Photophysical characterization and BSA interaction of the direct ring carboxy functionalized unsymmetrical NIR cyanine dyes

Saikiran, Maryala; Sato, Daisuke; Pandey, Shyam S.; Ohta, Takeshi; Hayase, Shuzi; Kato, Tamaki

doi:10.1016/j.dyepig.2017.01.015

Cited by 22 publications

(10 citation statements)

References 39 publications

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“…Therefore, on the graph in Figure 5 A, which characterizes the total fluorescent signal (total radiant efficiency) for all the dissected organs, there is a tendency to increase regardless of the administered object (free or encapsulated fluorescent conjugate), which is similar to the results described in [ 74 ]. A significant change in intensity may be associated with the active interaction of the Cy7 dye with biomolecules, such as proteins, since it is known from a number of works that the higher the protein concentration, the higher the fluorescence intensity of cyanine dyes is [ 75 , 76 , 77 ]. Thus, for the case of capsule administration, this indirectly indicates their rapid degradation and release of the BSA-Cy7 conjugate encapsulated in the inner space.…”

Section: Resultsmentioning

confidence: 99%

Renal Artery Catheterization for Microcapsules’ Targeted Delivery to the Mouse Kidney

et al. 2022

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The problem of reducing the side effects associated with drug distribution throughout the body in the treatment of various kidney diseases can be solved by effective targeted drug delivery. The method described herein involves injection of a drug encapsulated in polyelectrolyte capsules to achieve prolonged local release and long-term capillary retention of several hours while these capsules are administered via the renal artery. The proposed method does not imply disruption (puncture) of the renal artery or aorta and is suitable for long-term chronic experiments on mice. In this study, we compared how capsule size and dosage affect the target kidney blood flow. It has been established that an increase in the diameter of microcapsules by 29% (from 3.1 to 4.0 μm) requires a decrease in their concentration by at least 50% with the same suspension volume. The photoacoustic method, along with laser speckle contrast imaging, was shown to be useful for monitoring blood flow and selecting a safe dose. Capsules contribute to a longer retention of a macromolecular substance in the target kidney compared to its free form due to mechanical retention in capillaries and slow impregnation into surrounding tissues during the first 1–3 h, which was shown by fluorescence tomography and microscopy. At the same time, the ability of capillaries to perform almost complete “self-cleaning” from capsular shells during the first 12 h leads to the preservation of organ tissues in a normal state. The proposed strategy, which combines endovascular surgery and the injection of polymer microcapsules containing the active substance, can be successfully used to treat a wide range of nephropathies.

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Section: Resultsmentioning

confidence: 99%

Renal Artery Catheterization for Microcapsules’ Targeted Delivery to the Mouse Kidney

et al. 2022

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“…On the other hand, the degradation of top layers of particle shells in the alveoli space would result in the release of Cy7 into the lung fluid, which in turn will affect the fluorescence intensity. For example, a number of works have shown the rise of fluorescence upon degradation of carriers in vivo . , Cyanine dyes tend to increase their quantum yield with increasing protein concentration in the solution . Therefore, over time, it does not seem correct to directly correlate the fluorescence intensity and the number of carriers with fluorescence.…”

Section: Resultsmentioning

confidence: 99%

Barnase-Loaded Vaterite Nanoparticles Functionalized by EpCAM Targeting Vectors for the Treatment of Lung Diseases

Abakumova

Gusliakova

Cvjetinovic

et al. 2022

ACS Appl. Nano Mater.

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Protein-based therapeutics is an emerging technology to combat various pathologies, including lung diseases. Particle-mediated delivery promotes its accumulation and increases therapeutic efficacy, minimizing side effects. Barnase (Bn) is a highly stable bacterial protein with ribonuclease (RNase) activity that affects cell proliferation. Encapsulation of Bn in particulate forms like liposomes or inorganic nanoparticles leads to its prolonged circulation in blood, but effective accumulation in targeted sites and subsequent internalization are still limited. In this work, we focus on the development of an effective delivery system with ribonuclease activity and specific accumulation in lung tissues. We fabricate multilayer Bn-loaded vaterite nanoparticles conjugated with targeting vector–aptamer specific to the epithelial cell adhesion molecule (EpCAM). Although two Bn layers in the particles do not result in an increase in loading efficiency, they do contribute significantly to ribonuclease activity (44.9 nM/min for (DS/Bn)2/DS vs 14.8 nM/min for DS/Bn/Ds) and enzyme stability (up to 90% of Bn activity for 7 days). We show that the conjugation of particles with EpCAM aptamer drastically increases particle internalization in human lung adenocarcinoma cells with a significant cytotoxic effect. In vivo biodistribution analysis reveals that intratracheal instillation of EpCAM-modified (DS/Bn)2/DS particles causes better accumulation of particles in lung tissues as identified by fluorescence imaging tomography and confocal microscopy. Thus, the developed targeted bilayer vaterite nanoparticles can be a promising protein-based delivery system for the treatment of lung diseases.

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“…Recent studies have shown the importance of the indole nucleus with respect to its anticancer activities (Singh et al, 2010;Shaveta, 2014) and in its biological applications as biosensors (Saikiran et al, 2017;Guo et al, 2015). As part of our studies of indole derivatives, we report here the crystal structure and optical properties of the title salt, C 15 H 22 N + ÁI À , bearing a quaternized indolic N atom with a butyl side chain.…”

Section: Structure Descriptionmentioning

confidence: 97%

1-Butyl-2,3,3-trimethylindol-1-ium iodide

et al. 2018

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Photophysical characterization and BSA interaction of the direct ring carboxy functionalized unsymmetrical NIR cyanine dyes

Cited by 22 publications

References 39 publications

Renal Artery Catheterization for Microcapsules’ Targeted Delivery to the Mouse Kidney

Renal Artery Catheterization for Microcapsules’ Targeted Delivery to the Mouse Kidney

Barnase-Loaded Vaterite Nanoparticles Functionalized by EpCAM Targeting Vectors for the Treatment of Lung Diseases

1-Butyl-2,3,3-trimethylindol-1-ium iodide

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