Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)₂(NO₂)₂OH]

Abstract

Inhibition of DNA repair enzymes by the ruthenium nitrosyl complex occurs only after light irradiation and is determined by the interactions between the enzyme and active photolysis products.

Solution and solid-state light-induced transformations in heterometallic vanadium-ruthenium nitrosyl complex

“…Commonly, in the absorption spectrum the decrease in the UV-blue range and the corresponding increase of the absorption in red range after light exposure of the complexes in solutions is observed [8,50,82,83]. Since five-coordinated ruthenium complexes are not stable, the free coordination place after NO photo-release is occupied by the solvent molecule [1].…”

“…Earlier, ruthenium nitrosyl complexes [RuNOL 2 (NO 2 ) 2 OH] (L = pyridine, β-picoline) have been studied on the NO release in solution upon irradiation [8,50]. It was shown, the quantum yield of the nitric oxide release reaction was 6-20% depending on the solvent and the excitation wavelength.…”

“…they can be applied in photodynamic therapy for targeted NO delivery. Moreover, ruthenium complexes can interact with DNA repair enzymes, thus, ruthenium nitrosyl complexes are considered as double bioactive species [8]. On the other hand, light irradiation of ruthenium nitrosyl complexes in solid phase induces the formation of metastable state isomers Ru-ON (MS1) or Ru-(η 2 -(NO)) (MS2) from the ground state Ru-NO (GS).…”

Inhibitors of DNA Glycosylases as Prospective Drugs

“…Interestingly, Fpg is strongly inhibited by Cibacron Blue F3GA, a dye bearing structural resemblance to suramin [69] (Table 2). Several papers described the inactivation of Fpg, Nth, OGG1, NEIL1, and NEIL2 by NO-producing agents and suggested damage to redox-sensitive groups in the enzyme molecules [158][159][160][161][162]. However, the mechanisms of this reaction remain unclear, since the known redox-sensitive groups in these glycosylases are different or absent altogether.…”

Photoinduced linkage isomers in a model ruthenium nitrosyl complex: Identification and assignment of vibrational modes

“…The photoexcitation is based on the charge transfer from the metal center to the antibonding empty π*(NO) orbital of the coordinated nitrosyl ligand resulting either in the isomerization of the NO-ligand (formation of linkage isomers) or in the release of nitric oxide [1,2]. Photoinduced linkage isomers in solids can be used in data storage technologies based on holography [3], whereas the NO release from nitrosyl ruthenium complexes in solution renders these complexes attractive for photodynamic therapy applications (PDT), due to generation of highly reactive ruthenium species and free NO [2,[4][5][6].…”