Photopatterned Polydiacetylene Images Using a DNA Bio-Photomask

Abstract

We describe a novel strategy to fabricate a well-defined polydiacetylene (PDA) supramolecular pattern on polyvinylidene fluoride (PVDF) membrane utilizing a DNA bio-photomask. By focusing on that, the absorption spectrum of DNA molecules having a λmax at 260 nm overlaps with the wavelength at which the photopolymerization of the diacetylene monomer occurs, DNA molecules are used to define specific patterns on PVDF membranes coated with diacetylene lipids by shielding the applied 254 nm UV light and consequently preventing the photopolymerization of the lipids. As a result, the DNA-covered regions retain the original white color on the membrane while the regions uncovered by DNA molecules undergo the color change to blue through the photopolymerization by 254 nm UV irradiation. On the basis of the selective illumination through a DNA photomask, we precisely manufacture specific patterns using a microarray spotting method and also demonstrate the capability of this strategy to achieve a novel colorimetric DNA sensor without any complicated process.

3D Printing of Polydiacetylene Photocomposite Materials: Two Wavelengths for Two Orthogonal Chemistries

“…Meanwhile, due to the innate weak mechanical strength of conjugated PDA, the traditional method to fabricate patterned materials is mainly limited to the usage of photomasks. 19,20 Although photopolymerization possesses the advantage of excellent spatial and temporal control, until now, there are very few reports on real spatial controlled photopolymerization of PDA and the subsequent application in 3D printing. 21 One possible solution to this problem would be the construction of a composite between PDA and polyacrylates, considering the strong mechanical strength and vast application of photocurable polyacrylates in the field of 3D printing.…”

“…In addition, huge efforts were devoted to chemical synthesis and structural modification of PDA derivatives in many cases. , Therefore, it is of great significance and necessity to develop green chemistry approaches and explore new research fields for PDA, and the exploitation of existing and commercial DAs for further studies remains a very attractive alternative approach. Meanwhile, due to the innate weak mechanical strength of conjugated PDA, the traditional method to fabricate patterned materials is mainly limited to the usage of photomasks. , Although photopolymerization possesses the advantage of excellent spatial and temporal control, until now, there are very few reports on real spatial controlled photopolymerization of PDA and the subsequent application in 3D printing . One possible solution to this problem would be the construction of a composite between PDA and polyacrylates, considering the strong mechanical strength and vast application of photocurable polyacrylates in the field of 3D printing …”

Biological Lenses as a Photomask for Writing Laser Spots into Ferroelectric Crystals

“…Analogies and discrepancies between the real cells and the corresponding RI imprints have been discussed, also in relation to the anisotropy of the PR effect in x-cut LN. Differently from previous works (refs and ), the induced modification concerns the shaping of the RI in bulk LN without changing the topography of the substrate. The motivation of the paper stands in the establishment of a printing technique to fix into a solid material the optical fingerprint of living samples.…”

“…5 Instead, Jung et al demonstrated that DNA molecules behave as biophotomasks to create specific patterns on PVDF membranes. 6 PL through a living sample or biomolecules is part of a wider contest where the paradigm is the employment of the natural interactions between the living sample and light, thus integrating them as active optical elements. 7 Excellent examples are the biolasing 8 and photonic structures made of bacteria suspensions.…”

“…Kavand and co-workers demonstrated that direct-cell PL can be realized for PDMS surface modification in order to make more efficient cell-imprinting based experiments . Instead, Jung et al demonstrated that DNA molecules behave as biophotomasks to create specific patterns on PVDF membranes . PL through a living sample or biomolecules is part of a wider contest where the paradigm is the employment of the natural interactions between the living sample and light, thus integrating them as active optical elements .…”