Photon-counting H33D detector for biological fluorescence imaging

Michalet, Xavier; Siegmund, Oswald H. W.; Vallerga, J. V.; Jelinsky, Patrick; Millaud, J.; Weiss, Shimon

doi:10.1016/j.nima.2006.05.155

Cited by 40 publications

(25 citation statements)

References 15 publications

(21 reference statements)

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“…detecting electrons in angle-resolved photoemission spectroscopy, ARPES, on synchrotron sources [10] as well as photons in fluorescence lifetime imaging [11] requiring sub-ns temporal resolution). With the advent of neutron sensitive MCPs all the advancements in this technology can now be applied to NRAI.…”

Section: Methodsmentioning

confidence: 99%

High Resolution Neutron Resonance Absorption Imaging at a Pulsed Neutron Beamline

Tremsin¹,

McPhate²,

Vallerga³

et al. 2012

IEEE Trans. Nucl. Sci.

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The existence of resonance peaks in neutron absorption spectra in the epithermal range of energies enables unique non-destructive testing techniques. The deep penetration of epithermal neutrons provides an opportunity to perform a compositional analysis of a sample which is opaque to X-rays and thermal neutrons. The neutron resonances in the transmission spectra constitute a characteristic pattern for many isotopes, which can be used to identify the isotope and to map the distribution of the isotope in a sample. The neutron transmission spectra can be measured with the time of flight (TOF) technique using a pulsed neutron source. Combining this method with a high resolution neutron counting detector enables substantial improvements of spatial resolution of neutron resonance transmission imaging. Such a detector has been developed to register neutrons with 55 spatial and 10-1000 ns temporal resolution Our proof-of-principle experiments at the ISIS pulsed neutron spallation source demonstrate that compositional analysis of multi-element samples can now be performed with spatial resolution. Images of a test mask consisting of thick foils of Au, Ag, In and Gd were collected in the 1-100 eV energy range. The experimental results demonstrate the potential for compositional analysis via resonance absorption transmission with high spatial resolution. In-bulk temperature measurement through Doppler broadening analysis will also benefit from this technique.

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Section: Methodsmentioning

confidence: 99%

High Resolution Neutron Resonance Absorption Imaging at a Pulsed Neutron Beamline

Tremsin¹,

McPhate²,

Vallerga³

et al. 2012

IEEE Trans. Nucl. Sci.

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“…The DL detector has been applied to the investigation of time-and spectrally resolved fluorescence of photosynthetic fluorophores in the cyanobacterium A. marina (Petrášek et al 2005). Currently, two-dimensional DL detectors, with the two delay lines oriented perpendicularly to each other, are being developed (Czasch et al 2007b;Michalet et al 2006). Other promising designs include devices with image charge readout (Czasch et al 2007a) and avalanche photodiode arrays (Michalet et al 2007;Niclass et al 2008).…”

Section: Methodsmentioning

confidence: 99%

Wide-field photon counting fluorescence lifetime imaging microscopy: application to photosynthesizing systems

2009

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Fluorescence lifetime imaging microscopy (FLIM) is a technique that visualizes the excited state kinetics of fluorescence molecules with the spatial resolution of a fluorescence microscope. We present a scanningless implementation of FLIM based on a time- and spacecorrelated single photon counting (TSCSPC) method employing a position-sensitive quadrant anode detector and wide-field illumination. The standard time-correlated photon counting approach leads to picosecond temporal resolution, making it possible to resolve complex fluorescence decays. This allows parallel acquisition of time-resolved images of biological samples under minimally invasive low-excitation conditions (<10 mW/cm(2)). In this way unwanted photochemical reactions induced by high excitation intensities and distorting the decay kinetics are avoided. Comparably low excitation intensities are practically impossible to achieve with a conventional laser scanning microscope, where focusing of the excitation beam into a tight spot is required. Therefore, wide-field FLIM permits to study Photosystem II (PS II) in a way so far not possible with a laser scanning microscope. The potential of the wide-field TSCSPC method is demonstrated by presenting FLIM measurements of the fluorescence dynamics of photosynthetic systems in living cells of the chlorophyll d-containing cyanobacterium Acaryochloris marina.

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“…Different read-out architectures for photon counting imaging detectors exist, such as crossed delay line anodes, wedge and strip anodes or quadrant anodes (Michalet et al, 2007(Michalet et al, , 2013. Quadrant anode detectors (Emiliani et al, 2003;Kemnitz et al, 1997;Petrášek et al, 2009;Spitz et al, 2008;Vitali et al, 2010Vitali et al, , 2011 and crossed-delay line anode detectors (Michalet et al, 2006(Michalet et al, , 2009 for wide-field imaging with picosecond timing resolution have been developed, thus enabling wide-field TCSPC-based FLIM with picosecond time resolution. Conventional photon pile-up restrictions still applythey can typically only time a single photon per excitation cycle in the entire field of view (although advanced readout architectures allowing multi-photon hits to be detected have been designed (Jagutzki et al, 2002)) -but these devices combine single photon sensitivity with wide-field detection and picosecond timing resolution.…”

Section: Wide-field Tcspcmentioning

confidence: 99%

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

et al. 2015

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Photon-counting H33D detector for biological fluorescence imaging

Cited by 40 publications

References 15 publications

High Resolution Neutron Resonance Absorption Imaging at a Pulsed Neutron Beamline

High Resolution Neutron Resonance Absorption Imaging at a Pulsed Neutron Beamline

Wide-field photon counting fluorescence lifetime imaging microscopy: application to photosynthesizing systems

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

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