Photoinduced<i>in situ</i>formation of clickable PEG hydrogels and their antibody conjugation

Kahveci, Muhammet U.; Çiftçi, Mustafa; Evran, Serap; Timur, Suna; Yaǧcı, Yusuf

doi:10.1080/15685551.2014.971392

Cited by 11 publications

(10 citation statements)

References 83 publications

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“…Using biotin-streptavidin capture, antibodies were immobilized on PA hydrogels in a robust manner, as opposed to potentially less stable physisorption methods. Although other methods (e.g., click chemistry or the SpyTag-SpyCatcher system) also allow for simple and stable antibody conjugation, they are often more costly and require additional preparation steps by the user (e.g., azido modification of the antibody and incorporation of alkyne groups into the hydrogel for click chemistry, 41 or SpyTag/SpyCatcher protein expression in E. coli and subsequent purification 42 ). In contrast, commercially available biotin- and streptavidin-labeled reagents are more widely available and can be directly used for conjugation in their supplied format.…”

Section: Discussionmentioning

confidence: 99%

A Hydrogel-Integrated Culture Device to Interrogate T Cell Activation with Physicochemical Cues

Chin

Norman

Gentleman

et al. 2020

ACS Appl. Mater. Interfaces

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The recent rise of adoptive T cell therapy (ATCT) as a promising cancer immunotherapy has triggered increased interest in therapeutic T cell bioprocessing. T cell activation is a critical processing step and is known to be modulated by physical parameters, such as substrate stiffness. Nevertheless, relatively little is known about how biophysical factors regulate immune cells, such as T cells. Understanding how T cell activation is modulated by physical and biochemical cues may offer novel methods to control cell behavior for therapeutic cell processing. Inspired by T cell mechanosensitivity, we developed a multiwell, reusable, customizable, two-dimensional (2D) polyacrylamide (PA) hydrogel-integrated culture device to study the physicochemical stimulation of Jurkat T cells. Substrate stiffness and ligand density were tuned by concentrations of the hydrogel cross-linker and antibody in the coating solution, respectively. We cultured Jurkat T cells on 2D hydrogels of different stiffnesses that presented surface-immobilized stimulatory antibodies against CD3 and CD28 and demonstrated that Jurkat T cells stimulated by stiff hydrogels (50.6 ± 15.1 kPa) exhibited significantly higher interleukin-2 (IL-2) secretion, but lower proliferation, than those stimulated by softer hydrogels (7.1 ± 0.4 kPa). In addition, we found that increasing anti-CD3 concentration from 10 to 30 μg/mL led to a significant increase in IL-2 secretion from cells stimulated on 7.1 ± 0.4 and 9.3 ± 2.4 kPa gels. Simultaneous tuning of substrate stiffness and stimulatory ligand density showed that the two parameters synergize (two-way ANOVA interaction effect: p < 0.001) to enhance IL-2 secretion. Our results demonstrate the importance of physical parameters in immune cell stimulation and highlight the potential of designing future immunostimulatory biomaterials that are mechanically tailored to balance stimulatory strength and downstream proliferative capacity of therapeutic T cells.

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Section: Discussionmentioning

confidence: 99%

A Hydrogel-Integrated Culture Device to Interrogate T Cell Activation with Physicochemical Cues

Chin

Norman

Gentleman

et al. 2020

ACS Appl. Mater. Interfaces

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“…However, the inhibition of reaction is completely reversible; the reaction restarts by purging the solution with argon along with photoirradiation. Because of these features, the light-induced CuAAC reactions have been applied extensively to material sciences. ,,,− …”

Section: Light-triggered Azide–alkyne Cycloadditionsmentioning

confidence: 99%

Light-Triggered Click Chemistry

2020

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The merging of click chemistry with discrete photochemical processes has led to the creation of a new class of click reactions, collectively known as photoclick chemistry. These light-triggered click reactions allow the synthesis of diverse organic structures in a rapid and precise manner under mild conditions. Because light offers unparalleled spatiotemporal control over the generation of the reactive intermediates, photoclick chemistry has become an indispensable tool for a wide range of spatially addressable applications including surface functionalization, polymer conjugation and cross-linking, and biomolecular labeling in the native cellular environment. Over the past decade, a growing number of photoclick reactions have been developed, especially those based on the 1,3-dipolar cycloadditions and Diels–Alder reactions owing to their excellent reaction kinetics, selectivity, and biocompatibility. This review summarizes the recent advances in the development of photoclick reactions and their applications in chemical biology and materials science. A particular emphasis is placed on the historical contexts and mechanistic insights into each of the selected reactions. The in-depth discussion presented here should stimulate further development of the field, including the design of new photoactivation modalities, the continuous expansion of λ-orthogonal tandem photoclick chemistry, and the innovative use of these unique tools in bioconjugation and nanomaterial synthesis.

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“…Interestingly, many of these investigations concern the design of antibacterial hydrogels [ 64 ] via thermal free-radical polymerization, and a few have exploited the striking advantages of photochemistry over the thermal process [ 62 , 65 , 66 , 67 ]. Yet, photochemistry is now well recognized as a green method and has attracted great attention because it offers many advantages over thermal processes [ 68 , 69 , 70 ].…”

Section: Introductionmentioning

confidence: 99%

Light and Hydrogels: A New Generation of Antimicrobial Materials

Pierau

Versace

2021

Materials

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Nosocomial diseases are becoming a scourge in hospitals worldwide, and new multidrug-resistant microorganisms are appearing at the forefront, significantly increasing the number of deaths. Innovative solutions must emerge to prevent the imminent health crisis risk, and antibacterial hydrogels are one of them. In addition to this, for the past ten years, photochemistry has become an appealing green process attracting continuous attention from scientists in the scope of sustainable development, as it exhibits many advantages over other methods used in polymer chemistry. Therefore, the combination of antimicrobial hydrogels and light has become a matter of course to design innovative antimicrobial materials. In the present review, we focus on the use of photochemistry to highlight two categories of hydrogels: (a) antibacterial hydrogels synthesized via a free-radical photochemical crosslinking process and (b) chemical hydrogels with light-triggered antibacterial properties. Numerous examples of these new types of hydrogels are described, and some notions of photochemistry are introduced.

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Photoinducedin situformation of clickable PEG hydrogels and their antibody conjugation

Cited by 11 publications

References 83 publications

A Hydrogel-Integrated Culture Device to Interrogate T Cell Activation with Physicochemical Cues

A Hydrogel-Integrated Culture Device to Interrogate T Cell Activation with Physicochemical Cues

Light-Triggered Click Chemistry

Light and Hydrogels: A New Generation of Antimicrobial Materials

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