Photophosphorylation and oxidative phosphorylation in intact cells and chromatophores of an aerobic photosynthetic bacterium, Erythrobacter sp. strain OCh114

Okamura, Kazuo; Mitsumori, Fumiyuki; Ito, O.; Takamiya, Ken Ichiro; Nishimura, Mitsuo

doi:10.1128/jb.168.3.1142-1146.1986

Cited by 33 publications

(27 citation statements)

References 16 publications

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“…The importance of light controlling AAP bacterial activity has been shown by several pure culture studies (Shiba, 1984;Okamura et al, 1986;Holert et al, 2011;Tomasch et al, 2011;Hauruseu and Koblížek, 2012). However, we did not see a significant effect of light in short term incubations.…”

Section: Discussioncontrasting

confidence: 56%

“…This presumed advantage led to the hypothesis that one type of photoheterotroph, aerobic anoxygenic phototrophic (AAP) bacteria, would be most abundant in oligotrophic systems (Kolber et al, 2000), but subsequent work demonstrated that AAP bacteria are abundant in eutrophic estuaries and coastal waters when compared with the open oceans Jiao et al, 2007;Lami et al, 2007;Waidner and Kirchman, 2007;Ritchie and Johnson, 2012). Light has been shown to positively affect the growth rate and other aspects of AAP bacterial activity in laboratory experiments (Shiba, 1984;Okamura et al, 1986;Holert et al, 2011;Tomasch et al, 2011;Hauruseu and Koblížek, 2012), but it has complex effects on natural microbial communities which include photoheterotrophic microbes Straza and Kirchman, 2011;Ruiz-González et al, 2012a;Ruiz-González et al, 2012b). Data on growth-related activity in natural communities would help elucidate the advantages of photoheterotrophy and whether photoheterotrophic microbes change models of carbon cycling (Karl, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Leucine incorporation by aerobic anoxygenic phototrophic bacteria in the Delaware estuary

2014

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Aerobic anoxygenic phototrophic (AAP) bacteria are well known to be abundant in estuaries, coastal regions and in the open ocean, but little is known about their activity in any aquatic ecosystem. To explore the activity of AAP bacteria in the Delaware estuary and coastal waters, single-cell 3 H-leucine incorporation by these bacteria was examined with a new approach that combines infrared epifluorescence microscopy and microautoradiography. The approach was used on samples from the Delaware coast from August through December and on transects through the Delaware estuary in August and November 2011. The percent of active AAP bacteria was up to twofold higher than the percentage of active cells in the rest of the bacterial community in the estuary. Likewise, the silver grain area around active AAP bacteria in microautoradiography preparations was larger than the area around cells in the rest of the bacterial community, indicating higher rates of leucine consumption by AAP bacteria. The cell size of AAP bacteria was 50% bigger than the size of other bacteria, about the same difference on average as measured for activity. The abundance of AAP bacteria was negatively correlated and their activity positively correlated with light availability in the water column, although light did not affect 3 H-leucine incorporation in light-dark experiments. Our results suggest that AAP bacteria are bigger and more active than other bacteria, and likely contribute more to organic carbon fluxes than indicated by their abundance.

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Leucine incorporation by aerobic anoxygenic phototrophic bacteria in the Delaware estuary

2014

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“…The photosynthetic competence of the AAPs has been demonstrated by spectroscopic analyses (9,27,32) and infrared (IR) kinetic fluorescence measurements (16,21). It was shown that light exposure of AAP cells inhibits respiration (10,16) and increases their cellular ATP concentration (2,25). This indicates that AAPs are able to partially replace oxidative phosphorylation with photophosphorylation.…”

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confidence: 99%

Influence of Light on Carbon Utilization in Aerobic Anoxygenic Phototrophs

Hauruseu

Koblížek

2012

Appl Environ Microbiol

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bAerobic anoxygenic phototrophs contain photosynthetic reaction centers composed of bacteriochlorophyll. These organisms are photoheterotrophs, as they require organic carbon substrates for their growth whereas light-derived energy has only an auxiliary function. To establish the contribution of light energy to their metabolism, we grew the phototrophic strain Erythrobacter sp. NAP1 in a carbon-limited chemostat regimen on defined carbon sources (glutamate, pyruvate, acetate, and glucose) under conditions of different light intensities. When grown in a light-dark cycle, these bacteria accumulated 25% to 110% more biomass in terms of carbon than cultures grown in the dark. Cultures grown on glutamate accumulated the most biomass at moderate light intensities of 50 to 150 mol m ؊2 s ؊1 but were inhibited at higher light intensities. In the case of pyruvate, we did not find any inhibition of growth by high irradiance. The extent of anaplerotic carbon fixation was detemined by radioactive bicarbonate incorporation assays. While the carboxylation activity provided 4% to 11% of the cellular carbon in the pyruvate-grown culture, in the glutamate-grown cells it provided only approximately 1% of the carbon. Additionally, we tested the effect of light on respiration and photosynthetic electron flow. With increasing light intensity, respiration decreased to approximately 25% of its dark value and was replaced by photophosphorylation. The additional energy from light allows the aerobic anoxygenic phototrophs to accumulate the supplied organic carbon which would otherwise be respired. The higher efficiency of organic carbon utilization may provide an important competitive advantage during growth under carbon-limited conditions.

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“…This type of photochemistry is present not only in all purple photosynthetic bacteria and Chloroflexus species but also in the aerobic, BChl-containing bacteria (27,61). However, the primary photochemistry in the RC of R. denitrificans and the light-induced pH change of the suspension disappear under anoxic conditions (27,44,45). Light stimulates growth for a short period under anoxic conditions in the presence of high concentrations of trimethylamine N-oxide or nitrate as an external electron acceptor when the cells have been precultivated aerobically in the dark (24)(25)(26).…”

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confidence: 99%

Structure of the puf operon of the obligately aerobic, bacteriochlorophyll alpha-containing bacterium Roseobacter denitrificans OCh114 and its expression in a Rhodobacter capsulatus puf puc deletion mutant

et al. 1997

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Roseobacter denitrificans (Erythrobacter species strain OCh114) synthesizes bacteriochlorophyll a (BChl) and the photosynthetic apparatus only in the presence of oxygen and is unable to carry out primary photosynthetic reactions and to grow photosynthetically under anoxic conditions. The puf operon of R. denitrificans has the same five genes in the same order as in many photosynthetic bacteria, i.e., pufBALMC. PufC, the tetraheme subunit of the reaction center (RC), consists of 352 amino acids (M r , 39,043); 20 and 34% of the total amino acids are identical to those of PufC of Chloroflexus aurantiacus and Rubrivivax gelatinosus, respectively. The N-terminal hydrophobic domain is probably responsible for anchoring the subunit in the membrane. Four heme-binding domains are homologous to those of PufC in several purple bacteria. Sequences similar to pufQ and pufX of Rhodobacter capsulatus were not detected on the chromosome of R. denitrificans. The puf operon of R. denitrificans was expressed in trans in Escherichia coli, and all gene products were synthesized. The Roseobacter puf operon was also expressed in R. capsulatus CK11, a puf puc double-deletion mutant. For the first time, an RC/light-harvesting complex I core complex was heterologously synthesized. The strongest expression of the R. denitrificans puf operon was observed under the control of the R. capsulatus puf promoter, in the presence of pufQ and pufX and in the absence of pufC. Charge recombination between the primary donor P ؉ and the primary ubiquinone Q A ؊ was observed in the transconjugant, showing that the M and L subunits of the RC were correctly assembled. The transconjugants did not grow photosynthetically under anoxic conditions.

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Photophosphorylation and oxidative phosphorylation in intact cells and chromatophores of an aerobic photosynthetic bacterium, Erythrobacter sp. strain OCh114

Cited by 33 publications

References 16 publications

Leucine incorporation by aerobic anoxygenic phototrophic bacteria in the Delaware estuary

Leucine incorporation by aerobic anoxygenic phototrophic bacteria in the Delaware estuary

Influence of Light on Carbon Utilization in Aerobic Anoxygenic Phototrophs

Structure of the puf operon of the obligately aerobic, bacteriochlorophyll alpha-containing bacterium Roseobacter denitrificans OCh114 and its expression in a Rhodobacter capsulatus puf puc deletion mutant

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