Photoperiod and ethylene-dependent expression of gibberellin biosynthesis gene InEKO1 during flower induction of Ipomoea nil

Marciniak, Katarzyna; Wilmowicz, Emilia; Kućko, Agata; Kopcewicz, Jan

doi:10.1007/s10535-017-0743-6

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Gibberellins are phytohormones that are mainly studied regarding their participation in the process of flowering induction in plants with various photoperiodic sensitivities [13,28,29,30]. Although this research has been carried out in a limited number of species, it seems that GAs are not a universal flowering stimulus, and it is assumed that they act in a species-specific manner.…”

Section: Gibberellin-mediated Stamen Development and Functioningmentioning

confidence: 99%

Comprehensive Insight into Gibberellin- and Jasmonate-Mediated Stamen Development

Marciniak

Przedniczek

2019

Genes

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In flowering plants, proper development of male generative organs is required for successful sexual reproduction. Stamen primordia arise in the third whorl of floral organs and subsequently differentiate into filaments and anthers. The early phase of stamen development, in which meiosis occurs, is followed by a late developmental phase, which consists of filament elongation coordinated with pollen maturation, anther dehiscence and finally viable pollen grain release. Stamen development and function are modulated by phytohormones, with a key role of gibberellins (GAs) and jasmonates (JAs). Long-term, extensive investigations, mainly involving GA/JA-deficient and GA/JA-response mutants, have led to a better understanding of the hormone-dependent molecular mechanisms of stamen development. In several species, the principal functions of GAs are to stimulate filament elongation through increased cell elongation and to promote anther locule opening. In the GA-dependent regulation of early stamen development, both the tapetum and developing pollen were identified as major targets. JAs mainly control the late stages of stamen development, such as filament elongation, viable pollen formation and anther dehiscence. A hierarchical relationship between GAs and JAs was recognized mainly in the control of late stamen development. By repressing DELLA proteins, GAs modulate the transcriptional activity of JA biosynthesis genes to promote JA production. A high level of JAs induces a complex of transcription factors crucial for normal stamen development.

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Section: Gibberellin-mediated Stamen Development and Functioningmentioning

confidence: 99%

Comprehensive Insight into Gibberellin- and Jasmonate-Mediated Stamen Development

Marciniak

Przedniczek

2019

Genes

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“…For each gene amplification, real-time quantitative PCR (qPCR) with specific primers (Table S1) and universal probe library (UPL) hydrolysis probes (Roche) was used. The actin gene (InACT4) was selected as a reference endogenous control for normalization purposes (Glazińska et al 2014;Marciniak et al 2017;Wilmowicz et al 2016b). qPCR with mixtures containing 0.1 µg of cDNA, 0.2 µM FP/RP, 0.05 µM UPL and 1 × LightCycler TaqMan Master Mix (LightCycler TaqMan Master Kit, Roche), was performed in 20 µl volumes in glass capillaries using a LightCycler 2.0 system (Roche).…”

Section: Expression Analysismentioning

confidence: 99%