Abstract:Photoreduetion of NAD has been accomplished by a system consi~tin~ of the NAD-depeadent h:cdrogenase from Alcaligenes eutrophus immobilized on CdS particles with formate as artificial electron donor. Enzymatieally active NADH is formed under illumination of this system by visible light. Accumulation of the eoenzyme dimer (NAD), was not detected. NAD photoreduction is supposed to proceed via the direct electron tranffgr from the semiconductor to the enz),me electron transport ehaila. However, NADH formation as … Show more
“…faecalis has versatile functions including degradation of polyaromatic hydrocarbons 21 and plastics 22 , arsenite oxidation 23 , heterotrophic nitrification-aerobic denitrification to remove ammonium in wastewater 24 , siderophore production, and promotion of plant growth in the soil rhizosphere 25 . In the presence of the semiconducting mineral CdS and light, NAD can be photoreduced to NADH via the direct electron transport from CdS to the enzyme transport chain of an Alcaligenes species 26 . The reducing power of NADH has been observed to support the growth of heterotrophic bacteria such as Escherichia coli 27 .…”
Section: Growth Of a Faecalis On Photoelectronsmentioning
“…faecalis has versatile functions including degradation of polyaromatic hydrocarbons 21 and plastics 22 , arsenite oxidation 23 , heterotrophic nitrification-aerobic denitrification to remove ammonium in wastewater 24 , siderophore production, and promotion of plant growth in the soil rhizosphere 25 . In the presence of the semiconducting mineral CdS and light, NAD can be photoreduced to NADH via the direct electron transport from CdS to the enzyme transport chain of an Alcaligenes species 26 . The reducing power of NADH has been observed to support the growth of heterotrophic bacteria such as Escherichia coli 27 .…”
Section: Growth Of a Faecalis On Photoelectronsmentioning
“…In 1992, Shumilin et al (1992) determined that NADH was photogenerated from NAD-dependent hydrogenase extracted from Alcaligenes eutrophus in the presence of semiconducting CdS. Their study provided strong evidence that photoelectrons generated from illuminated CdS particles participated in microbial intracellular biochemical processes at the molecular level.…”
“…, C-and P-doped TiO 2 , CdS, and CdSe, have been used. [7][8][9] To utilize wider range of visible light, graphene oxides covalently attached with benzophenone coupled porphyrins are used as a light collector for reduction of NAD + and conversion of carbon dioxide.…”
Based on the processes occurring in natural Photocatalytic Systems of Pt Nanoparticles and Molecular Co Complexes for NADH Regeneration and Enzyme-coupled CO2 Conversion
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