Photoluminescent Lateral-Flow Immunoassay Revealed by Graphene Oxide: Highly Sensitive Paper-Based Pathogen Detection

Morales‐Narváez, Eden; Naghdi, Tina; Zor, Erhan; Merkoçi, Arben

doi:10.1021/acs.analchem.5b02383

Cited by 158 publications

(82 citation statements)

References 28 publications

(47 reference statements)

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“…The device LOD was 10 CFU/mL in buffer and 100 CFU/mL in bottled water and milk. 126 Graphene can also be used as a substrate to improve the optical biosensor's performance when conjugated with conventional plasmonic material to obtain high sensitivity by amplifying the detectable signal up to single molecule level detection. For example, the measurement of immunoglobulin G (IgG) antibody level is important to deal with immune deficiencies, autoimmune disorders like autoimmune hepatitis and infections.…”

Section: Graphene Based Optical Biosensing Systemmentioning

confidence: 99%

Graphene based biosensors—Accelerating medical diagnostics to new-dimensions

2017

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Graphene has emerged as a champion material for a variety of applications cutting across multiple disciplines in science and engineering. Graphene and its derivatives have displayed huge potential as a biosensing material due to their unique physicochemical properties, good electrical conductivity, optical properties, biocompatibility, ease of functionalization, and flexibility. Their widespread use in making biosensors has opened up new possibilities for early diagnosis of life-threatening diseases and real-time health monitoring. Following an introduction and discussion on the significance of fabrication protocols and assembly, this review is intended to assess why graphene is suitable to build better biosensors, the working of existing biosensing schemes and their current status toward commercialization for wearable diagnostic and prognostic devices. We believe this review will provide a critical insight for harnessing graphene as a suitable biosensor for the clinical diagnostics, its future prospects and challenges ahead.

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Section: Graphene Based Optical Biosensing Systemmentioning

confidence: 99%

Graphene based biosensors—Accelerating medical diagnostics to new-dimensions

2017

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“…30 These concomitant developments in multiple areas have provided a better understanding of the interactions between biomolecules, biomolecules-labels, biomolecules and/or reagents and paper surfaces, as well as, their interaction mechanisms and their exploitation for improving the performance of the devices. Actually, paper-based assays have been widely applied in multiple areas 4,6,18 such as medicine 1,38 (clinical monitoring and diagnosis of diseases), [39][40][41][42] veterinary, 43 food industry, [44][45][46] agriculture/aquaculture, 28,47,48 environmental safety [49][50][51] and security, 45,52,53 for a large range of analytes: drugs, 28,44,45,52 markets of illness 54 (e. g. tumoral biomarkers, [55][56][57] cardiovascular biomarkers 58 ), proteins in corporal fluids (BSA, 41 thrombin 59 ), hormones, 60,61 bacteria, 28,32,[62][63][64] virus, [65][66][67][68] and chemical contaminants 50,…”

Section: Why Such a Popularity Of Pads?mentioning

confidence: 99%

Paper-based sensors and assays: a success of the engineering design and the convergence of knowledge areas

2016

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This review shows the recent advances and state of the art in paper-based analytical devices (PADs) through the analysis of their integration with microfluidics and LOC micro- and nanotechnologies, electrochemical/optical detection and electronic devices as the convergence of various knowledge areas. The important role of the paper design/architecture in the improvement of the performance of sensor devices is discussed. The discussion is fundamentally based on μPADs as the new generation of paper-based (bio)sensors. Data about the scientific publication ranking of PADs, illustrating their increase as an experimental research topic in the past years, are supplied. In addition, an analysis of the simultaneous evolution of PADs in academic lab research and industrial commercialization highlighting the parallelism of the technological transfer from academia to industry is displayed. A general overview of the market behaviour, the leading industries in the sector and their commercialized devices is given. Finally, personal opinions of the authors about future perspectives and tendencies in the design and fabrication technology of PADs are disclosed.

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“…Nanomaterials combined with paper-based substrates have brought important rewards in the optical sensing field 7–9 . The most widely known assays include dipstick, lateral flow and microfluidic paper-based analytical devices (μPADs) 10 .…”

Section: Introductionmentioning

confidence: 99%

Paper strip-embedded graphene quantum dots: a screening device with a smartphone readout

Álvarez-Diduk

Orozco

Merkoçi

2017

Sci Rep

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Simple, inexpensive and rapid sensing systems are very demanded for a myriad of uses. Intrinsic properties of emerging paper-based analytical devices have demonstrated considerable potential to fulfill such demand. This work reports an easy-to-use, low cost, and disposable paper-based sensing device for rapid chemical screening with a smartphone readout. The device comprises luminescent graphene quantum dots (GQDs) sensing probes embedded into a nitrocellulose matrix where the resonance energy transfer phenomenon seems to be the sensing mechanism. The GQDs probes were synthesized from citric acid by a pyrolysis procedure, further physisorbed and confined into small wax-traced spots on the nitrocellulose substrate. The GQDs were excited by an UV LED, this, is powered by a smartphone used as both; energy source and imaging capture. The LED was contained within a 3D-printed dark chamber that isolates the paper platform from external light fluctuations leading to highly reproducible data. The cellulose-based device was proven as a promising screening tool for phenols and polyphenols in environmental and food samples, respectively. It opens up new opportunities for simple and fast screening of organic compounds and offers numerous possibilities for versatile applications. It can be especially useful in remote settings where sophisticated instrumentation is not always available.

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Photoluminescent Lateral-Flow Immunoassay Revealed by Graphene Oxide: Highly Sensitive Paper-Based Pathogen Detection

Cited by 158 publications

References 28 publications

Graphene based biosensors—Accelerating medical diagnostics to new-dimensions

Graphene based biosensors—Accelerating medical diagnostics to new-dimensions

Paper-based sensors and assays: a success of the engineering design and the convergence of knowledge areas

Paper strip-embedded graphene quantum dots: a screening device with a smartphone readout

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