Photografted fluoropolymers as novel chromatographic supports for polymeric monolithic stationary phases

Abstract

In this study, porous polymer monoliths were in situ synthesized in fluoropolymers tubing to prepare microbore HPLC columns. To ensure the formation of robust homogeneous polymer monoliths in these housing supports, the inner surface of fluoropolymer tubing was modified in a two-step photografting process. Raman spectroscopy and scanning electron microscopy (SEM) confirmed the successful modification of the inner poly(ethylene-co-tetrafluoroethylene) (ETFE) wall and the subsequent attachment of a monolith onto the wall. Poly(glycidyl methacrylate-co-divinylbenzene), poly(butyl methacrylate-co-ethyleneglycol dimethacrylate) and poly(styrene-co-divinylbenzene) monoliths were in situ synthesized by thermal polymerization within the confines of surface vinylized ETFE tubes. The resulting monoliths exhibited good permeability and mechanical stability (pressure resistance up to 9 MPa). The chromatographic performance of these different monolithic columns was evaluated via the separation of alkyl benzenes and proteins in a conventional HPLC system.

Construction of polymer monolithic columns in polypropylene ink‐pen tubes for separation of proteins by cation‐exchange chromatography

“…Then, the tubes filled with a solution of 5 wt% BP in methanol, formerly sonicated (10 min), and purged with N 2 (10 min), were closed in both ends and irradiated for 20 min under 254 nm at 120 mJ/cm 2 . In all experiments, the tubes were positioned 2.5 cm apart from the UV‐lamps [9,13]. After this first step, the tubes were washed with methanol and dried with N 2 .…”

“…A polymerization mixture containing 30 wt% GMA, 10 wt% EDMA, 35 wt% 1‐propanol, 20 wt% 1,4 butanediol, and 5 wt% H 2 O was prepared in a 2 mL amber vial in the presence of 1.0 wt% DMPAP (concerning the monomers). The mixture was sonicated (10 min), purged with N 2 (10 min), and used to fill the activated PP tubes, which were closed in both ends, vertically positioned 2.5 cm apart from the UV‐lamps, and irradiated for 20 min under 254 nm at 120 mJ/cm 2 [9,13]. Then the columns were flushed with ACN at 500 µL/min using an HPLC pump until a constant pressure.…”

“…The high porosity of polymer monoliths enables fast separation and purification of large objects such as biomolecules [1] and viruses [2,3] under high flow rates and convective mass transport [4]. Stainless steel, fused silica capillaries [5], fused silica lined stainless steel [6,7], PEEK [8], and fluoropolymer tubes [9] with internal diameters varying from few tens of micrometers to the conventional 4.6 mm served as supports and molds for the monoliths.…”

“…While thermal polymerization takes several hours to achieve acceptable reaction conversion, photopolymerization takes only a few minutes [11,12]. Following this strategy, monolithic columns were recently prepared inside the UV‐transparent ETFE [9,13] and FEP [14] tubing. Polypropylene is a cheap plastic material that houses monolithic materials for solid‐phase extraction inside pipette tips [15] and syringe barrels [16].…”

Photo-graft modification of fluoropolymer surface and its bonding properties with EVA hot melt adhesive

“…未经改性的含氟树脂表面能较 低, 与EVA胶膜粘接强度差, 在与EVA粘接之前通 常要对其表面改性处理以增强其粘接性能 [6] . 对含氟 树脂材料表面改性方法主要有湿化学处理法 [7~9] 、火 焰 法 [10] 、 等 离 子 法 [11,12] 、 电 晕 法 [13,14] 、 辐 射 接 枝 法 [15,16] 、离子注入法 [17] 、激光处理法 [18,19] 和紫外接枝 法 [20,21] As an important part of photovoltaic (PV) modules, backsheet materials play an important role in protecting solar cell from environmental degradation caused by moisture penetration. Fluoropolymers, which have excellent weatherability, UV radiation resistance and chemical stability, are widely used as the outer layer of the backsheet to ensure the long-term reliability and safety of PV modules.…”