Photomodulation multiple sessions as a promising preventive therapy for medication-related osteonecrosis of the jaws after tooth extraction in rats

Abstract

The aim of this study was to evaluate the effects of photobiomodulation (PBM) with multiple sessions of low-level laser on the alveolar repair process of rats with major risk factors for medication-related osteonecrosis of the jaws (MRONJ). Senile rats received 0.45 mL of vehicle (VEH and VEH-PBM) or 0.45 mL of 100 μg/kg zoledronate (ZOL and ZOL-PBM) administrated intraperitoneally every two days during seven weeks. After three weeks of initiation of drug treatment the first lower left molar was extracted. No local treatment was performed in VEH and ZOL. VEH-PBM and ZOL-PBM were submitted to laser irradiation (660 ± 10 nm; 0.035 W; 2.1 J; 60 s) on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days after tooth extraction. Histological sections of the hemimandible were submitted to histopathological and histomorphometric analysis, as well as to histochemistry for collagen fiber maturation and immunohistochemistry for pro-inflammatory cytokines. In ZOL, general impairment of tissue repair, areas with osteonecrosis, lower newly formed bone tissue (NFBT), smaller amount of mature collagen fibers and increased immunoreactivity for TNFα, IL-1β and IL-6 were observed when compared to VEH and VEH-PBM. ZOL-PBM showed significant improvement in some parameters compared to ZOL, such as positive repair tissue, higher NFBT, greater amount of mature collagen fibers, besides TNFα and IL-1β immunoreactivity decrease. Zoledronate treatment severely compromised the tissue repair process of the tooth extraction site in rats with major risk factors for MRONJ. Based on parameters employed in the present study, PBM in multiple sessions can improve the alveolar repair process, constituting a promising preventive therapy to avoid the onset of post-extraction MRONJ.

Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats

“…The ligature was maintained for three weeks in order to induce experimental periodontitis (EP) (Fig. 6C) 67,68 .

Figure 6Experimental design. ( A ) Scheme illustrating experimental procedures performed during the study.

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The Effectiveness of the Low-Level Laser, Antibiotic and Surgical Therapy in the Treatment of Medication-Related Osteonecrosis of the Jaws: A Case Report

“…LLL therapy, which is also called photobiomodulation, has properties that could favor the process of tissue repair. 15,31 LLL therapy is able to modulate the metabolism of human osteoblasts by increasing their proliferation and differentiation. 32 Moreover, LLL therapy is claimed to have beneficial analgesic and anti-inflammatory effects.…”

“…Other reactions increase cell proliferation and cell differentiation when they occur, involving the transduction of signals to the cytoplasm level. 14 Previous animal studies by our research group 15 and other research groups [16][17][18] have shown that LLL therapy after extraction improves the alveolar repair process. Similarly, clinical studies have also indicated that LLL therapy has positive effects on the main signs and symptoms during the post-extraction wound healing process.…”

Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass‐ceramic materials

“…The negative controls consisted of specimens submitted to the procedures previously mentioned without the primary antibodies (Benetti et al 2018b, Dal-Fabbro et al 2019. With the aid of image analysis software (91000, Leica LAS 4.12; Leica Microsystems), the area corresponding to immunolabeling was delineated using the colour threshold tool to obtain the immunolabeling optical density, expressed as random unit percentage of optical density (mean AE SD) (Azuma et al 2018, Statkievicz et al 2018.…”