As part of a large investigation with Hubble Space Telescope to study the faintest stars within the globular cluster ω Centauri, in this work we present early results on the multiplicity of its main sequence (MS) stars, based on deep optical and near-infrared observations. By using appropriate color-magnitude diagrams we have identified, for the first time, the two main stellar populations I, and II along the entire MS, from the turn-off towards the hydrogen-burning limit. We have compared the observations with suitable synthetic spectra of MS stars and conclude that the two MSs are consistent with stellar populations with different metallicity, helium, and light-element abundance. Specifically