“…The use of microwaves significantly shortens the synthesis procedure to only 30 minutes making the synthesis more attractive and less time-consuming than room-temperature approaches which take considerably more time. Additionally, as previously shown, 20 His-AuNCs obtained using microwaves exhibit a four times stronger PL emission, at the same excitation, compared to His-AuNCs obtained at room temperature. Afterwards, the His-AuNCs were purified by centrifugation (6000 rpm, 10 min) using a 10 kDa Satorious centrifugal concentrator (Hettich, Germany) to eliminate the surplus of His and unreduced ions.…”