Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus

Mukherjee, Arun; Haldar, Chandana

doi:10.1016/j.jphotobiol.2014.08.022

Cited by 33 publications

(19 citation statements)

References 40 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of StAR was reported to be regulated by GATA-4 (Martin et al, 2012), steroidogenic factor-1 (SF-1) (Hoivik et al, 2010), and CCAAT/ enhancer binding protein-beta (C/EBP-beta) (Silverman et al, 2006). The inhibitory effect of melatonin on testosterone production through inhibition of various proteins and enzymes (including StAR, P450SCC and 3β-HSD) has previously been reported for animal models and cultured cells (Frungieri et al, 2005;Mukherjee and Haldar, 2014). It is still unknown, however, whether the inhibition of testosterone synthesis by melatonin is enforced through a cAMP/GATA-4 mediated pathway.…”

Section: Introductionmentioning

confidence: 94%

Inhibitory effect of melatonin on testosterone synthesis is mediated via GATA-4/SF-1 transcription factors

Qin

Zhang

Liu

et al. 2015

Reproductive BioMedicine Online

View full text Add to dashboard Cite

The aim of the present study was to elucidate whether the GATA-4/SF-1 signalling pathway is involved in the inhibitory effects of melatonin on testosterone production in both the TM3 Leydig cell line and in C57BL/6J mice. In-vitro experiments demonstrated that melatonin treatment significantly reduced testosterone levels in cell culture medium (P < 0.05 or P < 0.01); and decreased intracellular cyclic adenosine monophospha accumulation (P < 0.05 or P < 0.01) and mRNA/protein expression of GATA-4, SF-1 (NR5A1), StAR, P450SCC (CYP11A1) and 3β-HSD (P < 0.05 or P < 0.01). These effects were blocked by N-acetyl-2-benzyltryptamin, a melatonin receptor antagonist. Similar effects of melatonin on testosterone production (P < 0.05 or P < 0.01) and down-regulation of transcription factors GATA-4 and SF-1 (P < 0.01) were also observed in mice treated with intratesticular injections of melatonin. Overall, the data suggest that the inhibitory effects of melatonin on testosterone production are mediated via down-regulation of GATA-4 and SF-1 expression.

show abstract

Section: Introductionmentioning

confidence: 94%

Inhibitory effect of melatonin on testosterone synthesis is mediated via GATA-4/SF-1 transcription factors

Qin

Zhang

Liu

et al. 2015

Reproductive BioMedicine Online

View full text Add to dashboard Cite

show abstract

“…As mentioned, some parameters in the upstream HPG-axis of male Brandt's vole did not undergo a noticeable response to the 6-MBOA treatment except the KiSS-1 expression in the ARC and circulating gonadotropins declined only in the high dose group. In contrast, the expressions of StAR and CYP11a1, which encode for important enzymes involved in the synthesis of testosterone in the testis (Mukherjee and Haldar, 2014), were reduced sharply in response to 6-MBOA treatment. Further, the expressions of StAR and CYP11a1 changed more dramatically than other parameters measured previously after 6-MBOA treatment (Dai et al, 2016).…”

Section: Discussionmentioning

confidence: 88%

“…Since melatonin receptors are located in pars tuberalis of anterior pituitary (Henningsen et al, 2016;Dardente et al, 2016) and testis (Izzo et al, 2010;Mukherjee and Haldar, 2014), we propose that 6-MBOA may act as a partial melatonin agonist that could directly act on both pituitary and testis. Therefore, steroidogenic genes (StAR and CYP11a1) were directly regulated by both upstream HPG-axis and 6-MBOA, which would amplify the negative effect on testis and inhibit steroidogenic genes resulting in the decline of circulating testosterone and relative testis weight.…”

Section: Discussionmentioning

confidence: 99%

Effect of photoperiod and 6-methoxybenzoxazolinone (6-MBOA) on the reproduction of male Brandt’s voles (Lasiopodomys brandtii)

Dai

Shi

Han

et al. 2017

General and Comparative Endocrinology

View full text Add to dashboard Cite

Plant secondary metabolite 6-methoxybenzoxazolinone (6-MBOA) has been suggested to stimulate animal reproduction. 6-MBOA is detected in Leymus chinensis, a main diet of Brandt's vole (Lasiopodomys brandtii). We have previously reported a stimulatory effect of 6-MBOA on reproduction of male Brandt's voles under a short-day photoperiod. The goal of this study was to investigate the effect of 6-MBOA on reproductive physiology of male Brandt's voles under a long-day photoperiod and examine if 6-MBOA under this photoperiodic regime altered the reproductive status of male Brandt's voles differently than the short-day photoperiod. Under the long-day photoperiod, a high dose of 6-MBOA decreased KiSS-1 mRNA in the arcuate nucleus (ARC), and we also saw a decrease in circulating levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone (T). Steroidogenic acute regulatory protein (StAR) and cytochrome P450 (CYP11a1) in the testes, and relative testis weight also decreased with 6-MBOA administration. Compared to the short-day photoperiod, animals under the long-day photoperiod exhibited increased body weight as well as all other reproductive parameters. Our results showed that 6-MBOA inhibited the reproduction of male Brandt's vole under a long-day photoperiod, a stark contrast from its stimulatory effects under a short-day photoperiod. The paradoxical effects of 6-MBOA suggest it may act as a partial agonist of melatonin. These results provide insight into the complex interactions between environmental factors such as photoperiod and diet in the control of Brandt's vole reproduction.

show abstract

“…This reduction of testosterone production by melatonin (10 pM to 1 μ M) likely takes account through the binding of the pineal hormone to the MT-1 receptor expressed in Leydig cells, resulting in decreased levels of cAMP [ 137 ]. In hamsters, melatonin (10 pM to 1 μ M) suppresses testicular steroidogenesis through inhibition of several enzymes, such as steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (cytochrome P450SCC), and 3 β -hydroxysteroid dehydrogenase (3 β -HSD) [ 137 , 138 ]. In Leydig cell lines from mice, the reduction of testosterone production by melatonin (range from 1 pM to 1 μ M) is related to a decrease in cAMP levels and downregulation of GATA-4 and SF-1 transcription factors [ 139 ].…”

Section: Melatonin Influences Breast Prostate and Ovary Cancer Amentioning

confidence: 99%

Melatonin: An Anti-Tumor Agent in Hormone-Dependent Cancers

Menéndez-Menéndez

Martínez‐Campa

2018

International Journal of Endocrinology

View full text Add to dashboard Cite

Melatonin (N-acetyl-5-methoxytryptamine) is a hormone synthesized and secreted by the pineal gland mainly during the night, since light exposure suppresses its production. Initially, an implication of this indoleamine in malignant disease was described in endocrine-responsive breast cancer. Data from several clinical trials and multiple experimental studies performed both in vivo and in vitro have documented that the pineal hormone inhibits endocrine-dependent mammary tumors by interfering with the estrogen signaling-mediated transcription, therefore behaving as a selective estrogen receptor modulator (SERM). Additionally, melatonin regulates the production of estradiol through the control of the enzymes involved in its synthesis, acting as a selective estrogen enzyme modulator (SEEM). Many more mechanisms have been proposed during the past few years, including signaling triggered after activation of the membrane melatonin receptors MT-1 and MT-2, or else intracellular actions targeting molecules such as calmodulin, or binding intranuclear receptors. Similar results have been obtained in prostate (regulation of enzymes involved in androgen synthesis and modulation of androgen receptor levels and activity) and ovary cancer. Thus, tumor metabolism, gene expression, or epigenetic modifications are modulated, cell growth is impaired and angiogenesis and metastasis are inhibited. In the last decade, many more reports have demonstrated that melatonin is a promising adjuvant molecule with many potential beneficial consequences when included in chemotherapy or radiotherapy protocols designed to treat endocrine-responsive tumors. Therefore, in this state-of-the-art review, we aim to compile the knowledge about the oncostatic actions of the indoleamine in hormone-dependent tumors, and the latest findings concerning melatonin actions when administered in combination with radio- or chemotherapy in breast, prostate, and ovary cancers. As melatonin has no toxicity, it may be well deserve to be considered as an endogenously generated agent helpful in cancer prevention and treatment.

show abstract

Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus

Cited by 33 publications

References 40 publications

Inhibitory effect of melatonin on testosterone synthesis is mediated via GATA-4/SF-1 transcription factors

Inhibitory effect of melatonin on testosterone synthesis is mediated via GATA-4/SF-1 transcription factors

Effect of photoperiod and 6-methoxybenzoxazolinone (6-MBOA) on the reproduction of male Brandt’s voles (Lasiopodomys brandtii)

Melatonin: An Anti-Tumor Agent in Hormone-Dependent Cancers

Contact Info

Product

Resources

About