Photoinduced fragmentation of gas-phase protonated leucine- enkephalin peptide in the VUV range

Ranković, Miloš Lj.; Canon, Francis; Nahon, Laurent; Giuliani, Alexandre; Milosavljević, Aleksandar R.

doi:10.1088/1742-6596/635/1/012034

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…In this particular case for example, GF represents a 12 favored channel at higher photon energies. Note that this domination is even more pronounced with the further increase of the photon energy above 14 eV 31 . The loss of the water molecule from protonated Leu-enk has been detected as a relatively strong fragmentation channel upon both CID 15 and surface-induced dissociation 30 , but this process was not discussed in details.…”

Section: B Photodissociation Ion Yieldsmentioning

confidence: 88%

VUV action spectroscopy of protonated leucine-enkephalin peptide in the 6-14 eV range

Ranković

Canon

Nahon

et al. 2015

The Journal of Chemical Physics

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We have studied the Vacuum Ultraviolet (VUV) photodissociation of gas-phase protonated leucine-enkephalin peptide ion in the 5.7 to 14 eV photon energy range by coupling a linear quadrupole ion trap with a synchrotron radiation source. We report VUV activation tandem mass spectra at 6.7, 8.4, and 12.8 eV photon energies and photodissociation yields for a number of selected fragments. The obtained results provide insight into both near VUV radiation damage and electronic properties of a model peptide. We could distinguish several absorption bands and assign them to particular electronic transitions, according to previous theoretical studies. The photodissociation yields appear to be very different for the various observed fragmentation channels, depending on both the types of fragments and their position along the peptide backbone. The present results are discussed in light of recent gas-phase spectroscopic data on peptides.

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Section: B Photodissociation Ion Yieldsmentioning

confidence: 88%

VUV action spectroscopy of protonated leucine-enkephalin peptide in the 6-14 eV range

Ranković

Canon

Nahon

et al. 2015

The Journal of Chemical Physics

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Synchronized Reverse Scan Collision Induced Dissociation in Digital Ion Trap Mass Spectrometer for Improving Fragment Ion Detection

Wang

Ding

et al. 2022

Anal. Chem.

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Development of fragment ion detection methods is of great importance for mass spectrometer advancement or substance identification. To date, collision induced dissociation (CID) remains the most commonly used ion activation method in MS/MS experiments, and the effectiveness of CID in an ion trap mass spectrometer is limited by low mass cutoff and weak fragmentation yields. Theoretically, controlling the q value is the key to maintain the fragment efficiency and trapping efficiency of MS/MS, thus improving the detection of fragment ion, while currently reported techniques usually require complex circuitry and often produce different CID patterns. In this paper, with the developed synchronized reversed scanning–collision induced dissociation (SRS-CID) technique, we demonstrate its effective improvement in fragment ion detection. The SRS-CID is implemented on a digital ion trap mass spectrometer (DITMS) by reverse scanning the q values during CID process, or specifically, the frequency is increased during the CID process. With the SRS-CID technique, the fragmentation efficiency of precursor ions can be slightly improved. For reserpine analyte, the trapping efficiency for low-mass fragment ions is improved at least 3 times, and for YGGFL, the trapping efficiency for low-mass fragment ions is improved at least 9 times. These experimental results can also be validated by simulations, and the kinetic energy variation plot suggests consecutive fragmentation occurs. In any case, the SRS-CID provides a solution to the low efficiency of fragment ion detection during tandem MS analysis, which will certainly be useful in the future.

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Photoinduced fragmentation of gas-phase protonated leucine- enkephalin peptide in the VUV range

Cited by 2 publications

References 16 publications

VUV action spectroscopy of protonated leucine-enkephalin peptide in the 6-14 eV range

VUV action spectroscopy of protonated leucine-enkephalin peptide in the 6-14 eV range

Synchronized Reverse Scan Collision Induced Dissociation in Digital Ion Trap Mass Spectrometer for Improving Fragment Ion Detection

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