Photophysical properties of Radachlorin photosensitizer in solutions of different pH, viscosity and polarity

Belashov, A.V.; Zhikhoreva, A.A.; Gorbunova, I.A.; Sasin, M.E.; Shayakhmedov, Sh.S.; Semenova, I.V.

doi:10.1016/j.saa.2023.123480

Cited by 4 publications

(2 citation statements)

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“…Different aspects of variations in the photophysical properties of Radachlorin with microenvironment pH, polarity, and viscosity were intensively studied in solutions. These properties included absorption and fluorescence spectra, fluorescence quantum yield and lifetimes, fluorescence anisotropy, and rotational diffusion time (see [44] and references therein) as well as quantum yield and characteristic times of singlet oxygen phosphorescence [16]. However, some subtle processes of its performance on the cellular level are still unknown.…”

Section: Radachlorin Photosensitizermentioning

confidence: 99%

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PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro

Belashov,

Zhikhoreva,

Salova

et al. 2023

Photonics

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Variations in the fluorescence lifetimes of Radachlorin photosensitizers in HeLa and A549 cells, caused by photodynamic treatment, were studied using fluorescence lifetime imaging microscopy (FLIM). An analysis of FLIM images of the cells demonstrated a substantial decrease in the mean Radachlorin fluorescence lifetime and intensity as a result of UV irradiation of the photosensitized cells at different doses, with higher doses causing a more pronounced decrease in the mean fluorescence lifetime in cells. The post-treatment decrease in Radachlorin fluorescence intensity was accompanied by the appearance of an additional rapidly decaying fluorescence component and a nonlinear decrease in the weighted fluorescence lifetime obtained from double-exponential fits of time-resolved fluorescence signals. Experiments performed in the aqueous solutions of the photosensitizer revealed similar irreversible changes in the Radachlorin fluorescence lifetime and intensity. Therefore, the observed phenomena occurred most likely due to the photodegradation of the photosensitizer molecules and can be applied for dosimetry and monitoring of irradiation doses in different areas of malignant tissues in the course of photodynamic treatment.

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Section: Radachlorin Photosensitizermentioning

confidence: 99%

“…We have recently shown that time-resolved fluorescence spectroscopy using Radachlorin allows assessing variations in microenvironmental acidity, polarity, and viscosity [24,36,44]. The FLIM-assisted imaging provided data on the PS uptake, concentration, and fluorescence quantum yield in cells and tissues [24].…”

Section: Introductionmentioning

confidence: 99%