Photofragmentation of and electron photodetachment from a GFP model chromophore in a quadrupole ion trap

Forbes, Matthew W.; Nagy, Andrea M.; Jockusch, Rebecca A.

doi:10.1016/j.ijms.2011.08.016

Cited by 41 publications

(62 citation statements)

References 55 publications

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“…23 However, given the laser bandwidth and 50 the finite temperature, this seems unlikely. Our observations are consistent with those of the Jockusch 13,14 and Andersen 11, 12 groups. A major channel that is observed in both these studies is fragmentation.…”

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confidence: 93%

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Photoelectron spectroscopy of the model GFP chromophore anion

Horke

Verlet

2012

Phys. Chem. Chem. Phys.

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. (2012) Publisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details.

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confidence: 93%

“…11,12 Recent gas-phase experiments on the isolated HBDI -have shown that the primary 45 deactivation pathways following excitation to the S 1 excited state are electron detachment and fragmentation, but not fluorescence. 13,14 However, yet unknown properties of HBDI -are Fig. 1 HBDI -chromophore and radical formed upon photodetachment.…”

mentioning

confidence: 99%

Photoelectron spectroscopy of the model GFP chromophore anion

Horke

Verlet

2012

Phys. Chem. Chem. Phys.

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“…Studies of HBDI have revealed that the electronic absorption spectrum of the deprotonated chromophore anion in the gas phase is remarkably similar to that in the protein. 10 However, in the gas phase, the deprotonated chromophore anion does not fluoresce 11,12 and its excited state lifetime 13 has been found to be very similar to that in solution. 14 A number of recent photoelectron spectroscopy and theoretical studies of gas-phase HBDI have shown that following excitation to the first electronically excited state of a) h.h.fielding@ucl.ac.uk the deprotonated anion, 1 1 ππ*, direct and indirect photodetachment processes compete with internal conversion and fragmentation.…”

Section: Introductionmentioning

confidence: 99%

Competition between photodetachment and autodetachment of the $\bm {2^1\pi \pi ^}$21ππ state of the green fluorescent protein chromophore anion

Mooney

Parkes

Zhang

et al. 2014

The Journal of Chemical Physics

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Using a combination of photoelectron spectroscopy measurements and quantum chemistry calculations, we have identified competing electron emission processes that contribute to the 350-315 nm photoelectron spectra of the deprotonated green fluorescent protein chromophore anion, phydroxybenzylidene-2,3-dimethylimidazolinone. As well as direct electron detachment from S 0 , we observe resonant excitation of the 2 1 ππ* state of the anion followed by autodetachment. The experimental photoelectron spectra are found to be significantly broader than photoelectron spectrum calculated using the Franck-Condon method and we attribute this to rapid (∼10 fs) vibrational decoherence, or intramolecular vibrational energy redistribution, within the neutral radical.

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“…For biomolecules, and other molecules with suitably facile bonds, a PD action spectrum can provide rich details about the primary molecular structure, electronic excited states and conformation of ions in the gas-phase [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

UV Photodissociation Action Spectroscopy of Haloanilinium Ions in a Linear Quadrupole Ion Trap Mass Spectrometer

Hansen

Kirk

Blanksby

et al. 2013

J. Am. Soc. Mass Spectrom.

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. (2013). UV photodissociation action spectroscopy of haloanilinium ions in a linear quadrupole ion trap mass spectrometer. Journal of the American Society for Mass Spectrometry, 24 (6), 932-940. UV photodissociation action spectroscopy of haloanilinium ions in a linear quadrupole ion trap mass spectrometer Abstract UV-vis photodissociation action spectroscopy is becoming increasingly prevalent because of advances in, and commercial availability of, ion trapping technologies and tunable laser sources. This study outlines in detail an instrumental arrangement, combining a commercial ion-trap mass spectrometer and tunable nanosecond pulsed laser source, for performing fully automated photodissociation action spectroscopy on gas-phase ions. The components of the instrumentation are outlined, including the optical and electronic interfacing, in addition to the control software for automating the experiment and performing online analysis of the spectra. To demonstrate the utility of this ensemble, the photodissociation action spectra of 4-chloroanilinium, 4-bromoanilinium, and 4-iodoanilinium cations are presented and discussed. Multiple photoproducts are detected in each case and the photoproduct yields are followed as a function of laser wavelength. It is shown that the wavelength-dependent partitioning of the halide loss, H loss, and NH3 loss channels can be broadly rationalized in terms of the relative carbon-halide bond dissociation energies and processes of energy redistribution. The photodissociation action spectrum of (phenyl)Ag2 + is compared with a literature spectrum as a further benchmark. AbstractUV-Vis photodissociation action spectroscopy is becoming increasingly prevalent due to advances in, and commercial availability of, ion trapping technologies and tunable laser sources. This study outlines in detail an instrumental arrangement, combining a commercial ion-trap mass spectrometer and tunable nanosecond pulsed laser source, for performing fully automated photodissociation action spectroscopy on gas-phase ions. The components of the instrumentation are outlined, including the optical and electronic interfacing, in addition to the control software for automating the experiment and performing online analysis of the spectra. To demonstrate the utility of this ensemble, the photodissociation action spectra of 4-chloroanilinium, 4-bromoanilinium and 4-iodoanilinium cations are presented and discussed. Multiple photoproducts are detected in each case and the photoproduct yields are followed as a function of laser wavelength. It is shown that the wavelength-dependent partitioning of the halide loss, H loss and NH 3 loss channels can be broadly rationalized in terms of the relative carbon-halide bond dissociation energies and processes of energy redistribution. The photodissociation action spectrum of (phenyl)Ag 2 + is compared to a literature spectrum as a further benchmark.3

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Photofragmentation of and electron photodetachment from a GFP model chromophore in a quadrupole ion trap

Cited by 41 publications

References 55 publications

Photoelectron spectroscopy of the model GFP chromophore anion

Photoelectron spectroscopy of the model GFP chromophore anion

Competition between photodetachment and autodetachment of the $\bm {2^1\pi \pi ^}$21ππ state of the green fluorescent protein chromophore anion

UV Photodissociation Action Spectroscopy of Haloanilinium Ions in a Linear Quadrupole Ion Trap Mass Spectrometer

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Photofragmentation of and electron photodetachment from a GFP model chromophore in a quadrupole ion trap

Cited by 41 publications

References 55 publications

Photoelectron spectroscopy of the model GFP chromophore anion

Photoelectron spectroscopy of the model GFP chromophore anion

Competition between photodetachment and autodetachment of the $\bm {2^1\pi \pi ^*}$21ππ* state of the green fluorescent protein chromophore anion

UV Photodissociation Action Spectroscopy of Haloanilinium Ions in a Linear Quadrupole Ion Trap Mass Spectrometer

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Resources

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Competition between photodetachment and autodetachment of the $\bm {2^1\pi \pi ^}$21ππ state of the green fluorescent protein chromophore anion