Photopatternable materials for guided cell adhesion and growth

Kourti, Dimitra; Kanioura, Anastasia; Chatzichristidi, Margarita; Beltsios, K.; Kakabakos, Sotirios; Petrou, Panagiota

doi:10.1016/j.eurpolymj.2021.110896

Cited by 9 publications

(7 citation statements)

References 114 publications

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Section: Micro/nanomoldingmentioning

confidence: 99%

“…This technique is considered an efficient tool for the mass production of large structures (300 mm); however, it can even be used for nanostructures down to 20 nm, among which different molds have been designed depending on the size and shape of the structures. [86,87] In existing studies, microstructures have been fabricated on a uniaxially stretched polymeric anisotropic layer through hot embossing processing, which enhanced the efficiency and replication accuracy of LGPs. [88] By molding a polymer in the viscous state during hot embossing, several typical polymer structures can be realized with high aspect ratios and multiple layers.…”

Section: Nanoimprintingmentioning

confidence: 99%

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Current and Future Trends for Polymer Micro/Nanoprocessing in Industrial Applications

et al. 2022

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Polymers are widely used in optical devices, electronic devices, energy‐harvesting/storage devices, and sensors, owing to their low weight, excellent flexibility, and simple fabrication process. With advancements in micro/nanoprocessing techniques and more demanding application requirements, it is becoming necessary to realize high‐resolution fabrication of polymers to prepare miniaturized devices. This is particularly because conventional processing technologies suffer from high thermal stress and strong adhesion/friction, which can irreversibly damage the micro/nanostructures of miniaturized devices. In addition, although the use of advanced fabrication methods to prepare high‐resolution micro/nanostructures is explored, these methods are limited to laboratory research or small‐batch production. This review focuses on the micro/nanoprocessing of polymeric materials and devices with high spatial precision and replication accuracy for industrial applications. Specifically, the current state‐of‐the‐art techniques and future trends for micro/nanomolding, high‐energy beam processing, and micro/nanomachining are discussed. Moreover, an overview of the fabrication and applications of various polymer‐based elements and devices such as microlenses, biosensors, and transistors is provided. These techniques are expected to be widely applied for multiscale and multimaterial processing as well as for multifunction integration in next‐generation integrated devices, such as photoelectric, smart, and biodegradable devices.

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Section: Micro/nanomoldingmentioning

confidence: 99%

Section: Nanoimprintingmentioning

confidence: 99%

Current and Future Trends for Polymer Micro/Nanoprocessing in Industrial Applications

et al. 2022

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“…Coating properties including surface morphology, topography, and chemistry are known to significantly affect cell adhesion, orientation, guidance, differentiation, proliferation, and gene expression. [1][2][3][4] Such coatings have also found effective applications in biosensors, biochips, drug delivery devices, prostheses, and implants. A diverse set of biocompatible polymers from synthetic and natural origin may be used.…”

Section: Introductionmentioning

confidence: 99%

Atmospheric Plasma‐Assisted Deposition and Patterning of Natural Polymers

Arkhangelskiy

Quaranta

Motta

et al. 2022

Adv Materials Inter

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Plasma‐assisted deposition is a facile, yet sophisticated method to form biocompatible coatings on materials and introduce specific surface interactions. The plasma process provides unique features such as surface activation, functionalization, and assisted polymerization, all of which can be obtained under low power and room temperature conditions. Plasma‐assisted deposition can further provide coatings with enhanced adhesion and stability. Here, it is reported for the first time, a method for the controlled plasma deposition of the versatile biomaterial chitosan on a range of substrates – soda‐lime glass, metal alloy (Ti4Al6V), thermoplastic polymer (polyethylene terephthalate), and silicone rubber (poly(dimethylsiloxane)). The deposited chitosan films are characterized by atomic force microscopy, scanning electron microscopy and Fourier transform infrared spectroscopy, and evaluated for adhesion and stability. The proposed method is also successfully optimized for the deposition of multiple layers of different biomaterials. Specifically, coatings comprising alternate chitosan and silk fibroin layers are realized, together with patterned surfaces with programmable surface composition. The biological response of the chitosan‐on‐fibroin and fibroin‐on‐chitosan surfaces with and without patterning are investigated using cell culture experiments. Selective area deposition enables the development of improved surface finishes for biomedical devices.

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“…The precise and selective deposition of molecules on substrates, known as molecular printing, has developed extensively in recent years. − Modifying the chemical properties of surfaces tunes their interactions with molecules and nano- and microscale objects, , including cells. , Patterned surfaces are of particular importance in biological applications such as biosensors, ,− bioelectronics, , and cell growth assays. ,, Molecular printing also enables the generation of multiplexed arrays to explore the behavior of cells and biomolecules in a wide variety of microenvironments . Surface gradients, such as of hydrophobicity or the concentration of specific binding sites, are used to study cell adhesion and migration. , Surface gradients also allow for the transport of droplets via a ratchet mechanism , and the generation of solvent flows in microfluidic systems .…”

Section: Introductionmentioning

confidence: 99%

“…9,10 Patterned surfaces are of particular importance in biological applications such as biosensors, 3,11−15 bioelectronics, 16,17 and cell growth assays. 2,9,18 Molecular printing also enables the generation of multiplexed arrays to explore the behavior of cells and biomolecules in a wide variety of microenvironments. 6 Surface gradients, such as of hydrophobicity or the concentration of specific binding sites, are used to study cell adhesion and migration.…”

Section: Introductionmentioning

confidence: 99%

UV- and Visible-Light Photopatterning of Molecular Gradients Using the Thiol–yne Click Reaction

Mitmoen

Kedem

2022

ACS Appl. Mater. Interfaces

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The rational design of chemical coatings is used to control surface interactions with small molecules, biomolecules, nanoparticles, and liquids as well as optical and other properties. Specifically, micropatterned surface coatings have been used in a wide variety of applications, including biosensing, cell growth assays, multiplexed biomolecule interaction arrays, and responsive surfaces. Here, a maskless photopatterning process is studied, using the photocatalyzed thiol−yne "click" reaction to create both binary and gradient patterns on thiolated surfaces. Nearly defect-free patterns are produced by first coating glass surfaces with mercaptopropylsilatrane, a silanizing agent that forms smoother self-assembled monolayers than the commonly used 3-mercaptopropyltrimethoxysilane. Photopatterning is then performed using UV (365 nm) or visible (405 nm) light to graft molecules onto the surface in tunable concentrations based on the local exposure. The technique is demonstrated for multiple types of molecular grafts, including fluorescent dyes, poly(ethylene glycol), and biotin, the latter allowing subsequent deposition of biomolecules via biotin− avidin binding. Patterning is demonstrated in water and dimethylformamide, and the process is repeated to combine molecules soluble in different phases. The combination of arbitrary gradient formation, broad applicability, a low defect rate, and fast prototyping thanks to the maskless nature of the process creates a particularly powerful technique for molecular surface patterning that could be used for a wide variety of micropatterned applications.

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Photopatternable materials for guided cell adhesion and growth

Cited by 9 publications

References 114 publications

Current and Future Trends for Polymer Micro/Nanoprocessing in Industrial Applications

Current and Future Trends for Polymer Micro/Nanoprocessing in Industrial Applications

Atmospheric Plasma‐Assisted Deposition and Patterning of Natural Polymers

UV- and Visible-Light Photopatterning of Molecular Gradients Using the Thiol–yne Click Reaction

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