Photogenerator of trichloroacetic acid as a promising detritylation agent for oligonucleotide microarray synthesis

Abstract

4 (4 Methoxyphenyl) 2,6 dinitro phenyl](phenyl)methyl] 2,2,2 trichloroacetate, which gen erates trichloroacetic acid when irradiated with light of 405 nm wavelength, has been studied as a detritylation agent in the oligonucleotide microarray synthesis. This agent was shown to be suitable for the deprotection of the 4,4' dimethoxytrityl group during the solid phase oligonucleotide synthesis.