Photometric determination of human serum bromide levels—a convenient biomonitoring parameter for methyl bromide exposure

Müller, Michael; Reinhold, Peter; Lange, Martina; Zeise, Marc L.; Jürgens, Uwe; Hallier, Ernst

doi:10.1016/s0378-4274(99)00042-9

Cited by 27 publications

(18 citation statements)

References 3 publications

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“…Plasma bromide levels were determined from the EDTA blood samples using a validated method described in detail elsewhere (Mu¨ller et al 1999). The photometric method is based on the oxidation of bromide to bromine, which reacts with phenolsulfonphthalein (k max 356 nm) to yield 3',3'',5',5''-tetrabromophenolsulfonphthalein (k max 589 nm).…”

Section: Determination Of Plasma Bromide Levelsmentioning

confidence: 99%

Phenotyping of human glutathione S -transferase hGSTT1-1: a comparison of two ex vivo routine procedures

Müller

Bünger

Voss

et al. 2002

Archives of Toxicology

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The human glutathione S-transferase hGSTT1-1 is characterized by a polymorphism displaying three phenotypes: the "non-conjugator" (NC) phenotype expresses no or only a residual activity due to a homozygous deletion of the hGSTT1 gene, a medium hGSTT1-1 activity can be demonstrated for the "low conjugator" (LC) phenotype as the heterozygous bearer of one hGSTT1 allele, and a high hGSTT1-1 activity is detected for the "high conjugator" (HC) phenotype as the homozygous bearer of two hGSTT1 alleles. We have developed a routine ex vivo photometric phenotyping procedure based on the determination of bromide release rates from the hGSTT1-1-catalyzed glutathione conjugation of the substrate methyl bromide in EDTA blood samples under standard conditions (1,000 ppm methyl bromide, 10 min incubation). The bromide release rates were standardized to the hemoglobin (Hb) value. Twenty-six individuals were phenotyped following the new procedure. Four individuals were classified as NCs (24-33 pmol Br(-)/mg Hb per min), 21 individuals were regarded as LCs (107-206 pmol Br(-)/mg Hb per min) and one person of the study group was designated HC (236 pmol Br(-)/mg Hb per min). The results were validated by qualitative hGSTT1 genotyping and demonstrated a 100% match for conjugators and non-conjugators. A second HPLC phenotyping routine procedure based on the formation of S-methylglutathione from methyl chloride in erythrocyte lysate incubations (Müller et al. 2001, Arch Toxicol 74:760-767) was established and validated by genotyping. The phenotyping results obtained with both methods were correlated, resulting in a good correlation with R(2)=0.64 (y=0.8997x +51.535). Three distinct phenotype clusters for NCs, LCs and HCs, consistent with the proposed genetics, were demonstrated. Assay-dependent storage experiments revealed an excellent stability of the hGSTT1-1 activity. In conclusion, the evaluated methods provide powerful tools for determination of hGSTT1-1 activity as a clinical parameter.

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Section: Determination Of Plasma Bromide Levelsmentioning

confidence: 99%

Phenotyping of human glutathione S -transferase hGSTT1-1: a comparison of two ex vivo routine procedures

Müller

Bünger

Voss

et al. 2002

Archives of Toxicology

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show abstract

“…Numerous methods have been reported for the determination of bromide in blood and/or urine using ion chromatography [8], gas chromatography (GC) [1,2,[9][10][11][12][13], radio activation analysis [3], inductively coupled plasma mass spectrometry [14], X-ray spectrometry [15], cyclic voltammetry [16] and photometry [17].…”

Section: Introductionmentioning

confidence: 99%

Determination of bromide in whole blood and urine from humans using gas chromatography–mass spectrometry

Kage¹,

Kudo

Ikeda

et al. 2005

Journal of Chromatography B

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“…8 This case revealed not only neurological disturbances such as visual and gait disturbance, but also increased levels of methyl bromide in body fluids. Under these findings of methyl bromide intoxication, erectile dysfunction was diagnosed with a neurological origin.…”

Section: Discussionmentioning

confidence: 81%

A case of erectile dysfunction associated with chronic methyl bromide intoxication

et al. 2004

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Photometric determination of human serum bromide levels—a convenient biomonitoring parameter for methyl bromide exposure

Cited by 27 publications

References 3 publications

Phenotyping of human glutathione S -transferase hGSTT1-1: a comparison of two ex vivo routine procedures

Phenotyping of human glutathione S -transferase hGSTT1-1: a comparison of two ex vivo routine procedures

Determination of bromide in whole blood and urine from humans using gas chromatography–mass spectrometry

A case of erectile dysfunction associated with chronic methyl bromide intoxication

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