Photoluminescence intensity ratio of Eu‐conjugated lactates—A simple optical imaging technique for biomarker analysis for critical diseases

Kakkar, Tarun; Thomas, Nikita; Kumi-Barimah, Eric; Jose, Gin; Saha, Sikha

doi:10.1002/jbio.201700199

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…Nevertheless, we recommend using the 592 nm Eu 3+ emission band because its magnetic dipole nature makes it less sensitive to the environment. 65 The initial rates, obtained by analyzing the enhancement of the luminescence intensity at 592 nm, exhibited an excellent linear relationship with the initial concentration of GSH (in a logarithmic scale) ( Figure S9 ). The linear fitting yielded a slope of 0.52 ± 0.07.…”

Section: Resultsmentioning

confidence: 89%

“…These results open the door to the use of 2b as a self-assembled europium sensitizer to selectively report on the levels of GSH in real time, allowing the reaction to be monitored in situ at different wavelengths, broadening the palette for multiplexing applications. Nevertheless, we recommend using the 592 nm Eu 3+ emission band because its magnetic dipole nature makes it less sensitive to the environment . The initial rates, obtained by analyzing the enhancement of the luminescence intensity at 592 nm, exhibited an excellent linear relationship with the initial concentration of GSH (in a logarithmic scale) (Figure S9).…”

Section: Resultsmentioning

confidence: 99%

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Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells

et al. 2022

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The small molecule 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-carboxylic acid ( 2b ) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu 3+ antenna, after self-assembling with this cation in water. This behavior makes 2b a highly selective GSH biosensor, which has demonstrated high potential for studies in murine and human cells of the immune system (CD4 + T, CD8 + T, and B cells) using flow cytometry. GSH can be monitored by the fluorescence of the product of addition to 2b (445 nm) or by the luminescence of Eu 3+ (592 nm). 2b was able to capture baseline differences in GSH intracellular levels among murine and human CD4 + T, CD8 + T, and B cells. We also successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of CD4 + naïve T cells into regulatory T cells (T REG ).

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells

et al. 2022

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“…The PLIR is defined by the ratio I(604-640 nm)/I(570-604 nm) which is the ratio of integrated intensity of electric dipole transition to magnetic dipole transition [42]. It provides information about the distortion from local sites inversion symmetry of the Eu 3+ in the chelator complex.…”

Section: Fluorescence Of Chelated Eu 3+ To Pmda Modified Affimers Commentioning

confidence: 99%

Affimer-Based Europium Chelates Allow Sensitive Optical Biosensing in a Range of Human Disease Biomarkers

Al-Enezi

Vakurov

Eades

et al. 2021

Sensors

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The protein biomarker measurement has been well-established using ELISA (enzyme-linked immunosorbent assay), which offers good sensitivity and specificity, but remains slow and expensive. Certain clinical conditions, where rapid measurement or immediate confirmation of a biomarker is paramount for treatment, necessitate more rapid analysis. Biosensors offer the prospect of reagent-less, processing-free measurements at the patient’s bedside. Here, we report a platform for biosensing based on chelated Eu3+ against a range of proteins including biomarkers of cardiac injury (human myoglobin), stroke (glial fibrillary acidic protein (GFAP)), inflammation (C-reactive protein (CRP)) and colorectal cancer (carcinoembryonic antigen (CEA)). The Eu3+ ions are chelated by modified synthetic binding proteins (Affimers), which offer an alternative targeting strategy to existing antibodies. The fluorescence characteristics of the Eu3+ complex with modified Affimers against human myoglobin, GFAP, CRP and CEA were measured in human serum using λex = 395 nm, λem = 590 and 615 nm. The Eu3+-Affimer based complex allowed sensitive detection of human myoglobin, GFAP, CRP and CEA proteins as low as 100 fM in (100-fold) diluted human serum samples. The unique dependence on Eu3+ fluorescence in the visible region (590 and 615 nm) was exploited in this study to allow rapid measurement of the analyte concentration, with measurements in 2 to 3 min. These data demonstrate that the Affimer based Eu3+ complexes can function as nanobiosensors with potential analytical and diagnostic applications.

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“…Suitable ligands can serve as efficient antennae to sensitize the 4f-4f transitions and protect Ln III against exogenous binders. 24,[45][46][47] This principle can be extended to incorporate Ln III into macromolecular systems such as nanoparticles, nanofibres or polymers to endow them with favourable properties. 11,[48][49][50][51] In particular, we now describe how incorporating Ln III into chiral complexes can induce CPL as a 'hidden fingerprint'an advanced security feature in the already distinct total luminescence spectra of Ln III (Fig.…”

Section: [H1] Introductionmentioning

confidence: 99%

Circularly polarized lanthanide luminescence for advanced security inks

2020

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Authenticating products and documents with security inks is vital to global commerce, security and health. Lanthanide complexes are widely used in luminescent security inks due to their unique and robust photophysical properties. Lanthanide complexes can also be engineered to undergo circularly polarised luminescence (CPL), which encodes chiral molecular fingerprints in luminescence spectra that cannot be decoded by conventional optical measurements. However, chiral CPL signals have not yet been exploited as an extra security layer in advanced security inks. This Review introduces CPL and related concepts that are necessary to appreciate the challenges and potential of lanthanide-based CPL-active security inks. We describe recent advances in CPL analysis and read-out technologies that have expedited CPL-active security ink applications. Further, we provide a systematic meta-analysis of strongly CPL-active Eu III , Tb III , Sm III , Yb III , Cm III , Dy III and Cr III complexes, discussing the suitability of their photophysical properties and highlighting promising candidates. We conclude by providing key recommendations for the development and advancement of the field.

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Photoluminescence intensity ratio of Eu‐conjugated lactates—A simple optical imaging technique for biomarker analysis for critical diseases

Cited by 5 publications

References 38 publications

Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells

Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells

Affimer-Based Europium Chelates Allow Sensitive Optical Biosensing in a Range of Human Disease Biomarkers

Circularly polarized lanthanide luminescence for advanced security inks

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