Photoluminescent Inorganic Nanoprobe‐based Pathogen Detection

Infectious disease mortality has decreased due to effective drugs and healthcare. However, global health remains threatened by infectious diseases. New methods of rapid and accurate bacterial detection have attracted considerable attention. Fluorescence detection of whole bacterial cells offers high sensitivity, quantitative analysis, and simple operation. A highly fluorescent bioconjugated probe improves sensitivity and selectivity. This study presents a novel, bright fluorescent probe comprising a bacteriophage and a fluorescent nanoemulsion (fNE) as biorecognition and signal transduction elements, respectively. We demonstrate that fluorescence microscopy imaging using the S. aureus-specific phage, S13′-fNE (phage-fNE), detects S. aureus in the presence of E. coli or S. pseudintermedius, another closely related Staphylococci, in a highly selective manner. Furthermore, fNEs with high dye loadings exhibit considerably greater brightness compared to the fluorescent dye alone, making them suitable for sensitive fluorescence imaging. Phage-fNEs can quantitatively detect S. aureus at 104–108 colony-forming units per milliliter (CFU mL−1), with a limit of detection of 8 × 104 CFU mL−1. This result is comparable to the lowest value achieved by microscopic bacterial detection, with no preconcentration or enzymatic signal enhancement methods used. Bioconjugated fNEs open new avenues for highly selective and sensitive fluorescent detection of bacteria.

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Recent Advances in Nanobiotechnology for the Treatment of Non-Hodgkin’s Lymphoma

Liu,

Xu,

Zhong

et al. 2024

MRMC

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Lymphoma is the eighth most common type of cancer worldwide. Currently, lymphoma is mainly classified into two main groups: Hodgkin's lymphoma (HL) and non-Hodgkin's lymphoma (NHL), with NHL accounting for 80% to 90% of the cases. NHL is primarily divided into B, T, and natural killer (NK) cell lymphoma. Nanotechnology is developing rapidly and has made significant contributions to the field of medicine. This review summarizes the advancements of nanobiotechnology in recent years and its applications in the treatment of NHL, especially in diffuse large B cell lymphoma (DLBCL), central nerve cell lymphoma, and follicular lymphoma. The technologies discussed include clinical imaging, targeted drug delivery, photodynamic therapy (PDT), and thermodynamic therapy for lymphoma. This review aims to provide a better understanding of the use of nanotechnology in the treatment of non-Hodgkin's lymphoma.

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Photoluminescent Inorganic Nanoprobe‐based Pathogen Detection

Cited by 3 publications

References 76 publications

Applications of Nanomaterials in Medicine: Current Status and Future Scope

Applications of Nanomaterials in Medicine: Current Status and Future Scope

Bacteriophage-Conjugated Fluorescent Nanoemulsion as a Novel Optical Probe for Highly Selective Bacterial Detection

Recent Advances in Nanobiotechnology for the Treatment of Non-Hodgkin’s Lymphoma

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