry, remains unknown. Here, we combined a single liposome assay to observe directly and quantify the kinetics of interaction of SL micelles with model membrane systems, and single particle studies on live cells to record their interaction with cell membranes and their cytotoxicity. Our single particle readouts revealed several repetitive docking events on individual liposomes and quantified how pH and membrane charges, which are known to vary in cancer cells, affect the docking of SL micelles on model membranes. Docking of sophorolipids micelles was found to be optimal at pH 6.5 and for membranes with -5% negatively charge lipids. Single particle studies on mammalian cells reveled a two-fold increased interaction on Hela cells as compared to HEK-293 cells. This is in line with our cell viability readouts recording an approximate two-fold increased cytotoxicity by SLs interactions for Hela cells as compared to HEK-293 cells. The combined in vitro and cell assays thus support the increased cytotoxicity of SLs on cancer cells to originate from optimal charge and pH interactions between membranes and SL assemblies. We anticipate studies combining quantitative single particle studies on model membranes and live cell may reveal hitherto unknown molecular insights on the interactions of sophorolipid and additional nanocarriers mechanism.

show abstract

“…However, they found that sophorolipids do not show any antibacterial action on PAO1 but only disperse biofilm formation of the bacterial strain [ 110 ]. Vasudevan and Prabhune [ 111 ] evaluated that curcumin-sophorolipid nanoparticles exhibited good antibiofilm activities by quorum quenching against P. aeruginosa [ 111 ].…”

Section: Applications Of Sophorolipids In Food and Healthmentioning

confidence: 99%

Sophorolipids—Bio-Based Antimicrobial Formulating Agents for Applications in Food and Health

Cho

Yap

et al. 2022

Molecules

View full text Add to dashboard Cite

Sophorolipids are well-known glycolipid biosurfactants, produced mainly by non-pathogenic yeast species such as Candida bombicola with high yield. Its unique environmental compatibility and high biodegradable properties have made them a focus in the present review for their promising applications in diverse areas. This study aims to examine current research trends of sophorolipids and evaluate their applications in food and health. A literature search was conducted using different research databases including PubMed, ScienceDirect, EBSCOhost, and Wiley Online Library to identify studies on the fundamental mechanisms of sophorolipids and their applications in food and health. Studies have shown that various structural forms of sophorolipids exhibit different biological and physicochemical properties. Sophorolipids represent one of the most attractive biosurfactants in the industry due to their antimicrobial action against both Gram-positive and Gram-negative microorganisms for applications in food and health sectors. In this review, we have provided an overview on the fundamental properties of sophorolipids and detailed analysis of their applications in diverse areas such as food, agriculture, pharmaceutical, cosmetic, anticancer, and antimicrobial activities.

show abstract

Photophysical studies on curcumin-sophorolipid nanostructures: applications in quorum quenching and imaging

Cited by 29 publications

References 67 publications

Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner

Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner

Direct Observation of Sophorolipid Micelle Docking in Model Membranes and Cells by Single Particle Studies Reveals Optimal Fusion Conditions

Sophorolipids—Bio-Based Antimicrobial Formulating Agents for Applications in Food and Health

Contact Info

Product

Resources

About