Photonic characteristics and ex vivo imaging of Escherichia coli-Xen14 within the bovine reproductive tract

Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays

“…The test results show that bioluminescence intensity dropped dramatically after one day without losing viability as shown in Fig. S1 in Supplemental Information, similar to a previous report in a static culture [ 59 ]. The reduction in luminescence may reflect a change in the number of metabolically active cells, which can produce a luminescent signal by the luciferin-luciferase system [ 60 ], and switching to an antibiotic environment may also induce dormancy in bacterial cells without a loss in viability since biofilm cells typically have a lower metabolism and can enter a persister cell state [ 61 ].…”

HORSE SPECIES SYMPOSIUM: A novel approach to monitoring pathogen progression during uterine and placental infection in the mare using bioluminescence imaging technology and lux-modified bacteria1,2

“…Conventional models to localize or estimate bacterial populations and invasiveness in vivo typically require extensive sampling procedures at each sampling time point and considerable time and labor to establish cultures (Curbelo et al, 2010). A more recent approach has been the use of bacterial luciferases (e.g., photonemitting indicators) to label pathogens and monitor their progression during infections in rodents (Contag ot aL, 1995).…”

“…The use of bioluminescent bacteria, such as E. coli-pAKl-lux or E. coZi!-Xenl4, coupled with bioluminescence imaging technologies, may represent an efficient model for achieving a greater understanding of the pathogenesis of uterine and placental infections in domestic species. Some studies have established positive relationships between bioluminescent signals and bacterial populations for gram-negative bacteria (r = 0.99; Kadurugamuwa et al, 2005), whereas other investigators have described and validated bioluminescent bacterial models, both in vitro (Moulton et al, 2006(Moulton et al, , 2009c and ex vivo (Moulton et al, 2009a,d;Curbelo et al, 2010). More recently, studies have reported the use of bioluminescent reporters and biophotonic imaging technology to study mechanisms of pathogenesis of such pathogens as Staphylococcus aureus (Kuklin et al, 2003), Aspergillus fmnigatus (Brock et al, 2008), Listeria monocytogenes (Konjufca and Miller, 2009), E. coli (Foucault et al, 2010), and Mycobacterium tuberculosis (Andreu et al, 2010); however, these studies have focused on the use of the mouse model.…”

“…For instance, Moulton et al (2009d) monitored in real time in situ pathogen progression of bioluminescent Salmonella in the gastrointestinal tract of swine. Studies have also evaluated fetal lambs delivered to bioluminescent E. co/z-infected ewes ex vivo (Moulton et al, 2009a,b) and detection of bioluminescent E. coli within the excised bovine reproductive tract (Curbelo et al, 2010). Others have used the same approach for noninvasive disease monitoring {Edwardsiella ictaluri) in living fish (Karsi et al, 2006).…”