Photoinhibition and Reactivation of Photosynthesis in the Cyanobacterium <i>Anacystis nidulans</i>

Samuelsson, Göran; Lönneborg, Anders; Rosenqvist, Eva; Gustafsson, Peter; Öquist, Gunnar

doi:10.1104/pp.79.4.992

Cited by 140 publications

(80 citation statements)

References 24 publications

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“…data), or may be largely absent (Neale et al 1998b). During exposure to polychromatic UV ϩ PAR, a range of potential repair processes (Neale et al 1994) is stimulated by longer wavelengths, thus counteracting the damaging effects of UV radiation (Hirosawa and Miyachi 1983;Samuelsson et al 1985;Neale 1987). Low temperatures have also been hypothesized to enhance the effects of photoinhibition, most likely because of slower rates of repair (Neale et al 1994;Lesser et al 1996).…”

Section: Datementioning

confidence: 99%

Ultraviolet radiation sensitivity of photosynthesis in phytoplankton from an estuarine environment

Banaszak

Neale

2001

Limnology & Oceanography

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We have studied temporal variation in the sensitivity of phytoplankton photosynthesis to inhibition by ultraviolet radiation (UV; 280-400 nm) using biological weighting functions (BWFs) that quantify the biological effect of different wavelengths of UV. Variations in irradiance-dependent BWFs were evaluated for natural phytoplankton assemblages from the Rhode River, a shallow subestuary of the Chesapeake Bay, Maryland, from October 1994 to July 1996. Phytoplankton assemblages were sensitive to UV throughout the year. Rhode River assemblages are inhibited more strongly in the UV-B (280-320 nm), particularly below 300 nm, but there is a significant influence well into the UV-A (320-400 nm). There was no inhibition of phytoplankton photosynthesis by photosynthetically available radiation (400-700 nm), but there was significant seasonal variation in the saturated rate of photosynthesis (P ) and in the light saturation parameter (E s ). There was little variation in seasonal average BWFs through the Ϫ2 ]) and in the spectral shape or relative effect of UV-B versus UV-A, which may be due to changes in species composition, light, temperature, and nutrient availability. Comparison of the most sensitive assemblage (spring) with the least sensitive assemblage (winter) indicates that these BWFs are close to the upper and lower bounds in sensitivity for irradiance-dependent BWFs from all natural and cultured phytoplankton populations. The average, absolute spectral weightings for inhibition of photosynthesis in assemblages from the Rhode River are similar to an average BWF for Antarctic assemblages.

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Section: Datementioning

confidence: 99%

Ultraviolet radiation sensitivity of photosynthesis in phytoplankton from an estuarine environment

Banaszak

Neale

2001

Limnology & Oceanography

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“…We Photoinhibition of photosynthesis may occur when plants are exposed to excessive light (9). We showed in an earlier work (11) that the cyanobacterium Anacystis nidulans responded to photoinhibitory treatments with changes in the function of the photosynthetic apparatus that are typical for higher plants (6,9) and algae (7,10) i.e. inhibition at, or close to, the reaction center of PSII.…”

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confidence: 94%

The Susceptibility of Photosynthesis to Photoinhibition and the Capacity of Recovery in High and Low Light Grown Cyanobacteria, Anacystis nidulans

Samuelsson¹,

Lönneborg²,

Gustafsson³

et al. 1987

Plant Physiol.

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104

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ABSTRACIThe susceptibility of photosynthesis to photoinhibition and the rate of its recovery were studied in the cyanobacterium Anacystis nidulans grown at a low (10 micromoles per square meter per second) and a high (120 micromoles per square meter per second) photosynthetically active radiation. The Photoinhibition of photosynthesis may occur when plants are exposed to excessive light (9). We showed in an earlier work (11) that the cyanobacterium Anacystis nidulans responded to photoinhibitory treatments with changes in the function of the photosynthetic apparatus that are typical for higher plants (6, 9) and algae (7, 10) i.e. inhibition at, or close to, the reaction center of PSII. Although there are evidences that one site of inhibition is at the QB-protein (4, 7) there is still no agreement on the molecular mechanism of photoinhibition, By studying photoinhibition ofphotosynthesis and its recovery in A. nidulans in the presence and absence of transcription and translation inhibitors, we ( 11) and others (3, 4, 7) have given evidence for the existence of a repairing process that restores efficient photosynthesis in dim light. From the results of our own work ( 11) we suggested that the extent ofphotoinhibitory damage observed is the net result of a balance between the photodamage and the operation of a repairing process.It is well documented that the susceptibility to photoinhibition is higher in shade adapted than in sun adapated plants (1, 2, 9). These different susceptibilities have been ascribed to sun plants having higher rates of light saturated photosynthesis than shade plants, thereby giving the sun plants a higher capacity to deexcitate excited Chl in strong lights. However, accepting the hypotheses that the capacity ofa recovery process also is important for net photoinhibition to occur (1 1), it was of interest to investigate if high light acclimated cells have a higher capacity of the recovery process than have low light acclimated plants.For this purpose we have grown A. nidulans at two different light levels and assessed the capacity of the recovery process by adding streptomycin to the two algal cultures during photoinhibition and recovery. We show that high light acclimated A. nidulans have a much higher rate of recovery of photosynthesis after photoinhibition than have low light acclimated A. nidulans. We concluded that the difference in the susceptibility to photoinhibition oflow and high light grown A. nidulans to a significant extent is determined by different capacities of a recovery process operating under excessive light exposure, MATERIALS AND METHODS Culture Conditions. The cyanobacterium Anacystis nidulans 625 (Synechococcus 6301) was grown in batch cultures in an inorganic medium (12) as described earlier (8). The culture was in equilibrivm with air which gave cells adapted to low carbon.The temperature was kept at 38°C and the cultures were exposed to continuous PAR of a low (10 umol m-2s-') and a relatively high (120 Amol m-2s-') photon flux density, respectively (Li-Cor quant...

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“…This light-mediated inhibition resulting from Na+ or Ca2' depletion occurs within PSII, at a site near to the reaction center (7). Illumination at the same PFD utilized for growth is sufficient to elicit inhibition in ion-deficient medium, but the inhibitory mechanism may reflect the same biochemical events as photoinhibition in cells subjected to very high light intensity in complete (Na+-and Ca2+-containing) medium (7,22). If PSII in Na+ and/or Ca2"-deficient medium can be described as photoinhibition sensitized by ion stress.…”

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confidence: 99%

Sequential Events in the Photoinhibition of Synechocystis under Sodium Stress

Zhao¹,

Brand²

1989

Plant Physiol.

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Synechocystis (PCC 6714) is photoinhibited at low photon flux density when illuminated in medium devoid of Na . Photosynthetic activity and variable fluorescence yield were measured in intact cells during the course of inhibition and during recovery from inhibition in order to characterize sequential inhibitory events. Photosystem II antenna chlorophyll becomes functionally disconnected from the reaction center in the first stage of inhibition, although energy is still transferred from phycobilin pigments to the reaction center. This occurs simultaneously with, or subsequent to, the previously observed (J Zhao, JJ Brand [1988] Arch Biochem Biophys 264: 657-664) inactivation of 02-evolution units in Nat-stressed cells. Addition of Na+ to the culture medium rapidly restores the 02-evolving system, but reassociation of photosystem 11 antenna chlorophyll is much slower and requires new protein synthesis. A site nearer to the reaction center of photosystem 11 becomes inactivated in a secondary inhibitory event. Recovery from secondary inhibition occurs slowly and requires protein synthesis. Cells recovering from secondary inhibition produce active photosystem 11 units with a fully functional complement of antenna chlorophyll. Synechocystis is photoinhibited in complete (Na+-containing) medium when illuminated at high photon flux density. These photoinhibited cells exhibit the same recovery behavior as cells at the second stage of inhibition in Na -deficient medium.

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Photoinhibition and Reactivation of Photosynthesis in the Cyanobacterium Anacystis nidulans

Cited by 140 publications

References 24 publications

Ultraviolet radiation sensitivity of photosynthesis in phytoplankton from an estuarine environment

Ultraviolet radiation sensitivity of photosynthesis in phytoplankton from an estuarine environment

The Susceptibility of Photosynthesis to Photoinhibition and the Capacity of Recovery in High and Low Light Grown Cyanobacteria, Anacystis nidulans

Sequential Events in the Photoinhibition of Synechocystis under Sodium Stress

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