Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells

Roy, Prathik; Periasamy, Arun Prakash; Lin, Chiu Ya; Her, Guor Mour; Chiu, Wei Jane; Li, Chi Lin; Shu, Chia Lun; Huang, Chih‐Ching; Liang, Chi‐Te; Chang, Huan‐Tsung

doi:10.1039/c4nr07005d

Cited by 100 publications

(42 citation statements)

References 39 publications

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“…Recently, Roy et al developed a novel method using NG modified with Annexin V antibody (AbA5) to form (AbA5)-modified NG (AbA5-NG) enabling to label apoptotic cells in live zebrafish (Danio rerio) [ 69 ]. Zebrafish showed bright red photoluminescence in the presence of apoptotic cells.…”

Section: Biosensing Cancer Biomolecules or Cellsmentioning

confidence: 99%

Graphene: The Missing Piece for Cancer Diagnosis?

Cruz

Girão

Gonçalves

et al. 2016

Sensors

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This paper reviews recent advances in graphene-based biosensors development in order to obtain smaller and more portable devices with better performance for earlier cancer detection. In fact, the potential of Graphene for sensitive detection and chemical/biological free-label applications results from its exceptional physicochemical properties such as high electrical and thermal conductivity, aspect-ratio, optical transparency and remarkable mechanical and chemical stability. Herein we start by providing a general overview of the types of graphene and its derivatives, briefly describing the synthesis procedure and main properties. It follows the reference to different routes to engineer the graphene surface for sensing applications with organic biomolecules and nanoparticles for the development of advanced biosensing platforms able to detect/quantify the characteristic cancer biomolecules in biological fluids or overexpressed on cancerous cells surface with elevated sensitivity, selectivity and stability. We then describe the application of graphene in optical imaging methods such as photoluminescence and Raman imaging, electrochemical sensors for enzymatic biosensing, DNA sensing, and immunosensing. The bioquantification of cancer biomarkers and cells is finally discussed, particularly electrochemical methods such as voltammetry and amperometry which are generally adopted transducing techniques for the development of graphene based sensors for biosensing due to their simplicity, high sensitivity and low-cost. To close, we discuss the major challenges that graphene based biosensors must overcome in order to reach the necessary standards for the early detection of cancer biomarkers by providing reliable information about the patient disease stage.

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Section: Biosensing Cancer Biomolecules or Cellsmentioning

confidence: 99%

Graphene: The Missing Piece for Cancer Diagnosis?

Cruz

Girão

Gonçalves

et al. 2016

Sensors

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“…4 In addition, the graphene structure inherent in GQDs endows them with some of the unusual properties of graphene. For these reason, GQDs are proposed to be applicable in various fields, such as biological imaging, [9][10][11] sensing, 12 drug delivery, 13 catalysis, 14,15 and photovoltaics. 16 The strategies for the preparation of GQDs can be divided into two major categories: top-down and bottom-up approaches.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging

Hong

Zhao

Deng

et al. 2018

IJN

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BackgroundThe preparation and biological applications of ultra-small graphene quantum dots (GQDs) with accurate-controlled size are of great significance.MethodsHere in, we report a novel procedure involving pyrolysis of trisodium citrate and subsequent ultrafiltration for fabricating monolayer GQDs with ultra-small lateral size (1.3±0.5 nm).ResultsThe GQDs exhibit blue photoluminescence with peak position independent of excitation wavelength. The quantum yield of GQDs is measured to be 3.6%, and the average fluorescence lifetime is 2.78 ns.ConclusionBecause of high stability and low toxicity, GQDs are demonstrated to be excellent bioimaging agents. The ultra-small GQDs can not only distribute in the cytoplasm but also penetrate into the nuclei. We ensure that this work will add a new dimension to the application of graphene materials for nanomedicine.

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“…[ 14 , 15 ] Recent studies showed that the surface-modified nanoparticles can penetrate into the apoptotic cells, shedding light on the new field of CDots application. [ 16 , 17 ] Also, the ability to visualize model membranes [ 18 ] and cells [ 19 , 20 ] with carbon dots was shown. Meantime, in our knowledge, there were no attempts to apply carbon nanoparticles to visualize the changes of cell functional states, such as apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Visualization and detection of live and apoptotic cells with fluorescent carbon nanoparticles

2015

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Apoptosis is a genetically encoded cell death program that involves different processes occurring on molecular and sub-cellular levels. Here we report on its new features—the increased accumulation of fluorescent carbon nanoparticles (CDots) in cells and their changed distribution within cell interior, which can witness on altered mechanisms of their translocation through the membrane. The comparative studies of living (intact) and apoptotic cells were provided with two cell lines (HeLa, Vero) using two types of fluorescent nanoparticles (“violet” and “blue” CDots). In all studied cases the images of living and apoptotic cells were different; the apoptotic cells incorporated larger number of CDots resulting in their much brighter images. These nanoparticles are distributed in cell cytoplasm, however, when the cells are fixed and treated with detergent, their nucleus is also labeled. Flow cytometry allows distinguishing the sub-populations of living and apoptotic cells in their cultures and suggests a very cheap and easy way to characterize them.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-015-0148-7) contains supplementary material, which is available to authorized users.

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Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells

Cited by 100 publications

References 39 publications

Graphene: The Missing Piece for Cancer Diagnosis?

Graphene: The Missing Piece for Cancer Diagnosis?

Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging

Visualization and detection of live and apoptotic cells with fluorescent carbon nanoparticles

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