Photophysical properties of flavonoids extracted from Syngonanthus nitens, the golden grass

Berlim, Leonardo S.; Bezerra, Arandi Ginane; Pazin, Wallance Moreira; Ramin, Thiago Siqueira; Schreiner, W. H.; Ito, Amando Siuiti

doi:10.1016/j.jlumin.2017.10.040

Cited by 15 publications

(9 citation statements)

References 25 publications

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“…The biological characteristics above described motivated us to investigate the molecular properties of Artepillin C on aqueous solution, since most of biological molecules are embedded in fluids, especially water. This study was performed by optical absorption, steady state and time-resolved fluorescence spectroscopies, which are powerful and sensitive techniques to evaluate the protonation state, the aggregation state and the hydrophobic-hydrophilic characteristics of many natural compounds [18].…”

Section: Introductionmentioning

confidence: 99%

Optical absorption and fluorescence spectroscopy studies of Artepillin C, the major component of green propolis

Camuri

Costa

Ito

et al. 2018

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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The bioactivity of propolis against several pathogens is well established, leading to the extensive consumption of that bee product to prevent diseases. Brazilian green propolis, collected by the species Apis mellifera, is one of the most consumed in the world. The chemical composition of green propolis is complex and it has been shown that it displays antioxidant, antimicrobial, anti-inflammatory and antitumor activities, especially due to the high content of Artepillin C. The molecule is a derivative of cinnamic acid with two prenylated groups, responsible for the improvement of the affinity of the compound for lipophilic environment. A carboxylic group (COOH) is also present in the molecule, making it a pH-sensitive compound and the pH-dependent structure of Artepillin C, may modulate its biological activity related to interactions with the cellular membrane of organisms and tissues. Molecular properties of Artepillin C on aqueous solution were examined by optical absorption, steady state and time-resolved fluorescence spectroscopies. Acid-base titration based on the spectral position of the near UV absorption band, resulted in the pKa value of 4.65 for the carboxylic group in Artepillin C. In acidic pH, below the pKa value, an absorption band raised around 350nm at Artepillin C concentration above 50μM, due to aggregation of the molecule. In neutral pH, with excitation at 310nm, Artepillin C presents dual emission at 400 and 450nm. In pH close to the pKa, the optical spectra show contribution from both protonated and deprotonated species. A three-exponential function was necessary to fit the intensity decays at the different pHs, dominated by a very short lifetime component, around 0.060ns. The fast decay resulted in emission before fluorescence depolarization, and in values of fluorescence anisotropy higher than could be expected for monomeric forms of the compound. The results give fundamental knowledge about the protonation-deprotonation state of the molecule, that may be relevant in processes mediated by biological membranes.

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Section: Introductionmentioning

confidence: 99%

Optical absorption and fluorescence spectroscopy studies of Artepillin C, the major component of green propolis

Camuri

Costa

Ito

et al. 2018

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…The previous study showed that ultrasound irradiation time can be optimized at duration less than 35 minutes and the compound can be degraded at longer treatment as describe by Lui et al (2013) [17]. A study by Leonardo et al (2018) analyzes that the different yield in an antioxidant compound can be influenced by many factors such as the method of treatment, type of solvent use, temperature, pressure, natural material and method of analyzing [18].…”

Section: B Response Surface Analysis Of Bcbmentioning

confidence: 99%

Central Composite Design as a Tools for Optimization of Antioxidant Activity on Cocoa Shell Extract

Yusof¹,

Gani²,

Zaidan³

et al. 2019

IJEAT

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This study seeks to analyze and optimize the antioxidant activity thru beta carotene bleaching assay influenced by different extraction condition on extracts obtained from Malaysia Cocoa Shell. The condition was optimized by response surface methodology with five levels of the model. There are three variables involved in this study, namely: ethanol concentration (70-90 v/v %), temperature (45-65 ºC), and ultrasound irradiation time (30-60 min). The optimum condition obtains for temperature, duration of extraction and solvent concentration were 55 ºC, 45 minutes and 63.18% respectively at a constant frequency of 40 kHz. In this condition, the antioxidant activity experimental was 98.91±0.5% and predicted 98.44%. The ANOVA shows the coefficient of determination (R2 ) and the lack of fit test was 0.9846 and 0.6105 respectively. The desirability function from T-test data, modeled equation fits the data, reveals that the design can be used to predicting future observation within the design range.

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“…Nowadays, the extract of S. nitens stems has been studied as a natural product against different Candida species and clinical isolates from patients with vulvovaginal candidiasis (VVC), which results demonstrated that the antifungal properties are effective and it has the ability to inhibit the fungal‐host invasion on human cells (de Freitas Araújo et al, ; dos Santos Ramos et al, ). Recently, a work suggested that the study of crude extracts can provide explanations about the compounds behaviors and it shows the importance of possible bioinspired applications of a complete scientific understanding of S. nitens (Berlim et al, ). Despite the considerable amount of flavonoids in the composition of GG, low attention has been paid to their possible antioxidant properties.…”

Section: Introductionmentioning

confidence: 99%

In vitro antioxidant properties of golden grass (Syngonanthus nitens) by electron paramagnetic resonance

Barroso

Berlim

Ito

et al. 2019

Food Science & Nutrition

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The in vitro antioxidant properties of golden grass (GG), a grass‐like herb ( Syngonanthus nitens ), were investigated by electron paramagnetic resonance (EPR) spectroscopy. We measured the antioxidant capacity of methanolic extracts based on their ability to scavenge 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical. The kinetics of reaction between DPPH and GG extract was determined. This kinetics followed a biexponential decay, and this behavior was attributed to different flavonoids acting together as antioxidants. Isoorientin and luteolin, which are two of the eight flavonoids found in GG extract, were used to investigate kinetics of reaction between DPPH and both the flavonoids acting separately and together. The antioxidant activity of GG extract was determined in terms of the vitamin C equivalent antioxidant capacity (VCEAC). Compared to other well‐known plant‐based antioxidants, such as pulp and peels of fruit and vegetables, S. nitens presented a high antioxidant capacity (VCEAC = 1,485 ± 198 mg/100 g), indicating that it should be regarded as a valuable source of antioxidants and also that it may bestow health benefits when consumed.

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Photophysical properties of flavonoids extracted from Syngonanthus nitens, the golden grass

Cited by 15 publications

References 25 publications

Optical absorption and fluorescence spectroscopy studies of Artepillin C, the major component of green propolis

Optical absorption and fluorescence spectroscopy studies of Artepillin C, the major component of green propolis

Central Composite Design as a Tools for Optimization of Antioxidant Activity on Cocoa Shell Extract

In vitro antioxidant properties of golden grass (Syngonanthus nitens) by electron paramagnetic resonance

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